Haptoglobin and fibrinogen concentrations and leukocyte counts in the clinical investigation of caseous lymphadenitis in sheep

Bastos, Bruno Lopes; Meyer, Roberto; Guimarães, José Eugênio; Ayres, Maria Consuêlo Caribé; Guedes, Maria Tereza B.; Moura-Costa, Lília Ferreira de; Burghgrave, Uiara S. de; Sena, Ludmilla Soares; Azevedo, Vasco; Portela, Ricardo Wagner

doi:10.1111/j.1939-165x.2011.00355.x

Cited by 21 publications

(20 citation statements)

References 23 publications

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“…In some specific infectious conditions such as caseous lymphadenitis in sheep, APPs can differentiate between acute (with increases in SAA and Hp) and chronic states (with increases in AGP) [244]. It has been also suggested as a potential marker for progression of this disease because sheep that did not develop peripheral abscesses had a higher haptoglobin concentration during the acute phase of the disease compared with the sheep that developed abscesses [247].…”

mentioning

confidence: 97%

Acute phase proteins in ruminants

Ceciliani

Cerón

Eckersall

et al. 2012

Journal of Proteomics

460

432

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mentioning

confidence: 97%

Acute phase proteins in ruminants

Ceciliani

Cerón

Eckersall

et al. 2012

Journal of Proteomics

460

432

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“…Hence, the quantification of APP can be a way to determine the health status of an animal or a herd [57] and thus be used to assess disease pathogenesis, the evolution of an infection or the efficacy of drugs and vaccines. For example, CLA-affected sheep have been described to display higher serum concentrations of Hp, SAA and AGP [55,58]. Although the validation of analytical methods and reference values of SAA and Hp in Iberian ibex have been provided recently [33], we were not able to detect significant changes in Hp, SAA nor ASG.…”

Section: Discussionmentioning

confidence: 54%

“…Monocyte cell counts were also higher with regard to values previously published [49], which could be associated with stress induced by the box-trap capture method or to subclinical inflammatory conditions [54]. Although the leukogram is considered a useful tool for monitoring infectious processes, ruminants may show no or few changes even during severe infections [55]. Haemoglobin concentration and PCV increased from t2 to t3.…”

Section: Discussionmentioning

confidence: 85%

Management of a caseous lymphadenitis outbreak in a new Iberian ibex (Capra pyrenaica) stock reservoir

et al. 2014

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BackgroundIn 2010, an Iberian ibex (Capra pyrenaica hispanica) stock reservoir was established for conservation purposes in north-eastern Spain. Eighteen ibexes were captured in the wild and housed in a 17 hectare enclosure. Once in captivity, a caseous lymphadenitis (CLA) outbreak occurred and ibex handlings were carried out at six-month intervals between 2010 and 2013 to perform health examinations and sampling. Treatment with a bacterin-based autovaccine and penicillin G benzatine was added during the third and subsequent handlings, when infection by Corynebacterium pseudotuberculosis was confirmed. Changes in lesion score, serum anti-C. pseudotuberculosis antibodies and haematological parameters were analyzed to assess captivity effects, disease emergence and treatment efficacy. Serum acute phase proteins (APP) Haptoglobin (Hp), Amyloid A (SAA) and Acid Soluble Glycoprotein (ASG) concentrations were also determined to evaluate their usefulness as indicators of clinical status.Once in captivity, 12 out of 14 ibexes (85.7%) seroconverted, preceding the emergence of clinical signs; moreover, TP, WBC, eosinophil and platelet cell counts increased while monocyte and basophil cell counts decreased. After treatment, casualties and fistulas disappeared and both packed cell volume (PCV) and haemoglobin concentration significantly increased. Hp, SAA and ASG values were under the limit of detection or showed no significant differences.ConclusionsA role for captivity in contagion rate is suggested by the increase in antibody levels against C. pseudotuberculosis and the emergence of clinical signs. Although boosted by captivity, this is the first report of an outbreak of caseous lymphadenitis displaying high morbidity and mortality in wild ungulates. Treatment consisting of both vaccination and antibiotic therapy seemed to prevent mortality and alleviate disease severity, but was not reflected in the humoural response. Haematology and APP were not useful indicators in our study, perhaps due to the sampling frequency. Presumably endemic and irrelevant in the wild, this common disease of domestic small ruminants is complicating conservation efforts for the Iberian ibex in north-eastern Spain.

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“…Nowroozi-Asl et al (2008) publicaram valores de 5 a 18mg/dL como referência para animais sadios, utilizando a raça Rabo Largo Iranianos. Outros trabalhos envolvendo a haptoglobina como marcador inϐlamatório em ovinos foram publicados (Regassa & Noakes 1999, Ulutas & Ozpinar 2006, Eckersall et al 2007, González et al 2007, Lepherd et al 2009), alguns inclusive utilizando ovinos criados no Brasil que poderiam servir como parâmetro (Pereira et al 2009, Costa et al 2010, Bastos et al 2011, Chaves et al 2011, apesar de nenhum estabelecer valores de referên-cia para as raças utilizadas. Em estudo recente, Kostro et al (2009) estudaram ovinos antes e após transporte e abate para comparar valores de HP com estresse e lesões observadas na necropsia.…”

Section: Discussionunclassified

“…Isto está de acordo com os resultados de Borges et al (2006) onde não foi observado aumento signiϐicativo do ϐibrinogênio em bovinos com pododermatite. Embora o ϐibrinogênio seja uma proteína bastante conhecida e mundialmente utilizada principalmente na clínica médica da espécie bovina, na espécie ovina ele não se mostrou um marcador sensível para a pododermatite, conforme o delineamento experimental, bem como para outras enfermidades de ovinos (Bastos et al 2011). Isto pode estar relacionado a características de sua cinética na espécie objeto de estudo bem como também a localização do processo inϐlamatório circunscrito ao tecido podal.…”

Section: Discussionunclassified

Evolução clínica e avaliação de parâmetros leucocitários e de proteínas de fase aguda na pododermatite infecciosa ovina

Carvalho

Araújo²,

Vasconcelos³

et al. 2012

Pesq. Vet. Bras.

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ABSTRACT.-Carvalho V.S., Araújo B.R., Vasconcelos T.C., Chiminazzo C., Costa Neto A.O., Ayres M.C.C., Guimarães J. In this paper, Santa Inês crossbred sheep with footrot were evaluated, checking the changes on leukogram and the acute phase proteins. In the ϐirst experiment, 70 females were divided into three groups according to their podal scores: a control group (G1) with score 0, scald group (G2) with score 1 or 2 and a footrot group (G3) with score 3, 4 or 5. During two months, the clinical course and its effect on leukogram were observed at ϐive moments: M1 (day 0), M2 (day 15), M3 (day 30), M4 (day 45) and M5 (day 60). The intensity of the white blood cell count was low magnitude, observing signiϐicant changes (p <0.05) as a mild leukocytosis (G3, M4), attributed to neutrophilia and a slight increase in the total number of monocytes (G3, M2 and M3) despite the extensive damage and tissue necrosis existed during the ϐinal stage of the disease. In the second experiment, 105 animals from seven farms were used with the objective of isolating the causative agent and to evaluate the effect of disease on total plasma protein and acute phase proteins. The animals were also divided into three groups: control, scald and footrot. In all farms, the isolation of Dichelobacter nodosus was regarded. For the different stages of the disease on the proteins studied there was no signiϐicant correlation (p<0.05) but the haptoglobin in scald and footrot groups showed higher average than the control group. Based on these results, we conclude that the acute phase proteins studied were not efϐicient in the characterization of the phases of the disease and the leukocyte response was mild, making it difϐicult to use for this purpose.

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Haptoglobin and fibrinogen concentrations and leukocyte counts in the clinical investigation of caseous lymphadenitis in sheep

Abstract: Serum haptoglobin concentration and monocyte counts may be potential markers for progression of CLA in sheep.

Cited by 21 publications

References 23 publications

Acute phase proteins in ruminants

Acute phase proteins in ruminants

Management of a caseous lymphadenitis outbreak in a new Iberian ibex (Capra pyrenaica) stock reservoir

Evolução clínica e avaliação de parâmetros leucocitários e de proteínas de fase aguda na pododermatite infecciosa ovina

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