Hand-Portable Miniaturized Liquid Chromatography for the Determination of Chlorogenic Acids in Dietary Supplements

Soto, C. Soriano; Ponce-Rodríguez, H.D.; Verdú‐Andrés, J.; Campíns‐Falcó, P.; Herráez‐Hernández, R.

doi:10.3390/antiox11122408

Cited by 4 publications

(3 citation statements)

References 27 publications

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“…In the same way, the present work approached both the AgNP synthesis and its application to CGA determination. On the other hand, the main isomers of CGA have been determined using miniaturized chromatographic techniques and conventional HPLC for their application in quality control due to portability [ 30 ] or robustness [ 31 ]. In the present work, the objective was focused in the major isomer (5-caffeoylquinic or chlorogenic acid) accomplishing a more rapid method with a wide linear range and a possible application to the TAC determination.…”

Section: Resultsmentioning

confidence: 99%

“…Very good LODs using Nano-LC. Higher analysis time in Cap and Nano-LC than portable LC [ 30 ] Green coffee beans ◼ Supercritical/methanolic extraction ◼ Dilution ◼ Sonication (10 min) ◼ Chromatogram (19min) HPLC 27–3666 3.2 (LOQ) 0.6–2 Separation of the CGAs profile (7 compounds). Validation in three independent laboratories.…”

Section: Resultsmentioning

confidence: 99%

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Determination of antioxidant activity by in situ synthesis of AgNPs using in-tube SPME coupled on-line to capillary liquid chromatography

Prieto-Blanco,

Pardo-Puñal,

Moliner-Martínez

et al. 2023

Microchim Acta

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A chromatographic system based on in-tube SPME coupled to capillary LC-DAD has been used to study the synthesis of silver nanoparticles using polyphenols in different scenarios: excess of the reducing agent or of the silver salt, addition of the cationic surfactants, and thermal synthesis. The optimized synthesis conditions allowed to quantify the polyphenols used as reducing agents, such as Trolox and chlorogenic acid. Two chromatographic peaks with different absorption spectrum were monitored during the syntheses. Depending on the molar relationship, a linear relation between the area of the chromatographic peaks and the concentration of the silver or polyphenol was established. For stabilization of silver nanoparticles, different cationic surfactants were used allowing to evaluate the role of anion (chloride and bromide) and of the alkyl chain. The proposed methodology can be used to determine chlorogenic acid up to 3 mM with a detection limit of 34 μM at λ= 400 nm. Chlorogenic acid was determined in dietary products with successful results. Precision (RSD=10%) and recovery (97–100%) were also satisfactory. Graphical Abstract

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Determination of antioxidant activity by in situ synthesis of AgNPs using in-tube SPME coupled on-line to capillary liquid chromatography

Prieto-Blanco,

Pardo-Puñal,

Moliner-Martínez

et al. 2023

Microchim Acta

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“…The method was modified by Soto et al (2022) . Ultra-high pressure liquid chromatography (UHPLC) was performed on an Agilent 1290 Infinity II LC system (Agilent, Santa Clara, CA, USA), which includes a quaternary solvent pump, an automatic injector, and a column oven.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of phytochemical profile, and antioxidant, antidiabetic activities of indigenous Thai fruits

Prakulanon,

Duangsrisai,

Vajrodaya

et al. 2024

PeerJ

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Background This research aims to explore the phenolics identification, phenolics quantification, antioxidant and potential biofunctional properties of lesser-known Thai fruits and their potency to treat type 2 diabetes mellitus (T2DM). Including, Antidesma puncticulatum, Dillenia indica, Diospyros decandra, Elaeagnus latifolia, Flacourtia indica, Garcinia dulcis, Lepisanthes fruticose, Mimusops elengi, Muntingia calabura, Phyllanthus reticulatus, Streblus asper, Syzygium cumini, Syzygium malaccense, Willughbeia edulis and Schleichera oleosa were analyzed by their phenolic and flavonoid content. These fruits have received limited scientific attention, prompting an investigation into their health benefits, particularly their relevance to diabetes management. Methods The study utilized methanolic crude extracts to measure phenolic and flavonoid levels. Additionally, UHPLC-DAD was utilized to identify and quantify phenolics. The methanolic extracts were assessed for antioxidant and antidiabetic abilities, including α-glucosidase and α-amylase inhibition. Results and Conclusion The study highlighted S. cumini as a rich source of phenolic (980.42 ± 0.89 mg GAE/g and flavonoid (3.55 ± 0.02 mg QE/g) compounds with strong antioxidant activity (IC50 by DPPH; 3.00 ± 0.01 µg/ml, IC50 by ABTS; 40 ± 0.01 µg/ml, FRAP; 898.63 ± 0.02 mg TE/ml). Additionally, S. cumini exhibited promising antidiabetic effects (S. cumini IC50; 0.13 ± 0.01 mg/ml for α-glucosidase inhibition, 3.91 ± 0.05 mg/ml for α-amylase inhibition), compared to Acarbose (IC50; 0.86 ± 0.01 mg/ml for α-glucosidase inhibition, 0.39 ± 0.05 mg/ml for α-amylase inhibition). Remarkably, compounds like catechins, gallic acid, kaempferol, and ellagic acid were identified in various quantities.This study suggests that these fruits, packed with phenolics, hold the potential to be included in an anti-diabetic diet and even pharmaceutical applications due to their health-promoting properties.

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Portable Liquid Chromatography

Cortés‐Bautista,

Campíns‐Falcó

2023

Encyclopedia of Analytical Chemistry

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The development of miniaturized systems has opened the possibility of the introduction of hand‐portable liquid chromatography ( LC ), but this kind of LC has evolved slowly although it has significant potential for solving analytical challenges in various applications in situ or at situ monitoring, due to their ability to perform measurements at the point of the potential analytical problem occurs. This article provides an overview of the current state of the art of portable LC technology and its context. The first LC reported as portable was the OB‐4 or Milichrom, developed by Baram et al. in 1983, but this system is far away from the contemporary idea of portability although served as a basis for the later developed portable LCs. It was not until 2015, after notable advancements in pumps, detectors, column technology, and batteries that a new wave of portable LCs surged. A timeline with relevant portable LC contributions is given and their main features are described in the article. The Axcend Focus was the first commercialized capillary/nano portable LC. Sharma et al. in 2015 proposed a number of requirements that an LC system must achieve to be considered as portable and Rahimi et al. in 2020 developed criteria to assess LC portability named portaBle fiEld Testing sTandard framEwoRk ( BETTER ) criteria, which evaluates: the system cost, performance, robustness, operation time, portability, and weight. To date, portable LC systems meet some of the grade 2 and 3 criteria for almost all assessments. The grade 4 and 5 criteria are deemed a challenge to be met by next‐generation devices. The capabilities of different pumping systems have been tested in several studies being the most popular option the syringe pumps. Injectors have not presented a major obstacle when they come to adapting to portable equipment. The downscaling of LC has been closely related to the dimensions of the analytical column, particularly to its internal diameter, capillary packed and monolithic columns have been employed. On‐column light‐emitting diode ( LED ) detection configurations, which do not make use of flow cells eliminating the associated dead volumes were proposed. Electrochemical detectors were also used in portable LC. Almost all portable LCs are homemade and were not commercialized, for most of them only a single paper was published and the number of real applications is scarce. New knowledge is necessary for advancing in portable LC.

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Hand-Portable Miniaturized Liquid Chromatography for the Determination of Chlorogenic Acids in Dietary Supplements

Cited by 4 publications

References 27 publications

Determination of antioxidant activity by in situ synthesis of AgNPs using in-tube SPME coupled on-line to capillary liquid chromatography

Determination of antioxidant activity by in situ synthesis of AgNPs using in-tube SPME coupled on-line to capillary liquid chromatography

Evaluation of phytochemical profile, and antioxidant, antidiabetic activities of indigenous Thai fruits

Portable Liquid Chromatography

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