Haplotype frequencies of 17 Y-chromosomal short tandem repeat loci from the Cukurova region of Turkey

Abstract

AimTo investigate the distribution of 17 Y-short tandem repeat (STR) loci in the population of the Cukurova region of Turkey.MethodsIn the period between 2009 and 2010, we investigated the distribution of 17 Y-STRs in a sample of 249 unrelated healthy men from the Cukurova region of Turkey. Genomic DNA was extracted with InstaGene matrix and Y-STRs were determined using the AmpFISTR Yfiler PCR amplification kit. Gene and haplotype diversity values were estimated using the Arlequin software. To compare our data to other populations, population pairwise genetic distances and associated probability values were calculated using the Y Chromosome Haplotype Reference Database Web site software.ResultsAt 17 Y-STR loci we detected 148 alleles. The lowest gene diversity in this region was 0.51 for DYS391 and the highest 0.95 for DYS385a/b. Haplotype diversity was 0.9997 ± 0.0004. We compared our data with haplotype data of other Turkish populations and no significant differences were found, except with Ankara population (Φst = 0.025, P = 0.018). Comparisons were also made with the neighboring populations using analysis of molecular variance of the Y-STR loci genetic structure and our population was nearest to Lenkoran-Azerbaijani (Φst = 0.012, P = 0.068) and Iranian Ahvaz population (Φst = 0.007, P = 0.173), followed by Greek (Φst = 0.026, P = 0.000) and Russian (Φst = 0.048, P = 0.000) population. Other countries like Portugal, Spain, Italy, Egypt, Israel (Palestinian Authority Area), and Taiwan showed a high genetic distance from our population.ConclusionOur study showed that Y-STR polymorphisms were a powerful discrimination tool for routine forensic applications and could be used in genealogical investigations.

Genetic analysis of haplotype data for 23 Y-chromosome short tandem repeat loci in the Turkish population recently settled in Sarajevo, Bosnia and Herzegovina

“…Such results are probably a consequence of using PowerPlex Y 23 kit, which types results on 23 Y-STR loci, thus enabling increased haplotype diversity to be observed. A large number of identified alleles also confirm high haplotype diversity due to the use of 23 Y-STR loci, while previous studies of Turkish population showed a much smaller number of alleles due to the use of fewer Y-STR loci ( 1 , 21 - 24 ).…”

“…The most distant populations were those from the Balkan Peninsula: Bosnian, Croatian, and Macedonian. Another study has also found important differences between Turkish population and European populations ( 1 ). However, there are no studies comparing Turkish population with a greater number of other world populations, especially when it comes to the comparison over 23 Y-STRs.…”

“…Typing is performed using polymerase chain reaction (PCR), which is a reliable procedure tolerant to degraded DNA. Thus, Y-STRs can be used in forensics for the investigation of sexual assault cases, for deficient paternity testing when the alleged father is not available for testing, in gang rape situations (mixture of two or more male DNA samples), for the investigation of genetic reasons of male infertility, in genealogical research, particularly for surname testing, in population genetic studies, for the verification of amelogenin Y-deficient men, and in genetic epidemiology ( 1 - 9 ). In this study genotyping was performed by PowerPlex Y 23 kit (Promega Corporation, Madison, WI, USA).…”

A glimpse at the intricate mosaic of ethnicities from Mesopotamia: Paternal lineages of the Northern Iraqi Arabs, Kurds, Syriacs, Turkmens and Yazidis

“…Along with the five novel Y-STR datasets from the current study, the following population datasets with equivalent loci coverages were included during analysis: Cyprus [Greek Cypriot] ( n = 344) [ 19 ]; Cyprus [Greek Cypriot II] ( n = 574) [ 20 ]; Iran [East Iranian] ( n = 200) and Iran [West Iranian] ( n = 124) [ 21 ]; West Asia [Armenian, Erzurum origin] ( n = 99), West Asia [Armenian, Hemsheni] ( n = 89), West Asia [Armenian, Krasnodar] ( n = 117), West Asia [Armenian, Adygei] ( n = 49), West Asia [Armenian, Don] ( n = 92) [ 22 ]; Greece [Greek] ( n = 214), Iraq [Iraqi] ( n = 124), Barcelona, Spain [Spanish] ( n = 78), Bohemia, Czechia [Czech] ( n = 72), Hungary [Hungarian] ( n = 143), Upper Bavaria, Germany (German) ( n = 200), Bosnia and Herzegovina [Bosnian] ( n = 100), Marche, Italy [Italian] ( n = 170), Sicily, Italy [Italian] ( n = 157), Central Poland [Polish] ( n = 102), Central England [English] ( n = 81), Lebanon [Lebanese] ( n = 505), Beijing, China [Han] ( n = 246), Ibadan, Nigeria [Yoruba] ( n = 81), Kinyawa, Kenya [Maasai] ( n = 100), Philippines [Filipino] ( n = 169), Southern India, India [Tamil] ( n = 126) and Tokyo, Japan [Japanese] ( n = 59) [ 23 ]; Iraq [Iraqi II] ( n = 400) [ 24 ]; Lebanon [Maronite] ( n = 196) [ 21 ]; Cyprus [Turkish Cypriot] ( n = 380) [ 13 ]; Afghanistan [Turkmen] ( n = 73) [ 25 ]; Uzbekistan [Turkmen] ( n = 83) [ 26 ]; Marmara Region, Turkey [Turkish] ( n = 385) [YHRD Accession No. : YA004119]; Cukurova, Turkey [Turk] ( n = 249) [ 27 ]; and Southeastern Anatolia, Turkey [Turkish] ( n = 86+64) [ 28 ] and [YHRD Accession No. : YA004118].…”

Comparison of the Frequency of Y-short Tandem Repeats Markers between Sadat and Non-Sadat Populations in Isfahan Province of Iran

“…The results also showed that Y-STR polymorphisms are a powerful discrimination tool for routine forensic applications and can be used in anthropological investigations. [ 16 ]…”