Haploidy in Cultivated Wheats: Induction and Utility in Basic and Applied Research

Jauhar, Prem P.; Xu, Steven S.; Baenziger, P. Stephen

doi:10.2135/cropsci2008.08.0462

Cited by 60 publications

(53 citation statements)

References 144 publications

(265 reference statements)

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“…With DH production systems established, homozygous lines can be achieved in one generation, which would allow to reduce the time and cost of cultivar development (Dunwell 2010;Ferrie and Caswell 2010;Germanà 2011). The system has been effectively used in barley, wheat (Jauhar et al 2009;Ferrie and Caswell 2010), maize (Smith et al 2008;Chang and Coe 2009), and various Brassica species (Belmonte et al 2006;Fang et al 2006;Zhang et al 2008;Takahira et al 2011;Winarto and Teixeira da Silva 2011). The genotype is one of the crucial factors evaluated in many plants (Ferrie et al 1995;Dunwell 2010).…”

Section: Discussionmentioning

confidence: 99%

Microspore embryogenesis and production of haploid and doubled haploid plants in carrot (Daucus carota L.)

Zhuang

et al. 2012

Plant Cell Tiss Organ Cult

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Protocols were developed for the generation of haploid and doubled haploid plants from isolated microspores of carrot (Daucus carota L.). Forty-seven carrot accessions, including six inbred lines, 11 cultivars, 20 F 1 s, two BC 1 F 1 s, four F 2 s, one F 3 , and three F 4 s, were screened to evaluate the genotype influence on isolated microspore embryogenesis over 4 years. Twenty-eight accessions responded by producing embryos and/or calli. A cytological analysis showed that two modes of carrot microspore embryogenesis exist: an indirect route via calli (C mode), and a direct route via embryos (E mode). Eleven accessions were in the C mode, and 17 were in both modes. The highest production rates were in 10Y25 (a European Nantes cultivar) with 27 calli and 307 embryos, and 100Q6 (a semi-Nantes F 1 hybrid) with 176 calli and 114 embryos. The time period to produce embryos or calli differed significantly between 2 and 6 months. Cold and heat pretreatment generally had a negative impact on the induction of microspore embryogenesis, but a short pretreatment showed a positive influence on some accessions. Twentyeight lines regenerated plants from the primary individual embryos or calli of three accessions were established to analyze the ploidy level. The percentage of spontaneous diploidization showed very wide differences among the accessions and lines. Differences in leaf color intensity, leaf size, and leaf dissection were found among haploid, doubled haploid, and triploid plants.

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Section: Discussionmentioning

confidence: 99%

Microspore embryogenesis and production of haploid and doubled haploid plants in carrot (Daucus carota L.)

Zhuang

et al. 2012

Plant Cell Tiss Organ Cult

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“…Ali used 223 CNN(RICL3A)s developed using the monosomic method (Law 1966;Yen & Baenziger 1992) and doubled haploidy (Lizarazu et al 1992;Jauhar et al 2009) and 32 markers to precisely map the QTLs identified by Campbell et al (2003). Phenotypic data was collected from 5 to 6 environments using four replications.…”

Section: Current Researchmentioning

confidence: 99%

Understanding grain yield: it is a journey, not a destination

Baenziger

Dweikat

Gill

et al. 2011

CZECH J GENET PLANT

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Abstract:Approximately 20 years ago, we began our efforts to understand grain yield in winter wheat using chromosome substitution lines between Cheyenne (CNN) and Wichita (WI). We found that two chromosome substitutions, 3A and 6A, greatly affected grain yield. CNN(WI3A) and CNN(WI6A) had 15 to 20% higher grain yield than CNN, whereas WI(CNN3A) and WI(CNN6A) had 15 to 20% lower grain yield than WI. The differences in grain yield are mainly expressed in higher yielding environments (e.g. eastern Nebraska) indicating genotype by environment interactions (G × E). In studies using hybrid wheat, the gene action for grain yield on these chromosomes was found to be mainly controlled by additive gene action. In subsequent studies, we developed recombinant inbred chromosome lines (RICLs) using monosomics or doubled haploids. In extensive studies we found that two regions on 3A affect grain yield in the CNN(RICLs-3A) with the positive QTLs coming from WI. In WI(RICLs-3A), we found a main region on 3A that affected grain yield with the negative QTL coming from CNN. The 3A region identified using WI(RICLs-3A) coincided with one of the regions previously identified in CNN(RICLs-3A). As expected the QTLs have their greatest effect in higher-yielding environments and also exhibit QTL × E. Using molecular markers on chromosomes 3A and 6A, the favorable alleles on 3A in Wichita may be from Turkey Red, the original hard red winter wheat in the Great Plains and presumably the original source of the favorable alleles. Cheyenne, a selection from Crimea, did not have the favorable alleles. In studying modern cultivars, many high yielding cultivars adapted to eastern Nebraska have the WI-allele indicating that it was selected for in breeding higher yielding cultivars. However, some modern cultivars adapted to western Nebraska where the QTL has less effect retain the CNN-allele, presumably because the allele has less effect (is less important in improving grain yield). In addition many modern cultivars have neither the WI-allele, nor the CNN-allele indicating we have diversified our germplasm and new alleles have been brought into the breeding program in this region.

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“…Haploidy offers an efficient method for producing truly homozygous lines, thus accelerating plant breeding. DH-derived wheat cultivars have been released elsewhere (Jauhar et al 2009). Interspecific hybridization allows the introgression of diversity from wheat's wild relatives, thus bringing genes with large or small effects not available in the wheat cultigen pool.…”

Section: Evolution and Diversitymentioning

confidence: 99%

Breeding Self-Fertilizing Plants: From Inbred to Hybrid Cultivars

Ríos

2015

Plant Breeding in the Omics Era

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Pollen falls on to the stigma of the same flower in self-fertilizing (selfing) species, which are often annuals with little plant height, small flowers, short bud development time and flower longevity, and small seed sizes. Such associations evolved as a result of a strong r selection that maximizes population growth rate (Snell and Aarssen 2005) by producing many offspring with low surviving probability to adulthood. Most selfing species show hermaphrodite flowers and in some outcrossing occurs up to 5 %, which may be affected by humidity, temperature, and location. There are various mechanisms promoting selfing, for example, cleistogamy ensures complete selfing because foreign pollen cannot reach the stigma of a closed flower that does not open at all. Flowers open but after pollination in some small grain cereals such as rice or wheat, or the anthers surround closely the stigma in some crops of the nightshade family such as tomato. Selfing leads to homozygosity, but selfing species do not show inbreeding depression but may exhibit heterosis. Hence, selfing species cultivars may be inbred lines or hybrids. Composites and multilines are two other cultivar types. The former includes closely related lines such as isogenic (or isolines), while the latter may comprise inbred lines, hybrids, and populations sharing common traits. The methods for crossbreeding selfing species are mass selection, pedigree, bulk, single seed-descent, backcrossing, hybridization, and population improvement through recurrent selection. Plant genetic engineering, DNA marker-assisted breeding, and genomic selection may also be used for breeding selfing species.

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Haploidy in Cultivated Wheats: Induction and Utility in Basic and Applied Research

Cited by 60 publications

References 144 publications

Microspore embryogenesis and production of haploid and doubled haploid plants in carrot (Daucus carota L.)

Microspore embryogenesis and production of haploid and doubled haploid plants in carrot (Daucus carota L.)

Understanding grain yield: it is a journey, not a destination

Breeding Self-Fertilizing Plants: From Inbred to Hybrid Cultivars

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