Hapten synthesis and production of specific antibody against 3-amino-5-morpholinomethyl-2-oxazolidone for immunoassay without derivatisation

Abstract

2018) Hapten synthesis and production of specific antibody against 3-amino-5-morpholinomethyl-2-oxazolidone for immunoassay without derivatisation, Food and Agricultural Immunology, 29:1, 332-345, ABSTRACT Immunoassay direct for 3-amino-5-morpholinomethyl-2-oxazolidone (AMOZ), the metabolite of furaltadone, based on a specific antibody was usually unavailable due to its small molecule weight. In this work, a hapten with a double bond and an active carboxyl group was designed and then conjugated to peptide dendrimer to obtain novel multiple haptens. The haptens and multiple haptens were coupled to bovine serum albumin as immunogens. Polyclonal antibodies showed binding to free AMOZ in a heterologous competitive indirect enzyme-linked immunosorbent assay were obtained. The assay showed IC 50 (half maximal inhibitory concentration) of 4.1 μg/kg and limit of detection of 0.2 μg/kg for AMOZ. Recoveries of AMOZ from grass carp were tested from 83.1% to 117.0%, with the coefficient of variation below 12%. As no laborious derivatisation procedure was employed, the proposed assay should be useful for the screening of AMOZ for a large number of samples. ARTICLE HISTORY

Design of Novel Haptens and Development of Monoclonal Antibody-Based Immunoassays for the Simultaneous Detection of Tylosin and Tilmicosin in Milk and Water Samples

“…It was reported that more similar the characteristics of a hapten derivative are to the target, such as size, shape (geometry) and electronic properties, the more likely specific antibodies will be produced [38,39]. Moreover, it is also suggested that a short semi-rigid unsaturated double bond structure is generally good for the production of desired antibodies [40][41][42]. Based on these ideas, haptens with short spacer arms or unsaturated double bond arms were designed and the similarity between drugs and haptens was analyzed by molecular simulation.…”

“…In summary, it was an efficient strategy for target-derived hapten to be designed rationally through structure simulation, and further confirmed that the well-mimicked hapten TYL-CHO had stable immunogenicity to induce an antibody against tylosin in mice (Table 1). suggested that a short semi-rigid unsaturated double bond structure is generally good for the production of desired antibodies [40][41][42]. Based on these ideas, haptens with short spacer arms or unsaturated double bond arms were designed and the similarity between drugs and haptens was analyzed by molecular simulation.…”

Improved molecular softness of tadalafil hapten enhancing antibody performance in immunoassay: Evidence from computational chemistry

“…However, how could haptens be designed to produce antibodies with an excellent and desired analytical performance such as broad group specificity, high sensitivity, and good affinity? Our past and ongoing research about hapten design for achieving a desired antibody had also yielded some useful results and discoveries such as unsaturated arm strategy (Huang et al, 2019;Liu et al, 2017;Luo et al, 2019;Shen et al, 2007;Yang et al, 2013), molecular orbital energy theory (Xu et al, 2010), electrostatic potential (ESP) and lipophilicity (LP) factors (He et al, 2021;Huang et al, 2019), etc. All the above strategies could increase hapten-specific immune efficacy to some extent.…”

Development of a single-chain variable fragment-alkaline phosphatase fusion protein-based direct competitive enzyme-linked immunosorbent assay for furaltadone metabolite in ctenopharyngodon idellus

“…Furaltadone (Figure S1), a kind of nitrofuran antibiotic, was once widely used in the livestock, poultry and aquaculture industries due to its dominant antibacterial activity. Howerve, it has been banned for using in edible animals in many countries attributing to furaltadone and its metabolites are all highly toxic, teratogenic, carcinogenic and mutagenic (Guo et al, 2017;Li et al, 2017;Liu et al, 2018). It is disappointed that application of furaltadone illegal is still very serious in many countries, including China, because of its good efficacy and low price.…”

“…Compared with instrumental methods such as high-performance liquid chromatography (HPLC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS), immunoassays possess the advantages of being simple, fast, sensitive and low cost (Van Emon, 2010;Wang et al, 2016a). As a high-throughput screening method, immunoassays have already been explored and employed in the field of rapid determination of AMOZ residue in edible animal tissues (Dai et al, 2015;Deng et al, 2016;Jester et al, 2014;Li et al, 2014;Liu et al, 2018;Shen et al, 2012;Xie et al 2019;Xu et al, 2013aXu et al, , 2013b.…”