The vibration perception threshold (VPT) is the minimum amplitude required for conscious vibration perception. VPT assessments are essential in medical diagnostics, safety, and human–machine interaction technologies. However, factors like age, health conditions, and external variables affect VPTs. Various methodologies and distinct procedures have been used to access VPTs, leading to challenges in establishing standardized protocols. Following the PRISMA methodology, this systematic review was conducted to answer the research question: “How are vibration perception thresholds assessed on the glabrous skin of the hands and fingers of healthy humans?” Searches were conducted across five databases to locate recent studies reporting data from VPT assessments, published in English, between 2012 and 2023. Thirty-nine studies met the inclusion criteria. Data on study goals and various methodological aspects was categorized and analyzed. Information gaps were identified, and this review offers recommendations for future studies to enhance standardization and facilitate data comparison. This review also suggests directions for future research, aiming to improve our understanding of how humans perceive haptic information.