This study involved the collection of juvenile fall Chinook salmon and Columbia River water samples to assess whether fall Chinook salmon were exposed to chromium that upwells into the river from contaminated groundwater originating at the Hanford Site. Juvenile fall Chinook salmon were seined and water samples were collected from three locations in the Hanford Reach during the period of juvenile salmon freshwater residency in early May, mid-May, and mid-June 2002. The concentrations of chromium in fish and river water were measured and the overall conditions of the fish were evaluated. Sample collection focused on the 100-D and 100-H Areas, which were the locations most likely to have elevated chromium concentrations in the environment based on groundwater monitoring data. The Vernita Bridge area served as an upstream reference site for all samples. All Columbia River water concentrations for chromium determined during this study were less than or equal to 0.1 µg/L, which were below the Washington State ambient surface-water quality criteria of 10 µg/L. Body burdens of chromium were not statistically different for fish collected at the 100-D and 100-H Areas compared to the Vernita Bridge location; thus, there was no indication of elevated exposure or uptake of Hanford sources of chromium. No gross morphological anomalies were noted in any fish collected during this effort. Histological assessments for fish examined during this study exhibited normal and healthy tissues and comparison of fish body lengths and weights from these locations revealed no evidence of physiological stress for organisms collected near the 100-D or 100-H Areas. Taken collectively, these results indicated that there was no impact to juvenile fall Chinook salmon from chromium released into the Columbia River from Hanford during 2002. v