Hand-Held Raman Spectrometer-Based Dual Detection of Creatinine and Cortisol in Human Sweat Using Silver Nanoflakes

Kim, Hyun Soo; Kim, Hyun Jung; Lee, Jun Ho; Lee, Taeha; Yun, Jongsu; Lee, Gyudo; Hong, Yoochan

doi:10.1021/acs.analchem.1c02496

Cited by 34 publications

(18 citation statements)

References 27 publications

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“…Sweat is another easily obtained (noninvasive and pain-free) human biofluid that has attracted some interest in SERS studies for various chemical composition analyses. − However, there is limited research on sweat sampling for cancer detection.…”

Section: Sweatmentioning

confidence: 99%

Label-Free Sensing with Metal Nanostructure-Based Surface-Enhanced Raman Spectroscopy for Cancer Diagnosis

Constantinou

Hadjigeorgiou

Abalde‐Cela

et al. 2022

ACS Appl. Nano Mater.

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Surface-Enhanced Raman Spectroscopy (SERS) is a powerful analytical technique for the detection of small analytes with great potential for medical diagnostic applications. Its high sensitivity and excellent molecular specificity, which stems from the unique fingerprint of molecular species, have been applied toward the detection of different types of cancer. The noninvasive and rapid detection offered by SERS highlights its applicability for point-of-care (PoC) deployment for cancer diagnosis, screening, and staging, as well as for predicting tumor recurrence and treatment monitoring. This review provides an overview of the progress in label-free (direct) SERS-based chemical detection for cancer diagnosis with the main focus on the advances in the design and preparation of SERS substrates on the basis of metal nanoparticle structures formed via bottom-up strategies. It begins by introducing a synopsis of the working principles of SERS, including key chemometric approaches for spectroscopic data analysis. Then it introduces the advances of label-free sensing with SERS in cancer diagnosis using biofluids (blood, urine, saliva, sweat) and breath as the detection media. In the end, an outlook of the advances and challenges in cancer diagnosis via SERS is provided.

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Section: Sweatmentioning

confidence: 99%

Label-Free Sensing with Metal Nanostructure-Based Surface-Enhanced Raman Spectroscopy for Cancer Diagnosis

Constantinou

Hadjigeorgiou

Abalde‐Cela

et al. 2022

ACS Appl. Nano Mater.

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“…Kim and co-workers presented a new sensor to simultaneously detect creatinine (an important bioindicator of kidney function) and cortisol in sweat using a portable Raman spectrometer [ 52 ] ( Figure 4 ). They fabricated silver nano snowflakes (SNSFs) on a SERS (surface-enhanced Raman scattering) substrate and hydrophobic filter paper using the polyol method [ 53 ].…”

Section: Optical Nanosensorsmentioning

confidence: 99%

“… ( a ) Detection of stress biomarkers in sweat, ( b ) sweat sampling for the classification of normal and risk groups (reproduced with permission from [ 52 ]. Copyright 2021 American Chemical Society).…”

Section: Figurementioning

confidence: 99%

Nanomaterials for Cortisol Sensing

Sfrazzetto

Santonocito

2022

Nanomaterials

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Space represents one of the most dangerous environments for humans, which can be affected by high stress levels. This can lead to severe physiological problems, such as headaches, gastrointestinal disorders, anxiety, hypertension, depression, and coronary heart diseases. During a stress condition, the human body produces specific hormones, such as dopamine, adrenaline, noradrenaline, and cortisol. In particular, the control of cortisol levels can be related to the stress level of an astronaut, particularly during a long-term space mission. The common analytical methods (HPLC, GC-MS) cannot be used in an extreme environment, such as a space station, due to the steric hindrance of the instruments and the absence of gravity. For these reasons, the development of smart sensing devices with a facile and fast analytical protocol can be extremely useful for space applications. This review summarizes the recent (from 2011) miniaturized sensoristic devices based on nanomaterials (gold and carbon nanoparticles, nanotubes, nanowires, nano-electrodes), which allow rapid and real-time analyses of cortisol levels in biological samples (such as saliva, urine, sweat, and plasma), to monitor the health conditions of humans under extreme stress conditions.

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“…With the availability of different SERS nanotags to generate well-defined and unique signal fingerprints, SERS detection is greatly advantageous for multiplex biosensing at a single excitation wavelength. − Indeed, SERS detection is an ideal alternative for addressing the above-mentioned limitations of multiplex cell–surface protein profiling. Moreover, the recent emergence of portable Raman instruments for material or chemical identification applications has, − for the first time, provided the possibility of developing point-of-need multiplex immunoassays through integration with customized SERS nanotags.…”

Section: Introductionmentioning

confidence: 99%

Tetraplex Immunophenotyping of Cell Surface Proteomes via Synthesized Plasmonic Nanotags and Portable Raman Spectroscopy

2022

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Multiplex immunophenotyping of cell surface proteomes is useful for cell characterization as well as providing valuable information on a patient’s physiological or pathological state. Current methods for multiplex immunophenotyping of cell surface proteomes still have associated technical pitfalls in terms of limited multiplexing capability, challenging result interpretation, and large equipment footprint limited to use in a laboratory setting. Herein, we presented a portable surface-enhanced Raman spectroscopy (SERS) assay for multiplex cell surface immunophenotyping. We synthesized and functionalized customizable SERS nanotags for cell labeling and subsequent signal measurement using a portable Raman spectrometer. We extensively evaluated and validated the analytical assay performance of the portable SERS immunophenotyping assay in two different cellular models (red blood cells and breast cancer cells). In terms of analytical specificity, the cell surface immunophenotyping of both red blood cells and breast cancer cells correlated well with flow cytometry. The portable SERS immunophenotyping assay also has comparable analytical repeatability to flow cytometry, with coefficient of variation values of 21.89–23.33% and 6.88–17.32% for detecting red blood cells and breast cancer cells, respectively. The analytical detection limits were 77 cells/mL for red blood cells and 1–17 cells/mL for breast cancer cells. As an alternative to flow cytometry, the portable SERS immunophenotyping assay demonstrated excellent analytical assay performance and possessed advantages such as quick sample-to-result turnaround time, multiplexing capability, and small equipment footprint.

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Hand-Held Raman Spectrometer-Based Dual Detection of Creatinine and Cortisol in Human Sweat Using Silver Nanoflakes

Cited by 34 publications

References 27 publications

Label-Free Sensing with Metal Nanostructure-Based Surface-Enhanced Raman Spectroscopy for Cancer Diagnosis

Label-Free Sensing with Metal Nanostructure-Based Surface-Enhanced Raman Spectroscopy for Cancer Diagnosis

Nanomaterials for Cortisol Sensing

Tetraplex Immunophenotyping of Cell Surface Proteomes via Synthesized Plasmonic Nanotags and Portable Raman Spectroscopy

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