Hapten synthesis, monoclonal antibody production and development of a competitive indirect enzyme-linked immunosorbent assay for erythromycin in milk

A novel immunoassay for quantitative drug abuse screening in serum

“…After an elaborate antibody validation benzoylecgonine, MDMA and methadone were detectable and quantifiable specifically and sensitively. Comparing the results to other competitive immunoassays for substances like antibiotics (Huang et al, 2010;Jiang et al, 2013;Wang et al, 2015), similar detection limits and CVs were obtained. In relation to one of the standard application of immunoassays for drug abuse (lateral flow assays) better detection limits and quantification could be reached (Attema-de Jonge et al, 2012;Taylor and Pizzo, 2004).…”

Sensitive immunoassay for simultaneous determination of tetrabromobisphenol A bis(2-hydroxyethyl) ether and tetrabromobisphenol A mono(hydroxyethyl) ether: An effective and reliable strategy to estimate the typical tetrabromobisphenol A derivative and byproduct in aquatic environments

“…When comparing the observed molecular weights of BSA-M3 with the BSA (Fig. S4), the value fell within the range of 8:1 to 25:1, which was the best range for antibody production (Wang et al, 2015). So our synthesized immunogen would lead to suitable antibodies as anticipated in our original strategy.…”

“…In addition, the coating antigen was obtained using similar methods and only the conjugated protein was replaced with OVA. The value of hapten to protein conjugates was identified with MOLDI-TOF/MS by comparing the observed molecular weights of the prepared conjugates with the unreacted protein (Wang et al, 2015).…”

“…However, the antisera from the rabbit R3-2 showed the best affinity to TBBPA DHEE (IC 50 value, 31 ng/mL) when the antisera were diluted to 12,000 times. The above-noted observations indicated that there was no certain relationship between antibody sensitivity and titer (Wang et al, 2015). Due to better sensitivity, the antisera from the rabbit R3-2 was used in the subsequent study.…”

Immunochemical detection of emerging organic contaminants in environmental waters

“…In contrast to the approaches above, another approach to generate an antibody capable of simultaneously recognizing compounds with diverse molecular structures is to conjugate several haptens to the same carrier protein [43]. However, the challenge of this approach is how to control the number of each hapten derivative binding to the correspondent carrier protein, even the value of hapten to protein conjugates could be identified by MOlDI-TOF/MS through comparing the observed molecular weights of the prepared conjugates with the unreacted protein [44].…”