Haplotype comparisons of Echinococcus granulosus sensu lato via mitochondrial gene sequences (co1, cytb, nadh1) among Pakistan and its neighbouring countries

Khan, Aisha; Ahmed, Haroon; Şimşek, Sami; Shahzad, Khuram; Çelik, Figen; Afzal, Muhammad Sohail; Khan, Mobushir Riaz; Liu, Hua; Shen, Yujuan; Cao, Jianping

doi:10.1017/s0031182021000688

Cited by 3 publications

(1 citation statement)

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“…Asexual reproduction of Echinococcus spp. occurs during the larval stage, which can result in numerous genetic mutations and variations at both the genus and species level [ 14 , 15 ].…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity and haplotype analysis of cattle hydatid cyst isolates using mitochondrial markers in Kazakhstan

Uakhit,

Yalysheva,

Abdybekova

et al. 2024

Vet World

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Background and Aim: In Kazakhstan, the study of Echinococcus infection among farm animals is crucial to monitor the invasion among livestock and map the data obtained. Unfortunately, there are only partial data on the study of Echinococcus among cattleâ€™s in Kazakhstan, which makes it difficult to conduct a comparative analysis of the epidemiological situation among livestock animals. The present study aimed to molecularly identify the species and haplotypes of the E. granulosus complex infecting cattle in Kazakhstan and investigate their genetic variation relative to mitochondrial (mt) targets. Materials and Methods: Individual cyst isolates (n = 700) were collected from infected cattle lungs and livers after slaughter from the slaughterhouse. Total DNA was extracted from the germinal layers of the cyst from each isolate. This DNA sequenced partial mt genes of cytochrome c oxidase 1 (450 bp) and NADH dehydrogenase 1 (1200 bp). Results: We determined that all the sequences were detected as E. granulosus s.s., of which 69 (94.5%) samples belonged to G1, and only 4 (5.4%) samples belonged to the G3 genotype. After bioinformatic analysis, 38 haplotypes were identified. Conclusion: Our findings revealed that the G1 genotype of E. granulosus s.s. is the predominant cattle genotype in Kazakhstan. However, only one region showed the presence of two genotypes G1 and G3, in the sequence, which suggests that further research is needed to investigate the epidemiology of Echinococcus infection in cattle in Kazakhstan. Keywords: cattle, cystic echinococcosis, Echinococcus granulosus, genotypes, haplotype, Kazakhstan.

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“…Asexual reproduction of Echinococcus spp. occurs during the larval stage, which can result in numerous genetic mutations and variations at both the genus and species level [ 14 , 15 ].…”

Section: Discussionmentioning

confidence: 99%