Halotolerant and metalotolerant bacteria strains with heavy metals biorestoration possibilities isolated from Uburu Salt Lake, Southeastern, Nigeria

Abstract

Environmental contaminations by heavy metals are currently an increasing public health concern globally. One key challenge of these toxic metals is the extremely difficulties involved in their detoxification from the environment and effluents because of their non-degradability. An efficient biologic agent with potentials of remediating these toxic metals may ease these ever-increasing problems. We reported toxic metals tolerance and bioremediation potentials of novel bacteria sp. Strains USL2S, USL4W and USL5W isolated from Uburu salt lake, Ebonyi State, Nigeria. The phenotypic characteristics and the 16S rRNA gene analyses revealed that USL2S strain belongs to the genus Klebsiella, whereas USL4W and USL5W strains belong to the genus Pseudomonas. The bacteria isolates grew well in media containing 5-15 % of sodium chloride. The bacteria isolate showed capacity to tolerate 50.0 mM Hg þ2 and Pb þ2 , 17.0, 12.50 and 4.0mM Ni þ2 , Cd þ2 , and Zn þ2 respectively in solid media. Pseudomonas putida A4W Strain also tolerated 16.0 mM Cu þ2 , while Klebsiella sp. Strain USL2S, Pseudomonas putida USL5W Strain tolerated 4.0 mM each.

Sweat and sebum preferences of the human skin microbiota

“…While the highest NaCl concentration tested in this study only reached 1.05% (4X artificial sweat) and is not considered a high salt concentration, many skin microorganisms are highly halotolerant and capable of withstanding drastic changes in osmotic pressure, including Staphylococcus and Corynebacterium species (47)(48)(49)(50)(51)(52)(53). In addition, nearly all other genera in this study have reports of species being identified as halotolerant (54)(55)(56)(57)(58)(59).…”

Finding the best combination of autochthonous microorganisms with the most effective biosorption ability for heavy metals removal from wastewater

“… Holan and Volesky (1994) stated that sorption of the metals increases with increasing valence and the atomic number of metals, which was partially agreed upon by the current study. Orji et al (2021) investigated the biosorption efficiency of Klebsiella sp. USL25 for removing Hg 2+ , Pb 2+ , Cd 2+ , and Ni 2+ and obtained the following results: 85, 97.13, 73.33, and 86.06%, respectively.…”

Biomineralization by Extremely Halophilic and Metal-Tolerant Community Members from a Sulfate-Dominated Metal-Rich Environment

“…However, former mining sites are associated with non-alkaline or even acidic pH, which promotes metal leaching [12]. At circumneutral pH such as the niche investigated, co-occurrence of (sulfate) salt ions and metal ions imposes extra stress on the microorganisms, which results in co-tolerance [10][11][12].…”

“…However, former mining sites are associated with circum-neutral or even acidic pH, the latter promoting metal leaching. The co-occurrence of metal ions poses another stress on the microbial community, which results in co-tolerance [10,11].…”