Handbook of Biological Dyes and Stains

Sabnis, R. W.

doi:10.1002/9780470586242

Cited by 265 publications

(97 citation statements)

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“…In these assays, CT-DNA solutions are bound with ethidium bromide (EtBr), a compound known to exhibit fluorescence only when bound with DNA. 43 The addition of a competitively binding PC results in the displacement of bound EtBr and a subsequent loss in emission of the solution. Therefore, changes in emission upon the incremental addition of these competitive binders can be used to calculate K app .…”

Section: Dalton Transactions Papermentioning

confidence: 99%

Synthesis and analysis of the anticancer activity of platinum(ii) complexes incorporating dipyridoquinoxaline variants

Pages

Wormell

et al. 2014

Dalton Trans.

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Eight platinum(II) complexes with anticancer potential have been synthesised and characterised. These complexes are of the type [, where I L is either dipyrido[3,2-f:2',3'-h]quinoxaline (dpq) or 2,3-dimethyl-dpq (23Me 2 dpq) and A L is one of the R,R or S,S isomers of either 1,2-diaminocyclohexane (SS-dach or RR-dach) or 1,2-diaminocyclopentane (SS-dacp or RR-dacp). The CT-DNA binding of these complexes and a series of other complexes were assessed using fluorescent intercalator displacement assays, resulting in unexpected trends in DNA binding affinity. The cytotoxicity of the eight synthesised compounds was determined in the L1210 cell line; the most cytotoxic of these were [Pt(dpq)(SS-dach)]Cl 2 and [Pt(dpq)(RR-dach)]Cl 2 , with IC 50 values of 0.19 and 0.80 μM, respectively. The X-ray crystal structure of the complex [Pt(dpq)(SS-dach)](ClO 4 ) 2 ·1.75H 2 O is also reported.

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Section: Dalton Transactions Papermentioning

confidence: 99%

Synthesis and analysis of the anticancer activity of platinum(ii) complexes incorporating dipyridoquinoxaline variants

Pages

Wormell

et al. 2014

Dalton Trans.

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“…It absorbs light in UV range (UV absorbance maxima at 210 and 285 nm) and gives bright orange fluorescence in visible range with wavelength 605 nm (Sabnis 2010). Earlier it was being used as trypanocide medicine to treat trypanosomiasis in cattle (Stevenson et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

“…Other product such as EtBr Destroyer (Favorgen Biotech Corp), a chemical based product, claims to degrade and destroy EtBr as quickly as five minutes leading to non-fluorescent and non-mutagenic by-products. Removal of EtBr using activated charcoal and ion-exchange-resin and incineration are the recommended methods of ethidium bromide decontamination (Sabnis 2010;Saeidnia and Abdollahi, 2013; Sigma-Aldrich-MSDS).…”

Section: Introductionmentioning

confidence: 99%

Isolation of Ethidium Bromide Degrading Bacteria from Jharkhand

Kumar

Swarupa

Gandhi

et al. 2017

Int J Appl Sci Biotechnol

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Ethidium bromide (EtBr) is a carcinogenic and mutagenic agent which is widely used in research laboratories to probe nucleic acids by gel electrophoresis. It is generally buried underground (for solid waste) or disposed of pouring it down the sink (in case of liquid waste). Soil or drain microbial community may be able to take care of such substance else it will lead to contamination of our underground resources or others through defined and undefined routes. In view of the above assumption and literature reports the present study was undertaken to isolate and evaluate bacteria for removal, by bioaccumulation and /or biotransformation, of EtBr from contaminated sources and wastes, before their disposal to the environment. Two distinct bacteria both motile BR3 and BR4 could be identified from agarose-gel-waste containing 0.5-1.0 g/ml ethidium bromide. Both bacteria were found to grow on EtBr-NA plate (Nutrient-Agar supplemented with EtBr at a concentration of 30 g/ml) however only BR3 isolate showed large non-fluorescent-halo zone (characteristic to degradation of EtBr) when exposed to trans-UV light. Other isolate BR4 could accumulate EtBr within the colony biomass but did not show clear (non-fluorescent) hallow zone around it. However the bacterium was not able to utilize the EtBr as a sole carbon source.

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“…The pH dye chosen is eosin which is a fluorescent dye with the advantage of having a low fluorescent state in low pH conditions and higher fluorescence in higher pH conditions [pH>3.0, greater than the pKa (acid dissociation constant at logarithmic scale) of eosin]. It has an absorption maximum at 517 nm and an emission maximum at 538 nm [11].…”

Section: System Descriptionmentioning

confidence: 99%

A low-cost, portable optical sensing system with wireless communication compatible of real-time and remote detection of dissolved ammonia

et al. 2016

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Abstract:A low-cost and portable optical chemical sensor based ammonia sensing system that is capable of detecting dissolved ammonia up to 5 ppm is presented. In the system, an optical chemical sensor is designed and fabricated for sensing dissolved ammonia concentrations. The sensor uses eosin as the fluorescence dye which is immobilized on the glass substrate by a gas-permeable protection layer. A compact module is developed to hold the optical components, and a battery powered micro-controller system is designed to read out and process the data measured. The system operates without the requirement of laboratory instruments that makes it cost effective and highly portable. Moreover, the calculated results in the system can be transmitted to a PC wirelessly, which allows the remote and real-time monitoring of dissolved ammonia.

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Handbook of Biological Dyes and Stains

Cited by 265 publications

References 0 publications

Synthesis and analysis of the anticancer activity of platinum(ii) complexes incorporating dipyridoquinoxaline variants

Synthesis and analysis of the anticancer activity of platinum(ii) complexes incorporating dipyridoquinoxaline variants

Isolation of Ethidium Bromide Degrading Bacteria from Jharkhand

A low-cost, portable optical sensing system with wireless communication compatible of real-time and remote detection of dissolved ammonia

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