Hamster-to-rat cardiac xenografts a useful model for transplantation studies

Kakita, Akira; Blanchard, J; Fortner, Joseph G.

doi:10.1016/0022-4804(75)90113-4

Cited by 17 publications

(4 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At the same time donor-specific antibodies could be demonstrated. With some minor differences, similar findings have been reported in a few other sequential studies (3,7,17). In two of these studies (7, 17) conventional light microscopy was used, while Cramer et al…”

Section: Discussionsupporting

confidence: 90%

An ultrastructural analysis of concordant and discordant cardiac xenografts in unmodified recipients

Nielsen

Kemp

1994

APMIS

View full text Add to dashboard Cite

Ultrastructural changes in endothelial cells in a hamster‐to‐rat and guinea pig‐to‐rat heart transplantation model are described. In the hamster‐to‐rat model, changes were minimal with an increase in cytoplasmic vesicles 4–6 h after transplantation. 16–24 h after transplantation larger vesicles appeared and the basement and cell membranes were less well defined. 42–48 h after transplantation the changes had progressed with destruction of cell membranes and the appearance of extravasated erythrocytes. At the time of complete rejection, changes had further progressed with widespread endothelial cell destruction and infiltration of neutrophilic granulocytes and macrophages, and large amounts of fibrin were present. In the guinea pig‐to‐rat model, changes were characterized by the appearance of platelets in close contact with the endothelium of the capillaries 1–3 min after transplantation. 4–6 min after transplantation the basement membranes as well as the cell membranes were affected with indistinct borders and interruption. Occasionally fusion of platelets and endothelial cell membranes was demonstrated. In the grafts examined 7–9 min after transplantation, changes had further progressed. Massive aggregation of platelets now appeared in relation to remnants of endothelical cells. Signs of microvascular damage appeared in both models, but with different morphology. In hamster grafts, endothelial cell activation is indicated by gradual changes in the cell membranes resulting in vascular damage and infiltration of the grafts by macrophages and neutrophilic granulocytes. In the guinea pig grafts, activation of endothelial cells results in platelet aggregation, formation of microthrombi, and subsequent tissue damage. Even though antibody and complement are involved in both types of rejection the basic mechanisms are different.

show abstract

Section: Discussionsupporting

confidence: 90%

An ultrastructural analysis of concordant and discordant cardiac xenografts in unmodified recipients

Nielsen

Kemp

1994

APMIS

View full text Add to dashboard Cite

show abstract

“…The value of this species combination for xenotransplant experimentation was first reported for hearts by Barker & Billingham (1971) and Kakita et al (1975b) and extended subsequently to orthotopic liver , Valdivia et al 1987, Yamaguchi et al 1990) and kidney models (Miyazawa et al submitted). The events and mechanisms of hamster heart, kidney, and liver rejection in rat recipients have been extensively described (Valdivia et al 1987, Yamaguchi et al 1990, Miyazawa et al submitted, Knechtle et al 1987a, Monden et al 1989, Van Den Bogaerde et al 1991, Cramer et al 1992) with emphasis on the preformed low level hamsterspecific xenoantibodies.…”

Section: Can Xenograft Cellular Rejection Be Controlled?mentioning

confidence: 99%

The Biological Basis of and Strategies for Clinical Xenotransplantation

Starzl

Valdivia

Murase

et al. 1994

Immunological Reviews

View full text Add to dashboard Cite

“…To induce long‐term survival of vascularized xenografts, several immunosuppressive regimens have previously been presented in concordant xenotransplantation models, e.g. the mouse‐to‐rat [1] and the hamster‐to‐rat [2] models. However, characteristics of the graft‐infiltrating lymphocytes and the mechanisms behind the actions of these immunosuppressive protocols are not completely described.…”

Section: Introductionmentioning

confidence: 99%

Intragraft cytokine mRNA expression in rejecting and non‐rejecting vascularized xenografts

Lorant¹,

Krook

Wilton

et al. 2003

Xenotransplantation

View full text Add to dashboard Cite

We hereby conclude that both AVR on day 2 and cell-mediated rejection on day 8 (under DSG treatment) in a concordant cardiac mouse-to-rat xenotransplantation model are associated with an increase of proinflammatory cytokines, T helper 1 (Th1)-associated cytokines as well as IL-10, while immunosuppression with CyA + DSG diminishes the levels of all examined cytokines. Grafts undergoing AVR or cellular rejection are subjected to deposits of both IgM and IgG, although circulating donor specific antibodies are undetectable in serum.

show abstract

Hamster-to-rat cardiac xenografts a useful model for transplantation studies

Cited by 17 publications

References 6 publications

An ultrastructural analysis of concordant and discordant cardiac xenografts in unmodified recipients

An ultrastructural analysis of concordant and discordant cardiac xenografts in unmodified recipients

The Biological Basis of and Strategies for Clinical Xenotransplantation

Intragraft cytokine mRNA expression in rejecting and non‐rejecting vascularized xenografts

Contact Info

Product

Resources

About