Handmade Cloned Buffalo (<i>Bubalus bubalis</i>) Embryos Produced from Somatic Cells Isolated from Milk and Ear Skin Differ in Their Developmental Competence, Epigenetic Status, and Gene Expression

Jyotsana, Basanti; Sahare, Amol Ashok; Raja, A; Singh, Kiran; Singla, S. K.; Chauhan, M. S.; Manik, R. S.; Palta, P.

doi:10.1089/cell.2015.0027

Cited by 7 publications

(2 citation statements)

References 33 publications

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“…Successful isolation and culture of neutrophils, lymphocytes, and macrophages from colostrum and milk from buffaloes have been developed [ 85 , 125 ]. Milk-derived cells have been used for producing handmade cloned embryos of quality similar to that of skin-derived embryos although with a lower blastocyst rate [ 126 ]. Furthermore, when the RNA quality is conserved, mammary epithelial cells isolated from milk are a valuable, non-invasive source of mammary mRNA to study various factors that impact milk yield and composition, namely, once daily milking, feeding level, endocrine status, photoperiodic modulation, and stage of lactation [ 127 ].…”

Section: Future Use Of Milk Sccmentioning

confidence: 99%

Milk somatic cells, factors influencing their release, future prospects, and practical utility in dairy animals: An overview

2018

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Milk somatic cells (SCs) are a mixture of milk-producing cells and immune cells. These cells are secreted in milk during the normal course of milking and are used as an index for estimating mammary health and milk quality of dairy animals worldwide. Milk SC is influenced by cow productivity, health, parity, lactation stage, and breed of an animal. Any change in environmental conditions, poor management practices, and also stressful conditions significantly increases the amount of SC coming in milk. Better hygiene and proper nutrition help in reducing milk SC. Milk with low SC means better milk products with a longer shelf life. The present review describes the role of SCs (both secretory and immune) in milk, their role in maintaining the integrity of the mammary gland, and factors affecting their release in milk. This information may help to reduce milk somatic cell counts (SCCs) and to establish differential SCC standards.

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Section: Future Use Of Milk Sccmentioning

confidence: 99%

Milk somatic cells, factors influencing their release, future prospects, and practical utility in dairy animals: An overview

2018

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“…Additionally, we have circumstantial evidence, through the presence of what was morphologically an embyroid body ( S1 Fig ) that iPSCs can be generated from urine directly, as has previously been done with human urine, further validating its potential across species. This is, also to our knowledge, the first report of cloned cattle embryos produced from UDCs and; the only other report of cloning using UDCs was in the production of a buffalo ( Bubalus bubalis ) calf, by means of handmade cloning (HMC) from cells isolated from urine and from milk [ 26 , 27 ]. In this report they noted significant difference in genes related to histone modifications of H3K27me3 and cellular differentiation and to have a potential regulatory capacity in stem cells [ 28 , 29 ].…”

Section: Discussionmentioning

confidence: 99%

Live births from urine derived cells

Olsson¹,

Yeonwoo²,

Park

et al. 2023

PLoS ONE

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Here we report urine-derived cell (UDC) culture and subsequent use for cloning which resulted in the successful development of cloned canine pups, which have remained healthy into adulthood. Bovine UDCs were used in vitro to establish comparative differences between cell sources. UDCs were chosen as a readily available and noninvasive source for obtaining cells. We analyzed the viability of cells stored in urine over time and could consistently culture cells which had remained in urine for 48hrs. Cells were shown to be viable and capable of being transfected with plasmids. Although primarily of epithelial origin, cells were found from multiple lineages, indicating that they enter the urine from more than one source. Held in urine, at 4°C, the majority of cells maintained their membrane integrity for several days. When compared to in vitro fertilization (IVF) derived embryos or those from traditional SCNT, UDC derived embryos did not differ in total cell number or in the number of DNA breaks, measured by TUNEL stain. These results indicate that viable cells can be obtained from multiple species’ urine, capable of being used to produce live offspring at a comparable rate to other cell sources, evidenced by a 25% pregnancy rate and 2 live births with no losses in the canine UDC cloning trial. This represents a noninvasive means to recover the breeding capacity of genetically important or infertile animals. Obtaining cells in this way may provide source material for human and animal studies where cells are utilized.

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