Hapten synthesis and development of ELISAs for detection of endosulfan in water and soil.

Lee, Nanju Alice; Skerritt, John H.; McAdam, David P.

doi:10.1021/jf00054a060

Cited by 69 publications

(77 citation statements)

References 20 publications

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“…Although the usual concentration of Tween 20 for most pesticide immunoassays is 0.05%, for several pesticides a marked effect of detergent concentration on ELISA performance has been observed. Affinities can be increased by reducing the concentration of surfactant in the competition buffer, or even eliminating it completely [24]. In this study (Fig.…”

Section: Elisa Optimizationmentioning

confidence: 83%

Assessment of enzyme-linked immunosorbent assay for the determination of 2,4,5-TP in water and soil

et al. 2002

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A highly sensitive and specific indirect enzyme-linked immunosorbent assay is described for Silvex, 2-(2,4,5 trichlorophenoxy)propionic acid, (2,4,5-TP). One specific feature of the immunoassay is the use of simple chemical activation of chlorophenoxy acids to prepare both the immunizing and coating conjugates. The assay is based on the use of polyclonal antibodies raised against 2,4,5-TP, and a peroxidase-labeled secondary antibody for colorimetric detection. The effect of different chemical conditions (pH, and salt and detergent concentration) on immunoassay performance has been studied. Under the best conditions the least detectable dose and the sensitivity (IC(50)) for 2,4,5-TP were 0.05 micro g L(-1) and 0.80 micro g L(-1), respectively. The optimized immunoassay was also highly specific, showing little (6.9% for 2,4,5-T) or no cross-reactivity with other similar herbicides. The assay was used to determine 2,4,5-TP in water and soils. The excellent recoveries obtained (mean values ranging between 89% and 104%) make this immunoassay a suitable screening method for either environmental monitoring or laboratory quantification of 2,4,5-TP.

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Section: Elisa Optimizationmentioning

confidence: 83%

Assessment of enzyme-linked immunosorbent assay for the determination of 2,4,5-TP in water and soil

et al. 2002

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“…As shown in Table 2, the highest sensitivity was achieved using glycine buffer at pH 10.0, which was selected for routine use. Other factors such as addition of bovine serum albumin to the PBST buffer (Lee et al, 1995) or buffer ionic strength (Lee et al, 1995) (data not shown) did not improve the sensitivity of the assay. Finally, the optimized ELISA, whose features are specified in the four parameter equation in Figure 1, allows the measurement of PBAN in a concentration range between 30 to 490 pM with an I 50 = 81 ± 9 pM.…”

Section: Immunoassay Developmentmentioning

confidence: 90%

Temporal distribution of PBAN-like immunoreactivity in the hemolymph ofMamestra brassicae females in relation to sex pheromone production and calling behavior

Iglesias

Jacquin‐Joly

Marco

et al. 1999

Arch. Insect Biochem. Physiol.

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A direct enzyme‐linked immunosorbent assay has been developed and applied to the analysis of PBAN immunoreactivity in female hemolymph of the cabbage armyworm, Mamestra brassicae. PBAN‐IR determinations have been carried out with third scotophase insects at different times of the photoperiod. The rhythm of calling and the pattern of pheromone production by third scotophase females at different times of the photoperiod have also been determined. PBAN‐IR and calling are well correlated. However, whereas pheromone titers decrease, both PBAN‐IR levels and percentage of calling females remain high in the last hours of the scotophase. These results are discussed in the context of the regulation of sex pheromone biosynthesis in M. brassicae. Arch. Insect Biochem. Physiol. 40:80–87, 1999. © 1999 Wiley‐Liss, Inc.

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“…The coupling reaction to KLH was carried out using a method previously described [18]. N-hydroxysuccinimide (0.047 mmol) and N,N'-diisopropylcarbodiimide (9.3 μmol), dissolved in 600 μl of dimethylformamide (DMF), were added to AnAFB 1 (5.19 μmol) in 600 μl of dry dichloromethane at 0°C, followed by 4-(dimethylamino)pyridine (0.041 mmol).…”

Section: Methodsmentioning

confidence: 99%

Vaccination of Heifers with Anaflatoxin Improves the Reduction of Aflatoxin B1 Carry Over in Milk of Lactating Dairy Cows

et al. 2014

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It was previously reported that injection of anaflatoxin B1 (AnAFB1) conjugated to keyhole limpet hemocyanin (KLH), together with Freund's adjuvant, was effective in inducing in cows a long lasting titer of anti-aflatoxin B1 (AFB1) antibodies (Abs), cross-reacting with other aflatoxins, which were able to hinder, proportionally to their titer, the secretion of aflatoxin M1 (AFM1) into the milk of cows continuously fed with AFB1. According to anti-AFB1 Ab titer, 50% of the vaccinated cows were recognized as high responder animals. In an attempt to prepare a more effective formulation for vaccination of cows, it was compared the immunogenicity, in Holstein Friesian heifers, of AnAFB1 covalently conjugated to KLH or to recombinant diphtheria toxin (CRM197) molecules, and injected together with various adjuvants. This study demonstrated that injection of AnAFB1 conjugated to KLH and mixed with complete (priming) and incomplete Freund's adjuvant (boosters), as in the previous schedule of immunization, was the most effective regimen for inducing Ab responses against AFB1, although pre-calving administration could increase the effectiveness of vaccination, resulting in 100% high responder animals. After one booster dose at the beginning of the milk production cycle, anti-AFB1 Ab titers were comparable to those recorded at the end of the immunization schedule, and proved to be effective in reducing significantly AFB1 carry over, as AFM1, from feed to milk. Pre-calving vaccination of dairy heifers with conjugated AnAFB1, adjuvated with complete and incomplete Freund's adjuvant, may represent the most effective tool for preventing the public health hazard constituted by milk and cheese contaminated with aflatoxins.

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Hapten synthesis and development of ELISAs for detection of endosulfan in water and soil.

Cited by 69 publications

References 20 publications

Assessment of enzyme-linked immunosorbent assay for the determination of 2,4,5-TP in water and soil

Assessment of enzyme-linked immunosorbent assay for the determination of 2,4,5-TP in water and soil

Temporal distribution of PBAN-like immunoreactivity in the hemolymph ofMamestra brassicae females in relation to sex pheromone production and calling behavior

Vaccination of Heifers with Anaflatoxin Improves the Reduction of Aflatoxin B1 Carry Over in Milk of Lactating Dairy Cows

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