Haploids

Reinert, J.; Bajaj, Y. P. S.; Nitsch, Colette; Clapham, David; Jensen, C. J.

doi:10.1007/978-3-662-02279-5_2

Cited by 7 publications

(6 citation statements)

References 157 publications

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“…According to Santoso [35], the difference in endogenous hormones in each explant, even when cultured in the same culture medium, can affect its response to the addition of growth regulators. The relationship between the growth regulators (exogenous) and plant endogenous substances will increase the growth and morphogenesis of the culture [24,36]. Murni [37] reported that seeds contain growth regulators such as auxins, cytokinins, gibberellins, and so on, which help in the growth and development of the seedling under in vitro as well as in vivo conditions.…”

Section: Discussionmentioning

confidence: 99%

Plant Growth Hormones and Micro-Tuberization in Breaking the Seed Dormancy of Bunium persicum (Boiss.) Fedts

Khan,

Dar,

Alie

et al. 2023

Plants

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Bunium persicum is a valuable medicinal plant with limited production but high market demand. It thrives predominantly in high-altitude regions. The main challenges hindering its widespread cultivation are seed dormancy and a lengthy seed-to-seed cycle, making its large-scale cultivation difficult. Six genotypes of Bunium persicum were collected from different altitudes to evaluate its germination behavior and seed dormancy. The study was conducted during 2020–23 and comprised three experiments (viz., seed germination under an open field, controlled conditions, and micro-tuberization). Under open field conditions, germination percent was genotype dependent, and the highest germination percentage, root length, and shoot length were recorded in Shalimar Kalazeera-1. Germination behavior assessment of the Bunium persicum revealed that treatment T9 (GA3 (25 ppm) + TDZ (9 µM/L)) is effective in breaking the dormancy of Bunium persicum as well as in obtaining a higher germination percent for early development of the tubers. Similarly, with regard to the effect of temperature and moisture conditions, stratification under moist chilling conditions showed effectiveness in breaking seed dormancy as the germination percentage in stratified seeds was at par with the most efficient growth hormone. With regard to the in vitro micro-propagation, direct regeneration showed multiple shoot primordia at the base of the tubers without intervening callus phase from the MS medium supplemented with BA (22.2 µM) and NAA (13.95 µM) 4 weeks after sub-culturing. Similarly, medium supplemented with JA (8.0 mg/L) and BA (22.2 µM) produced well-organized somatic embryos with shiny surfaces, which appeared at the swelled basal portion of apical stems. Further, the combination of JA (6.0 mg/L) and BA (22.2 M) was effective in developing the micro-tubers and also enhanced the weight and length of Bunium persicum micro-tubers.

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Section: Discussionmentioning

confidence: 99%

Plant Growth Hormones and Micro-Tuberization in Breaking the Seed Dormancy of Bunium persicum (Boiss.) Fedts

Khan,

Dar,

Alie

et al. 2023

Plants

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“…Sukrosa adalah karbohidrat translokasi utama dalam jaringan tanaman, dan merupakan sumber karbon yang paling umum digunakan dalam kultur anthera, biasanya pada tingkat 2-4% (Reinert & Bajaj, 1977). Tingkat sukrosa yang tinggi (6-17%) diperlukan pada spesies tertentu (misalnya Gramineae, Cruciferae) dimana serbuk sari matang dalam kondisi triseluler (Dunwell & Thurling, 1985), sedangkan pada spesies Solanaceae yang serbuk sarinya matang berbentuk biseluler, memerlukan sukrosa pada tingkat yang lebih rendah, seperti 2-5% (Dunwell, 2010).…”

Section: Media Pertumbuhanunclassified

Kultur Anther dalam Produksi Tanaman Haploid: Review

Apriliani

2024

Agiotech

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Tanaman haploid adalah tumbuhan dengan jumlah kromosom gametofit dan double haploid adalah tanaman haploid yang telah mengalami penggandaan kromosom. Produksi tanaman haploid dan double haploid dilakukan melalui embriogenesis gamet memungkinkan perkembangan suatu galur homozigot dari tetua heterozigot, sehingga mempersingkat waktu yang diperlukan untuk menghasilkan tanaman homozigot dibandingkan dengan metode pemuliaan konvensional yang menggunakan beberapa generasi. Adanya kemajuan dalam bidang bioteknologi memberikan dampak kemajuan yang signifikan pada sektor perrtanian. Termasuk telah ditemukan beberapa metode yang untuk memproduksi tanaman haploid dan double haploid, seperti kultur anther in vitro atau mikrospora yang paling efektif dan banyak digunakan oleh para pemulia tanaman. Kultur anther memiliki potensi yang tinggi, namun perlu adanya persiapan yang matang dalam pelaksanaan terkait dengan protokol, sumber eksplan, pre-treatment, kondisi lingkungan, serta media pertumbuhan.

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“…The culture media needs carbohydrate source. Sucrose is commonly utilized for these purposes but maltose has supremacy in a few contexts compared to sucrose, such as albino plant frequency reduced by maltose utilization (Reinert and Bajaj 1977;Sen et al 2011;Park et al 2013). Besides these -sorbitol, amino acid, and AgNO 3 were found beneficial for plant regeneration from androgenesis in rice (Yoshida et al 1994;Ogawa et al 1995;Faruq et al 2014).…”

Section: Rice Perspectivesmentioning

confidence: 99%

Anther Culture in Crop Plants: Progress and Perspectives

Islam

Arif

Hasan

et al. 2023

Plant Breed. Biotech.

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A resurrection has started in haploid and double haploid research in the twenty-first century. The haploid and double haploid could be achieved through in vivo and in vitro anther and microspore culture techniques. Fixing the homozygosity is the most striking benefit of androgenesis. Various factors like genotypic dependency, growth condition, developmental stage of the microspore, pre-treatment, culture media, regeneration media, growth hormones, and various chemicals have a direct effect. Wheat, rice, Brassica, and tobacco are the notable crops where anther and microspore culture has been utilized. These haploidy and double haploidy through anther culture served many purposes of basic and applied research. Especially, double haploid cultivars have been cultivating around the globe. In addition, for chromosome mapping, QTL mapping, marker-assisted selection, marker-assisted backcrossing, mutation breeding, genome-wide association study, genomic engineering, and genome editing, androgenesis based haploid and double haploid plants have been exploited due to the effectiveness. Recently, researchers are trying to explain albinism that happens during anther culture from an epigenetic perspective. Further prospects of haploid and doubled haploid research through anther culture have been described in this review.

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Haploids

Cited by 7 publications

References 157 publications

Plant Growth Hormones and Micro-Tuberization in Breaking the Seed Dormancy of Bunium persicum (Boiss.) Fedts

Plant Growth Hormones and Micro-Tuberization in Breaking the Seed Dormancy of Bunium persicum (Boiss.) Fedts

Kultur Anther dalam Produksi Tanaman Haploid: Review

Anther Culture in Crop Plants: Progress and Perspectives

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