Halorientalis salina sp. nov., Halorientalis marina sp. nov., Halorientalis litorea sp. nov.: three extremely halophilic archaea isolated from a salt lake and coarse sea salt

Wang, Beibei; Sun, Yaping; Wu, Zhang-Ping; Zheng, Xi-Wen; Hou, Jing; Cui, Heng‐Lin

doi:10.1007/s00792-022-01275-y

Cited by 15 publications

(5 citation statements)

References 33 publications

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“…Diverse marine environments contain multifarious extremely halophilic archaea requiring high concentrations of salt for growth [1–4]. Salted Laminaria , which is fresh Laminaria preserved with crude marine solar salt, may serve as an appropriate reservoir for halophilic archaea living in hypersaline habitats of marine origin [5].…”

Section: Introductionmentioning

confidence: 99%

Halomicroarcula laminariae sp. nov. and Halomicroarcula marina sp. nov., extremely halophilic archaea isolated from salted brown alga Laminaria and coastal saline-alkali lands

Xue

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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Four extremely halophilic archaeal strains, LYG-108T, LYG-24, DT1T and YSSS71, were isolated from salted Laminaria produced in Lianyungang and saline soil from the coastal beach at Jiangsu, PR China. The four strains were found to be related to the current species of Halomicroarcula (showing 88.1–98.5% and 89.3–93.6% similarities, respectively) as revealed by phylogenetic analysis based on 16S rRNA and rpoB′ genes. These phylogenies were fully supported by the phylogenomic analysis, and the overall genome-related indexes (average nucleotide identity, DNA–DNA hybridization and average amino acid identity) among these four strains and the Halomicroarcula species were 77–84 %, 23–30 % and 71–83 %, respectively, clearly below the threshold values for species demarcation. Additionally, the phylogenomic and comparative genomic analyses revealed that Halomicroarcula salina YGH18T is related to the current species of Haloarcula rather than those of Halomicroarcula , Haloarcula salaria Namwong et al. 2011 is a later heterotypic synonym of Haloarcula argentinensis Ihara et al. 1997, and Haloarcula quadrata Oren et al. 1999 is a later heterotypic synonym of Haloarcula marismortui Oren et al. 1990. The major polar lipids of strains LYG-108T, LYG-24, DT1T and YSSS71 were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulphate, sulphated mannosyl glucosyl diether and additional glycosyl-cardiolipins. All these results showed that strains LYG-108T (=CGMCC 1.13607T=JCM 32950T) and LYG-24 (=CGMCC 1.13605=JCM 32949) represent a new species of the genus Halomicroarcula , for which the name Halomicroarcula laminariae sp. nov. is proposed; strains DT1T (=CGMCC 1.18928T=JCM 35414T) and YSSS71 (=CGMCC 1.18783=JCM 34915) also represent a new species of the genus Halomicroarcula , for which the name Halomicroarcula marina sp. nov. is proposed.

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Section: Introductionmentioning

confidence: 99%

Halomicroarcula laminariae sp. nov. and Halomicroarcula marina sp. nov., extremely halophilic archaea isolated from salted brown alga Laminaria and coastal saline-alkali lands

Xue

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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“…The pH range for growth was tested according to Wang et al . [34]. Utilization of sole carbon, nitrogen and energy sources was tested as described previously [10].…”

Section: Methodsmentioning

confidence: 99%

“…The temperature range for growth was observed by cultivating the halophilic archaeal strains in NHM at 15-60 °C [33]. The pH range for growth was tested according to Wang et al [34]. Utilization of sole carbon, nitrogen and energy sources was tested as described previously [10].…”

Section: Morphological and Physiological Characterizationmentioning

confidence: 99%

Halomicrococcus gelatinilyticus sp. nov. and Halosimplex aquaticum sp. nov., halophilic archaea isolated from saline soil and an inland solar saltern

Hu,

Ma,

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

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Two extremely halophilic archaeal strains, GSLN9T and XZYJT29T, were isolated from the saline soil in different regions of western China. Both strains GSLN9T and XZYJT29T have two 16S rRNA genes with similarities of 95.1 and 94.8 %, respectively. Strain GSLN9T was mostly related to the genus Halomicrococcus based on 16S rRNA (showing 91.0–96.0 % identities) and rpoB′ genes (showing 92.0 % identity). Strain XZYJT29T showed 92.1–97.6 % (16S rRNA gene) and 91.4–93.1 % (rpoB′ gene) sequence similarities to its relatives in the genus Halosimplex , respectively. The polar lipid profile of strain GSLN9T included phosphatidic acid (PA), phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), phosphatidylglycerol sulphate (PGS), sulphated mannosyl glucosyl diether (S-DGD-1) and sulphated galactosyl mannosyl glucosyl diether (S-TGD-1), mostly similar to that of Halomicrococcus hydrotolerans H22T. PA, PG, PGP-Me, S-DGD-1 (S-DGD-PA), S2-DGD, S-TGD-1 and an unidentified glycolipid were detected in strain XZYJT29T; this polar lipid composition is similar to those of members of the genus Halosimplex . The average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity values between these two strains and their relatives of the genera Halomicrococcus and Halosimplex were no more than 82, 27 and 80 %, respectively, much lower than the thresholds for species demarcation. Other phenotypic characterization results indicated that strains GSLN9T and XZYJT29T can be differentiated from the current species of the genera Halomicrococcus and Halosimplex , respectively. These results revealed that strains GSLN9T (=CGMCC 1.15215T=JCM 30842T) and XZYJT29T (=CGMCC 1.15828T=JCM 31853T) represent novel species of Halomicrococcus and Halosimplex , for which the names Halomicrococcus gelatinilyticus sp. nov. and Halosimplex aquaticum sp. nov. are proposed.

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“…The genomic DNA of strains ZS-10 T and GSL13 T was extracted and purified using a genomic DNA extraction kit [10]. The haloarchaeal 16S rRNA genes and RNA polymerase subunit B′ ( rpoB′ ) genes were amplified and sequenced as described previously [11]. The related taxa of strains ZS-10 T and GSL13 T were identified by using the EzBioCloud server and the sequence similarities among them were calculated using the pairwise aligner [12].…”

Section: Methodsmentioning

confidence: 99%

Salinigranum marinum sp. nov. and Halohasta salina sp. nov., halophilic archaea isolated from sediment of a marine saltern and inland saline soil

Cheng,

Li,

Tan

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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Two halophilic archaeal strains, ZS-10T and GSL13T, were isolated from the Zhoushan marine saltern in Zhejiang, and an inland saline soil from the Tarim Basin, Xinjiang, PR China, respectively. The cells of strain ZS-10T were pleomorphic while those of strain GSL13T were rod-shaped. Both of them stained Gram-negative and formed red-pigmented colonies on agar plates and their cells lysed in distilled water. The optimum growth of strain ZS-10T was observed at 40 °C, 3.4 M NaCl, 0.03 M MgCl2 and pH 7.5, while that of strain GSL13T was at 37 °C, 3.1 M NaCl, 0.5 M MgCl2 and pH 7.5. Phylogenetic and phylogenomic analyses indicated that these two strains were related to Salinigranum and Halohasta , respectively. Strains ZS-10T and GSL13T could be differentiated from the current members of Salinigranum and Halohasta based on the comparison of diverse phenotypic characteristics. The average amino acid identity, average nucleotide identity and digital DNA–DNA hybridization values among strain ZS-10T and current species of Salinigranum were 75.8–78.6 %, 80.6–81.9 % and 24.3–26.1 %, respectively. These values between strain GSL13T and current species of Halohasta were 78.4–80.8 %, 79.8–82.8% and 22.7–25.7 %, respectively, clearly below the threshold values for species demarcation. The polar lipids of strain ZS-10T were phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me) and sulphated mannosyl glucosyl diether (S-DGD-1), while those of strain GSL13T were phosphatidic acid, PG, PGP-Me, phosphatidylglycerol sulphate and S-DGD-1. The polar lipid profile of strain GSL13T was identical to those of Halohasta , whereas strain ZS-10T did not contain the minor glycolipids detected in the current Salinigranum species. The phenotypic, phylogenetic and genome-based results suggested that strains ZS-10T (=CGMCC 1.12868T=JCM 30241T) and GSL13T (=CGMCC 1.15214T=JCM 30841T) represent two novel species, for which the names Salinigranum marinum sp. nov. and Halohasta salina sp. nov. are proposed.

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Halorientalis salina sp. nov., Halorientalis marina sp. nov., Halorientalis litorea sp. nov.: three extremely halophilic archaea isolated from a salt lake and coarse sea salt

Cited by 15 publications

References 33 publications

Halomicroarcula laminariae sp. nov. and Halomicroarcula marina sp. nov., extremely halophilic archaea isolated from salted brown alga Laminaria and coastal saline-alkali lands

Halomicroarcula laminariae sp. nov. and Halomicroarcula marina sp. nov., extremely halophilic archaea isolated from salted brown alga Laminaria and coastal saline-alkali lands

Halomicrococcus gelatinilyticus sp. nov. and Halosimplex aquaticum sp. nov., halophilic archaea isolated from saline soil and an inland solar saltern

Salinigranum marinum sp. nov. and Halohasta salina sp. nov., halophilic archaea isolated from sediment of a marine saltern and inland saline soil

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