Hapten-reactive inducer T cells. I. Definition of two classes of hapten-specific inducer cells.

Clayberger, Carol; Aisenberg, James; Cantor, Harvey

doi:10.1084/jem.157.6.1906

Cited by 46 publications

(20 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nevertheless, studies employing anti-hapten antibodies proved that the proliferative response of primed T cells or trinitrophenoi (TNP)-specific T cell clones could be considerably impaired by the use of anti-TNP antibodies [9,38]. Similarly, inhibition ofT cell proliferation by antigenspecific antibody was demonstrated using lymphocytes specific for apo-cytochrome C [11].…”

Section: Discussionmentioning

confidence: 98%

Cell-mediated immunity to chemically xenogenized tumors I. Inhibition by specific antisera and H-2 association of the novel antigens

Romani

Grohmann

Fazioli

et al. 1988

Cancer Immunol Immunother

View full text Add to dashboard Cite

T cell-mediated proliferative and cytotoxic responses occur in vitro to syngeneic tumor cells antigenically altered by mutagen treatment. One such xenogenized variant of the murine L5178Y lymphoma elicits IgG antibodies reactive with determinants on variant cells that are not expressed at detectable levels on parental or normal cells of the same H-2d haplotype and are also unrelated to public specificities of H-2b or H-2k histocompatibility antigens. In the present study we investigated the effect of those antibodies on development of cell-mediated responses in vitro to the xenogenized cells used for induction of the humoral response. The proliferative reaction, generation of cytolytic activity and target cell lysis were all inhibited by the anti-xenogenized tumor immune serum, whereas the corresponding reactions to the parental cells by syngeneic or allogeneic effector lymphocytes were not. In order to investigate the possible H-2 association of T cell-mediated responses to xenogenized cells, we also examined the effect on those reactions of antibodies specific for Class I or Class II products of the H-2d complex. The results obtained suggested a role for I-Ad molecules in the T cell proliferative response to the xenogenized cells, and also indicated a preferential association of the cytotoxic response with H-2Kd determinants.

show abstract

Section: Discussionmentioning

confidence: 98%

Cell-mediated immunity to chemically xenogenized tumors I. Inhibition by specific antisera and H-2 association of the novel antigens

Romani

Grohmann

Fazioli

et al. 1988

Cancer Immunol Immunother

View full text Add to dashboard Cite

show abstract

“…Fig. 9A demonstrates that HKL induced a significant quantity of IL-18 mRNA expression after 8, 16, and 24 h of culture. Moreover, treatment of mice in vivo with OVA with HKL, but not with OVA alone, induced IL-18 mRNA expression in draining LN (Fig.…”

Section: Hkl As An Adjuvant Increases Il-18-mrna Expression In Spleenmentioning

confidence: 99%

“…LN cells (5 ϫ 10 5 cells/well) were restimulated in vitro with OVA in DMEM (Life Technologies, Grand Island, NY), which was supplemented as previously described (16), and contained 5 ϫ 10 Ϫ5 M 2-ME and 10% FCS (HyClone Laboratories, Logan, UT). Cells were cultured in 96-well microtiter plates in 150 l medium.…”

Section: Restimulation Of Ln Cells In Vitromentioning

confidence: 99%

Vaccination with Heat-KilledListeriaas Adjuvant Reverses Established Allergen-Induced Airway Hyperreactivity and Inflammation: Role of CD8+ T Cells and IL-18

Hansen¹,

Yeung²,

Berry

et al. 2000

The Journal of Immunology

105

View full text Add to dashboard Cite

Asthma is a respiratory disorder characterized by airway hyperreactivity (AHR) and inflammation and is associated with high serum IgE and overproduction of IL-4, IL-5, and IL-13 by allergen-specific Th2 cells. Our previous studies demonstrated that heat-killed Listeria monocytogenes (HKL) as an adjuvant in immunotherapy successfully reversed ongoing Ag-specific Th2-dominated responses toward Th1-dominated responses, but it was unclear if such immune modulation could reverse ongoing, established disease in target organs such as the lung. In this paper we show that a single dose of Ag plus HKL as adjuvant significantly reduced AHR in a murine model for asthma and reversed established AHR when given late after allergen sensitization. HKL as adjuvant also dramatically inhibited airway inflammation, eosinophilia, and mucus production, significantly reduced Ag-specific IgE and IL-4 production, and dramatically increased Ag-specific IFN-γ synthesis. The inhibitory effect of HKL on AHR depended on the presence of IL-12 and CD8+ T cells and was associated with an increase of IL-18 mRNA expression. Thus, our results demonstrate that HKL as an adjuvant for immunotherapy mediates immune deviation from a pathological Th2-dominated response toward a protective immune response in peripheral lymphoid tissues and in the lungs and may be clinically effective in the treatment of patients with established asthma and allergic disease.

show abstract

“…To test this hypothesis, additional cDNA clones were isolated either from transformed Meth A fibroblasts, from which p53-M8 was previously isolated (34), or from a normal T-cell library (6), and the structures of their encoded proteins were compared. The following cDNAs were studied in detail: p53-Mll isolated from the Meth A cDNA library of BALB/c origin and the pCD-p53 cDNA clone isolated from a nontransformed helper T-cell clone, E.1 (6), which was reactive to hapten trinitrophenol on a BALB/c background.…”

Section: Resultsmentioning

confidence: 99%

Immunologically distinct p53 molecules generated by alternative splicing.

Arai¹,

Nomura²,

Yokota³

et al. 1986

Mol. Cell. Biol.

104

View full text Add to dashboard Cite

Transfection of a functional cloned p53 gene into an L12 p53 nonproducer cell line efficiently reconstituted p53 expression. The p53 protein synthesized in these clones was indistinguishable from that occurring naturally in tumor cells. When a p53 cDNA clone was used instead, we observed that the L12-derived clones exhibited a distinct immunological profile. In the present experiments we compared the immunological epitopes of p53 proteins encoded by several full-length cDNA clones. Immunoprecipitation of p53 proteins generated by in vitro transcription and translation of the various cDNA clones indicated variations in the content of immunological epitopes. Basically, two p53 protein species were detected. Both species contained the same antigenic determinants except the PAb421-PAb122 site, which was present in proteins encoded by p53-Mll and pcD-p53, but not in the p53 protein encoded by the p53-M8 cDNA clone. Sequence analysis of the various cDNA clones indicated the existence of a 96-base-pair (bp) insert in clone p53-M8 as compared with clone p53-Mll or pCD-p53. The 96-bp insert contained a termination signal which caused the premature termination of the protein, leading to the generation of a p53 product 9 amino acids shorter than usual. The existence of this insert also accounted for the lack of the PAb421-PAb122 epitope which was mapped to the 3' end of the cDNA clone, following the 96-bp insert. This insert shared complete homology with the p53 intron 10 sequences mapping 96 bp upstream of the 5' acceptor splicing site of p53 exon 11. It was therefore concluded that the different cDNA clones represented p53 mRNA species which were generated by an alternative splicing mechanism. Differential hybridization of the mRNA population of transformed fibroblastic or lymphoid cells with either the 96-bp synthetic oligonucleotide or the p53-M11 cDNA indicated that the various mRNA species are expressed in vivo.

show abstract

Hapten-reactive inducer T cells. I. Definition of two classes of hapten-specific inducer cells.

Cited by 46 publications

References 40 publications

Cell-mediated immunity to chemically xenogenized tumors I. Inhibition by specific antisera and H-2 association of the novel antigens

Cell-mediated immunity to chemically xenogenized tumors I. Inhibition by specific antisera and H-2 association of the novel antigens

Vaccination with Heat-KilledListeriaas Adjuvant Reverses Established Allergen-Induced Airway Hyperreactivity and Inflammation: Role of CD8+ T Cells and IL-18

Immunologically distinct p53 molecules generated by alternative splicing.

Contact Info

Product

Resources

About