Handbook of Plant Ecophysiology Techniques

Roger, Manuel Joaquín Reigosa

doi:10.1007/0-306-48057-3

Cited by 71 publications

(3 citation statements)

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“…Intracellular polyamine content of S. agalactiae strains was visualized as described elsewhere [14, 27]. In brief, aliquots from bacterial cultures were pelleted.…”

Section: Methodsmentioning

confidence: 99%

Streptococcus agalactiae imports spermidine by a member of the amino acid/polyamine antiporter family to endure citric acid stress at the vaginal pH

et al. 2022

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Polyamines bind to various cellular components, such as nucleic acids, phospholipids, proteins and nucleotides. They are involved in the virulence and protection against physiological stresses of several bacterial species. Streptococcus agalactiae is able to colonize the vaginal tract of asymptomatic pregnant women and to resist, by an as yet poorly characterized mechanism, pH 4.0, the low physiological pH of this environment. We identified a transporter of the amino acid/polyamine antiporter family (SAK_1604 in strain A909) that shares 39.8 % similar amino acids with CadB and 34.7 % with PotE, two transporters implicated in acid resistance in Escherichia coli . We found that sak_1604 is overexpressed in the presence of spermidine and during citric acid stress at the vaginal pH, but not during lactic acid or HCl stresses at the same pH or during a sodium citrate stress at pH 7.4. Dihydrogen citrate is the predominant form of citric acid at pH 4.0. Using a deletion mutant, we proved that SAK_1604 is involved in the survival of S. agalactiae during citric acid stress at pH 4.0 in the presence of spermidine, and we showed by TLC analysis that it is involved in spermidine transport in these conditions. Our data open new perspectives on the comprehension of the molecular mechanisms allowing S. agalactiae to survive at the physiological pH of the vagina and on the unsuspected role of an ionic form of citric acid.

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“…Intracellular polyamine content of S. agalactiae strains was visualized as described elsewhere [14, 27]. In brief, aliquots from bacterial cultures were pelleted.…”

Section: Methodsmentioning

confidence: 99%

Streptococcus agalactiae imports spermidine by a member of the amino acid/polyamine antiporter family to endure citric acid stress at the vaginal pH

et al. 2022

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“…Las muestras se almacenaron en una hielera para determinar: A) el potencial hídrico (Ψ) mediante un potenciómetro de punto de rocío WP4C (Decagon Devices, Inc., Washington, USA), este parámetro se midió en menos de 24 horas después de la colecta, y B) el contenido relativo de agua (CRA), para lo cual las muestras fueron pesadas (peso fresco), sumergidas en agua destilada hasta que el peso no varió (48 horas) y pesadas de nuevo (peso a saturación), y secadas en un secador artesanal a 60 ºC por 48 horas, después de lo cual se pesaron de nuevo (peso seco). El CRA se calculó siguiendo a González & González-Vilar (2001) como: (4)…”

Section: Materiales Y Métodosunclassified

Respuesta fisiológica del maíz en monocultivo y milpa en suelos superficiales y profundos de Yucatán, México

Mares-Guerrero,

Cach-Pérez,

Reyes-García

et al. 2023

Bot. Sci.

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Antecedentes: El manejo agrícola modifica el microclima en el área de cultivo, afectando la fisiología y productividad de las plantas. Preguntas: ¿Cómo varía el microclima, fisiología y productividad del maíz en un monocultivo (M) y una milpa (policultivo, MFC) en suelos profundo y superficial de Yucatán? Especie de estudio: Zea mays L. Sitio de estudio: Yucatán, México; 2022 Métodos: Se midió temperatura del aire y suelo, déficit de presión de vapor, formación de rocío y contenido volumétrico de agua en suelo (CVA); asimilación de CO2 (A), transpiración (E), conductancia estomática (gs), eficiencia en el uso del agua, contenido de clorofila (CC), eficiencia cuántica, tasa de transporte de electrones y disipación no fotoquímica en plantas de maíz a 40 y 75 días después de la siembra, en milpas y monocultivos en Peto (suelo superficial, alta pedregosidad) y Mama (suelo más profundo, baja pedregosidad), Yucatán. Resultados: La temperatura del suelo fue hasta 5 °C mayor en Peto y 2 °C en Mama en M respecto a MFC, que registró hasta 22 % mayor CVA que M. El maíz en Mama presentó mayor A y E que en Peto; las plantas en MFC en Peto tuvieron mayor A, E y gs que las de M; en Mama fue lo contrario. El CRA y CC fue mayor en plantas de MFC en Peto. Conclusiones: La modificación microclimática y su efecto sobre el maíz dependió del suelo: MFC fue más productiva en suelos superficiales, mientras M en suelos profundos. El CVA fue mayor en MFC.

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“…The achieved results were expressed as µM Trolox equivalent (TE) of tomato fruit fresh weight using a standard curve with R 2 = 0.98. The obtained FRAP results were expressed in µM TE/g fresh weight and then calculated using the formula below (Equation ( 5 The extraction and analysis of protein were carried out using the combined techniques of Jumnongpon; Raseetha and Bonjoch [24][25][26], with minor modifications. The chemicals used to extract and evaluate enzymes were of analytical grade.…”

Section: Ferric Reducing Antioxidant Powermentioning

confidence: 99%

Controlling Fusarium oxysporum Tomato Fruit Rot under Tropical Condition Using Both Chitosan and Vanillin

et al. 2021

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Tomato Lycopersicon esculentum Mill. is one of the most cultivated and widely consumed vegetables in the world. However, it is very susceptible to the infection initiated by Fusariumoxysporum fruit rot, which shortens post-harvest life and thus reduces market value. This disease can be regulated appropriately by the application of synthetic fungicides. However, chemical fungicides constitute a serious health risk, and have harmful environment effects and increase disease resistance, even when microbes are dead. Hence, to overcome this problem, chitosan and vanillin, which have antimicrobial bioactive properties against the growth of microorganisms, could be an alternative to disease control, while maintaining fruit quality and prolonging shelf life. The aim of this research was to evaluate the antimicrobial activity of chitosan and vanillin towards the inoculate pathogen and to investigate the effect of chitosan and vanillin coating in vivo on Fusarium oxysporum fruit rot and defense-related enzymes (PAL, PPO and POD). Chitosan and vanillin in aqueous solutions, i.e., 0.5% chitosan + 10 mM vanillin, 1% chitosan + 10 mM vanillin, 1.5% chitosan + 10 mM vanillin, 0.5% chitosan + 15 mM vanillin, 1% chitosan + 15 mM vanillin and 1.5% chitosan + 15 mM vanillin, were used as edible coatings on tomatoes stored at 26 ± 2 °C and 60 ± 5 relative humidity. The result revealed 1.5% chitosan + 15 mM vanillin was able to control disease incidence by 70.84% and severity by 70%. These combinations of coatings were also able to retain phenylalanine ammonia-lyase (PAL), peroxidase activity (POD), and polyphenol oxidase (PPO) enzyme activities as well as prolong shelf life of tomatoes up to 15 days.

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Handbook of Plant Ecophysiology Techniques

Cited by 71 publications

References 0 publications

Streptococcus agalactiae imports spermidine by a member of the amino acid/polyamine antiporter family to endure citric acid stress at the vaginal pH

Streptococcus agalactiae imports spermidine by a member of the amino acid/polyamine antiporter family to endure citric acid stress at the vaginal pH

Respuesta fisiológica del maíz en monocultivo y milpa en suelos superficiales y profundos de Yucatán, México

Controlling Fusarium oxysporum Tomato Fruit Rot under Tropical Condition Using Both Chitosan and Vanillin

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