Haptoglobina en líquido cefalorraquídeo como marcador de procesos infecciosos en el sistema nervioso central

Noris-García, Elena; Aj, Dorta-Contreras; Escobar-Pérez, X; González-Hernández, Marlen

doi:10.33588/rn.2902.99275

Cited by 2 publications

(1 citation statement)

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“…These proteins are synthesized from three common alleles, Hp lF , Hp 18 , and Hp 2 , whose combination leads to three major Hp phenotypes – Hp (1–1), Hp (2–1), and Hp (2–2). Hp is an acute‐phase protein, acts as an antioxidant, has antibacterial activity, and has also been proposed as a biomarker for the detection of various diseases . In addition, possession of a particular Hp phenotype has been associated with a variety of common disorders such as cardiovascular disease, autoimmune disorders, or malignancy .…”

Section: Introductionmentioning

confidence: 99%

Disulfide proteomics for identification of extracellular or secreted proteins

et al. 2012

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The combination of SDS-PAGE and MS is one of the most powerful and perhaps most frequently used gel-based proteomics approaches in protein identification. However, one drawback of this method is that separation takes place under denaturing and reducing (R) conditions and as a consequence, all proteins with identical apparent molecular mass (Mr) will run together. Therefore, low-abundant proteins may not be easily identified. Another way of investigating proteins by proteomics is by analyzing subproteomes from a total proteome such as phosphoproteomics, glycoproteomics, or disulfide proteomics. Here, we took advantage of the property of secreted proteins to form disulfide bridges and investigated disulfide-linked proteins, using SDS-PAGE under nonreducing (NR) conditions. We separated sera from normal subjects and from patients with various diseases by SDS-PAGE (NR) and (R) conditions, followed by LC-MS/MS analysis. Although we did not see any detectable difference between the sera separated by SDS-PAGE(R), we could easily identify the disulfide-linked proteins separated by SDS-PAGE (NR). LC-MS/MS analysis of the disulfide-linked proteins correctly identified haptoglobin (Hp), a disulfide-linked protein usually found as a heterotetramer or as a disulfide-linked heteropolymer. Western blotting under NR and R conditions using anti-Hp antibodies confirmed the LC-MS/MS experiments and further confirmed that upon reduction, the disulfide-linked Hp heterotetramers and polymers were no longer disulfide-linked polymers. These data suggest that simply by separating samples on SDS-PAGEunder NR conditions, a different, new proteomics subset can be revealed and then identified.

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Section: Introductionmentioning

confidence: 99%