2016
DOI: 10.1038/am.2016.70
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Handheld energy-efficient magneto-optical real-time quantitative PCR device for target DNA enrichment and quantification

Abstract: A PCR provides not only valuable genetic information that enables precise differential disease diagnosis but also quantitative data to assess various clinical states. We report the successful integration of novel dual-mode magnetic Fe 3 O 4 nanoclusters that deliver photothermal conversion. The clusters can be excited with pulsed laser light for precision thermal cycle modulation to develop an ultrafast quantitative PCR system. Traditional PCR heats and cools the sample from outside the PCR tube; the heat need… Show more

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Cited by 36 publications
(19 citation statements)
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“…Most polymerase chain reaction methods rely on thermal heating and cooling cycles to induce DNA melting and enzyme-driven DNA replication. The treatment solution volume is normally small and is particularly suitable for MOFC reactor processing [207][208][209][210][211][212][213][214][215][216][217]. Plasmonic light-to-heat energy conversion contributed to a breakthrough ultrashort polymerase chain reaction cycle time of 30 cycles in 54 seconds [218].…”
Section: Plasmonic Light-to-heat Conversionmentioning
confidence: 99%
“…Most polymerase chain reaction methods rely on thermal heating and cooling cycles to induce DNA melting and enzyme-driven DNA replication. The treatment solution volume is normally small and is particularly suitable for MOFC reactor processing [207][208][209][210][211][212][213][214][215][216][217]. Plasmonic light-to-heat energy conversion contributed to a breakthrough ultrashort polymerase chain reaction cycle time of 30 cycles in 54 seconds [218].…”
Section: Plasmonic Light-to-heat Conversionmentioning
confidence: 99%
“…Although faecal sampling has been a routine activity of the national control programme for bilharzia and intestinal worms for over a decade [48, 49], an immediate drawback in the use of EPF sampling is the need for a laboratory setting. Future advances in handheld rtPCR equipment may provide an alternative avenue by which EPF sampling becomes more portable and affordable in field conditions [7, 50, 51]. What is evident from the results of this study is that EPF sampling of Plasmodium infections in school children in this high transmission setting provides information without invasive sampling.…”
Section: Discussionmentioning
confidence: 85%
“…The PCR efficiency of this device could be reached within the range of 90%-110%, and the limit of detection was 10 copies per reaction. Li et al [342] developed a photonic-PCR system that integrated Fe 3 O 4 nanoclusters for effective photothermal cycle modulation with a battery-powered device for successful data transfer (Figure 13(b)). The system was energy efficient and easy establishment, which efficaciously applied for the detection of defective mtDNA in mitochondrial diseases with low initial template concentration (50 pg-500 ng) and highly rea- sonable linearity.…”
Section: Pcr Devicementioning
confidence: 99%
“…(a) Schematic illustration of the portable real-time PCR device with continuous flow thermal cycling for multiple DNA sample analysis[341], Copyright 2014 Royal Society of Chemistry. (b) Schematic illustration of the handheld qPCR device integrated with magnetic nanoparticles for effective target DNA detection[342], Copyright 2016 Nature Publishing Group. (c) Schematic illustration of the adaptive PCR device to dynamically control and monitor the DNA replication processes[343], Copyright 2017 American Chemical Society (color online).…”
mentioning
confidence: 99%