Hanultarin, a cytotoxic lignan as an inhibitor of actin cytoskeleton polymerization from the seeds of Trichosanthes kirilowii

Moon, Surk Sik; Rahman, Aziz Abdur; Kim, Joo Young; Kee, Sun Ho

doi:10.1016/j.bmc.2008.06.032

Cited by 67 publications

(50 citation statements)

References 37 publications

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“…T. kirilowii, a well known folk herb of East Asia, has been reported to possess many bioactive components, such as trichosanic acid, trichosanthin, trichosans, diterpene and triterpenoids. 25 However, no alkaloids, such as ajmalicine, serpentine, vindoline and catharanthine, were reported in T. kirilowii, which was also confirmed in this study. As shown in Fig.…”

Section: Detection and Selectivity Of The Alkaloids By Uplc-mssupporting

confidence: 86%

Simultaneous Quantification of Four Indole Alkaloids in Catharanthus roseus Cell Line C20hi by UPLC-MS

Yang

Xiong

et al. 2011

ANAL. SCI.

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An ultra-performance liquid chromatography/mass spectrometry method to simultaneously quantify vindoline, catharanthine, serpentine and ajmalicine in Catharanthus roseus cell line C20hi is reported. Samples were extracted with 1% acetic acid, basified to pH 10 with ammonia, then extracted with ethyl acetate, dried, reconstituted with methanol-1% acetic acid water solution (1:1, v/v) and analyzed using an acetonitrile-0.1% formic acid gradient as the mobile phase. Detection was carried out by electrospray ionization mass spectrometry in the positive-ion mode with selective ion monitoring. The analysis of one sample was achieved in 6 min. The limits of detection were 0.46 -0.70 ng/ml in cell samples, and 0.10 -0.16 ng/ml in medium samples. The linearity of detection was over the wide range of 1.00 -6250.0 ng/ml. Intra-and inter-day accuracies (recovery 88.0 -111.8%) and precision (RSD 1.25 -7.81%) showed the performance of the assay. This method provides a more sensitive and high-throughput technique to quantify the four alkaloids in large amount of samples, and will be helpful in high-production cultivar screening.

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Section: Detection and Selectivity Of The Alkaloids By Uplc-mssupporting

confidence: 86%

Simultaneous Quantification of Four Indole Alkaloids in Catharanthus roseus Cell Line C20hi by UPLC-MS

Yang

Xiong

et al. 2011

ANAL. SCI.

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“…It has been reported that extracts from Trichosanthes kirilowii, such as liganan, trichosanthin and chitinase, have positive effects against cancer through preventing or inhibiting the process of carcinogenesis (Moon et al, 2008;Xu et al, 2008). A pharmacogenomic analysis of a cancer chemopreventive formulation needs to be considered, and the composition of the formulation should improve over time.…”

Section: Discussionmentioning

confidence: 99%

The mechanisms of action of Tianhua^™on antitumor activity in lung cancer cells

Lin

Kao

et al. 2010

Pharmaceutical Biology

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“…25 However, there are no reports on the effect of (-) catechin on ethanol metabolism. Isolates 1 and 2 did not show cytotoxicity below 100 µg/mL against cancer cell lines (small lung carcinoma A549), 26,27 DPPH-radical scavenging activity, 28 or antimicrobial activities (Staphylococcus aureus and Candida albicans). 27,29 The results presented suggest that the isolates could perform as effective ADH inhibitors to alleviate the formation of acetaldehyde during the oxidation process of ethanol metabolism and hence reduce the possibility of hangover symptoms.…”

Section: Resultsmentioning

confidence: 99%

Gamnamoside, a Phenylpropanoid Glycoside from Persimmon Leaves (Diospyros kaki) with an Inhibitory Effect against an Alcohol Metabolizing Enzyme

2009

Bulletin of the Korean Chemical Society

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Phytochemical investigation of the methanolic extract of Diospyros kaki leaves led to the isolation of osmanthuside H (1) and a new phenol glycoside, named gamnamoside [4-(3-hydroxypropyl)-2-methoxyphenol β-D-apiofuranosyl(1 → 6)β-D-glucopyranoside] (2) along with (-) catechin (3) through a series of reversed phase column chromatography and preparative C18 HPLC. The structures of the isolates were determined by spectroscopic methods including IR, UV, HRTOFMS, and 2D NMR. Compounds 1, 2, and 3, showed good inhibitory activities (IC50) of 175.4, 94.4, and 126.6 µg/mL respectively, whereas a reversible ADH inhibitor, 4-methylpyrazole, showed the IC50 of 326.6 µg/mL against alcohol dehydrogenase (ADH).Key Words: Persimmon, Diospyros kaki, Gamnamoside, (-) Catechin, Alcohol dehydrogenase

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Hanultarin, a cytotoxic lignan as an inhibitor of actin cytoskeleton polymerization from the seeds of Trichosanthes kirilowii

Cited by 67 publications

References 37 publications

Simultaneous Quantification of Four Indole Alkaloids in Catharanthus roseus Cell Line C20hi by UPLC-MS

Simultaneous Quantification of Four Indole Alkaloids in Catharanthus roseus Cell Line C20hi by UPLC-MS

The mechanisms of action of Tianhua^™on antitumor activity in lung cancer cells

Gamnamoside, a Phenylpropanoid Glycoside from Persimmon Leaves (Diospyros kaki) with an Inhibitory Effect against an Alcohol Metabolizing Enzyme

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Hanultarin, a cytotoxic lignan as an inhibitor of actin cytoskeleton polymerization from the seeds of Trichosanthes kirilowii

Cited by 67 publications

References 37 publications

Simultaneous Quantification of Four Indole Alkaloids in Catharanthus roseus Cell Line C20hi by UPLC-MS

Simultaneous Quantification of Four Indole Alkaloids in Catharanthus roseus Cell Line C20hi by UPLC-MS

The mechanisms of action of Tianhua™on antitumor activity in lung cancer cells

Gamnamoside, a Phenylpropanoid Glycoside from Persimmon Leaves (Diospyros kaki) with an Inhibitory Effect against an Alcohol Metabolizing Enzyme

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The mechanisms of action of Tianhua^™on antitumor activity in lung cancer cells