Halovulum marinum sp. nov., isolated from deep-sea water of the Indian Ocean, and emended description of the genus Halovulum

Ruan, Chujin; Wang, Jian; Zheng, Xiaowei; Song, Lei; Zhu, Yaxin; Huang, Ying; Lu, Zujun; Du, Wei; Huang, Li; Dai, Xin

doi:10.1099/ijsem.0.004307

Cited by 7 publications

(1 citation statement)

References 26 publications

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“…Sediments were stored at −80 • C and kept on dry ice during transportation. Then, 5 g sediment samples were inoculated into marine 2216E medium [16]. After incubation at 18 • C for 1-2 weeks, the enrichment cultures were plated onto marine agar 2216E.…”

Section: Strain Isolationmentioning

confidence: 99%

Isolation and Characterization of the First Temperate Virus Infecting Psychrobacillus from Marine Sediments

Liu

Zheng

Dai

et al. 2022

Viruses

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Viruses are far more abundant than cellular microorganisms in the marine ecosystem. However, very few viruses have so far been isolated from marine sediments, especially hydrothermal vent sediments, hindering the understanding of the biology and ecological functions of these tiny organisms. Here, we report the isolation and characterization of a temperate bacteriophage, named PVJ1, which infects Psychrobacillus from a hydrothermal vent field in Okinawa Trough. PVJ1 belongs to the Myoviridae family of the order Caudovirales. The tailed phage possesses a 53,187 bp linear dsDNA genome, with 84 ORFs encoding structural proteins, genome replication, host lysis, etc. in a modular pattern. The phage genome is integrated into the host chromosome near the 3′-end of deoD, a gene encoding purine nucleoside phosphorylase (PNP). The phage integration does not appear to disrupt the function of PNP. The phage DNA is packaged by the headful mechanism. Release of PVJ1 from the host cell was drastically enhanced by treatment with mitomycin C. Phages encoding an MCP sharing significant similarity (≥70% identical amino acids) with that of PVJ1 are widespread in diverse environments, including marine and freshwater sediments, soils, artificial ecosystems, and animal intestines, and primarily infect Firmicutes. These results are valuable to the understanding of the lifestyle and host interactions of bacterial viruses at the bottom of the ocean.

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Section: Strain Isolationmentioning

confidence: 99%