Hand-portable liquid chromatographic instrumentation

Sharma, Sonika; Tolley, Luke T.; Tolley, H. Dennis; Plistil, Alex; Stearns, Stanley D.; Lee, Milton L.

doi:10.1016/j.chroma.2015.07.119

Cited by 51 publications

(34 citation statements)

References 98 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this Highlights article, a novel approach to capillary LC instrument design is introduced to alleviate some of these concerns. To realize this new design, advances in small, high‐pressure LC pumps and on‐column UV‐absorbance detection (LC‐UV) have been integrated into a compact instrument footprint that is field‐portable . Column robustness issues and extra‐column band broadening related to poor connections in capillary systems are reduced through the use of a novel cartridge design that applies appropriate torque for fluidic connections at pressures up to 10 000 psi.…”

Section: Introductionmentioning

confidence: 99%

Portable capillary liquid chromatography for pharmaceutical and illicit drug analysis

Foster

Xie

Pham

et al. 2020

J of Separation Science

Self Cite

View full text Add to dashboard Cite

A newly developed portable capillary liquid chromatograph was investigated for the separation of various pharmaceutical and illicit drug compounds. The system consists of two high‐pressure syringe pumps capable of delivering capillary‐scale flow rates at pressures up to 10 000 psi. Capillary liquid chromatography columns packed with sub‐2 μm particles are housed in cartridges that can be inserted into the system and easily connected through high‐pressure fluidic contact points by simply applying a specific, predetermined torque rather than using standard fittings and less precise sealing protocols. Several over‐the‐counter analgesic drug separations are demonstrated, along with a simple online measurement of tablet dissolution. Twenty illicit drug compounds were also separated across six targeted drug panels. The results described in this study demonstrate the capability of this compact liquid chromatography instrument to address several important drug‐related applications while simplifying system operation, and greatly reducing solvent usage and waste generation essential for onsite analysis.

show abstract

Section: Introductionmentioning

confidence: 99%

Portable capillary liquid chromatography for pharmaceutical and illicit drug analysis

Foster

Xie

Pham

et al. 2020

J of Separation Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…In recent years, breakthroughs in electrochemistry, lab-on-chip, as well as valve and pump design have allowed miniaturized gradient systems to develop at a faster rate. Sharma et al have published "handportable" LC systems (gradient and isocratic) that utilize a cutting-edge pump and valve design [5][6][7]. Their system is battery-operated, but has a limited gradient volume of 60 μL and its robustness and versatility are not yet fully tested.…”

Section: Introductionmentioning

confidence: 99%

High‐performance liquid chromatographic cartridge with gradient elution capability coupled with UV absorbance detector and mass spectrometer for peptide and protein analysis

Lynch

Chen

et al. 2017

J of Separation Science

View full text Add to dashboard Cite

We discuss the construction and performance of a high-performance liquid chromatography cartridge that we developed that resulted from a culmination of previous research. We have recently developed an innovative approach to creating gradient elutions using dual electroosmotic pumps and a series of three valves. This method has been proved to be the most reproducible and robust in producing gradients compared to our previously tested methods. Using this approach, we have assembled a high-performance liquid chromatography cartridge powered and controlled via a computer. We have successfully coupled the cartridge with an ultraviolet absorbance detector and a mass spectrometer for separating complex protein/peptide samples. The cartridge is readily coupled with other detectors such as electrochemical detector and laser-induced fluorescence detector.

show abstract

“…Several reviews give an overview of the advances made in both column sizes and LC modules [87,93,109,110]. The major advantage of miniaturized LC set-ups is the enhanced sensitivity due to higher efficiencies, lower flow rates and minimized void volumes, which will have a positive influence on the ionization efficiency and the peak shapes [111].…”

Section: (Ii) Trends In Liquid Chromatographymentioning

confidence: 99%

“…When using direct SPME or paper spray ionization, chromatographic separations can be omitted to speed up the analysis (electronic supplementary material, figure S3) [51,122,169]. In combination with miniaturized instrumentation, these ambient ionization techniques have a great potential in bioanalysis and in-field applications [22,109].…”

Section: Applicationsmentioning

confidence: 99%

Quantitative mass spectrometry methods for pharmaceutical analysis

Loos¹,

Schepdael²,

Cabooter³

2016

Phil. Trans. R. Soc. A.

View full text Add to dashboard Cite

Quantitative pharmaceutical analysis is nowadays frequently executed using mass spectrometry. Electrospray ionization coupled to a (hybrid) triple quadrupole mass spectrometer is generally used in combination with solid-phase extraction and liquid chromatography. Furthermore, isotopically labelled standards are often used to correct for ion suppression. The challenges in producing sensitive but reliable quantitative data depend on the instrumentation, sample preparation and hyphenated techniques. In this contribution, different approaches to enhance the ionization efficiencies using modified source geometries and improved ion guidance are provided. Furthermore, possibilities to minimize, assess and correct for matrix interferences caused by co-eluting substances are described. With the focus on pharmaceuticals in the environment and bioanalysis, different separation techniques, trends in liquid chromatography and sample preparation methods to minimize matrix effects and increase sensitivity are discussed. Although highly sensitive methods are generally aimed for to provide automated multi-residue analysis, (less sensitive) miniaturized set-ups have a great potential due to their ability for in-field usage. This article is part of the themed issue ‘Quantitative mass spectrometry’.

show abstract

Hand-portable liquid chromatographic instrumentation

Cited by 51 publications

References 98 publications

Portable capillary liquid chromatography for pharmaceutical and illicit drug analysis

Portable capillary liquid chromatography for pharmaceutical and illicit drug analysis

High‐performance liquid chromatographic cartridge with gradient elution capability coupled with UV absorbance detector and mass spectrometer for peptide and protein analysis

Quantitative mass spectrometry methods for pharmaceutical analysis

Contact Info

Product

Resources

About