Handyfuge Microfluidic for On-Site Antibiotic Susceptibility Testing

Abstract

Low-cost, rapid, and accurate acquisition of minimum inhibitory concentrations (MICs) is key to limiting the development of antimicrobial resistance (AMR). Until now, conventional antibiotic susceptibility testing (AST) methods are typically time-consuming, high-cost, and labor-intensive, making them difficult to accomplish this task. Herein, an electricity-free, portable, and robust handyfuge microfluidic chip was developed for on-site AST, termed handyfuge-AST. With simply handheld centrifugation, the bacterial-antibiotic mixtures with accurate antibiotic concentration gradients could be generated in less than 5 min. The accurate MIC values of single antibiotics (including ampicillin, kanamycin, and chloramphenicol) or their combinations against Escherichia coli could be obtained within 5 h. To further meet the growing demands of point-of-care testing, we upgraded our handyfuge-AST with a pH-based colorimetric strategy, enabling naked eye recognition or intelligent recognition with a homemade mobile app. Through a comparative study of 60 clinical data (10 clinical samples corresponding to six commonly used antibiotics), the accurate MICs by handyfuge-AST with 100% categorical agreements were achieved compared to clinical standard methods (area under curves, AUCs = 1.00). The handyfuge-AST could be used as a low-cost, portable, and robust point-of-care device to rapidly obtain accurate MIC values, which significantly limit the progress of AMR.

Centrifugo-Pneumatic Reciprocating Flowing Coupled with a Spatial Confinement Strategy for an Ultrafast Multiplexed Immunoassay

“…Alternatively, centrifugal microfluidics, commonly referred to as lab-on-a-disc systems (LOAD), , has received considerable recognition within academic and industrial spheres owing to its capacity for comprehensive automation, diminished cross-contamination, elimination of external pumps, and proven effectiveness in a sample-in-result-out or point-of-care immunoassay. ,− To enhance the probability of interaction, recently, our group have also demonstrated an innovative active centrifugal microfluidic chip for a fully automated immunoassay . However, its utilization of complicated dual active online valve control technology has restrained the POCT potential of the immunoassay.…”

Multiple on-line active valves based centrifugal microfluidics for dynamic solid-phase enrichment and purification of viral nucleic acid

“…13–16 This innovation arranges microfluidic channels and reaction chambers on a rotating disk, harnessing the mechanical effects of centrifugal force, Coriolis force, and Euler force generated during disk rotation. 17–21 This enables precise manipulation and operation of samples and reagents. 22–30…”

“…[13][14][15][16] This innovation arranges microfluidic channels and reaction chambers on a rotating disk, harnessing the mechanical effects of centrifugal force, Coriolis force, and Euler force generated during disk rotation. [17][18][19][20][21] This enables precise manipulation and operation of samples and reagents. [22][23][24][25][26][27][28][29][30] Valve control on centrifugal microfluidic chips stands as a pivotal technology in this field, typically classified into two categories: passive and active valves.…”

Pt/Zn-TCPP Nanozyme-Based Flexible Immunoassay for Dual-Mode Pressure–Temperature Monitoring of Low-Abundance Proteins

“…With the rapid development of bionanotechnologies, new biosensing and fabrication techniques are being used to design portable instruments. , Compared with traditional detection methods using large-scale instruments like fluorescence and mass spectrometry, portable detection has the advantages of low cost, simple instrumentation, flexibility, and easy operation. − These attributes render portable detection highly promising for on-site or on-spot screening across diverse applications . Flexible sensors, characterized by their compatibility with movable components and arbitrarily curved surfaces, are widely used in wearable devices, health monitoring instruments, robots, and other fields .…”