Haralick's texture analysis to predict cellular proliferation on randomly oriented electrospun nanomaterials

Bloise, Nora; Fassina, Lorenzo; Focarete, Maria Letizia; Lotti, Nadia; Visai, Livia

doi:10.1039/d1na00890k

Cited by 5 publications

(3 citation statements)

References 38 publications

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“…The texture of a gray-level image can be calculated through Haralick features; therefore, it is possible to correlate these data with the observed biological parameters, namely the number of bacteria in planktonic culture. For each SEM image of the materials without bacteria, we have selected at least two regions of interest (ROIs) to measure the graylevel co-occurrence matrix (GLCM) [39]. Then, for each GLCM, we have calculated one Haralick's feature: the "correlation" [20].…”

Section: Texture Analysis Of Sem Imagesmentioning

confidence: 99%

Surface Properties of a Biocompatible Thermoplastic Polyurethane and Its Anti-Adhesive Effect against E. coli and S. aureus

Restivo,

Peluso,

Bloise

et al. 2024

JFB

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Thermoplastic polyurethane (TPU) is a polymer used in a variety of fields, including medical applications. Here, we aimed to verify if the brush and bar coater deposition techniques did not alter TPU properties. The topography of the TPU-modified surfaces was studied via AFM demonstrating no significant differences between brush and bar coater-modified surfaces, compared to the un-modified TPU (TPU Film). The effect of the surfaces on planktonic bacteria, evaluated by MTT assay, demonstrated their anti-adhesive effect on E. coli, while the bar coater significantly reduced staphylococcal planktonic adhesion and both bacterial biofilms compared to other samples. Interestingly, Pearson’s R coefficient analysis showed that Ra roughness and Haralick’s correlation feature were trend predictors for planktonic bacterial cells adhesion. The surface adhesion property was evaluated against NIH-3T3 murine fibroblasts by MTT and against human fibrinogen and human platelet-rich plasma by ELISA and LDH assay, respectively. An indirect cytotoxicity experiment against NIH-3T3 confirmed the biocompatibility of the TPUs. Overall, the results indicated that the deposition techniques did not alter the antibacterial and anti-adhesive surface properties of modified TPU compared to un-modified TPU, nor its bio- and hemocompatibility, confirming the suitability of TPU brush and bar coater films in the biomedical and pharmaceutical fields.

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Section: Texture Analysis Of Sem Imagesmentioning

confidence: 99%

Surface Properties of a Biocompatible Thermoplastic Polyurethane and Its Anti-Adhesive Effect against E. coli and S. aureus

Restivo,

Peluso,

Bloise

et al. 2024

JFB

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“…Surface texture of scaffolds was studied through two Haralick's surface features (energy and variance) [25], calculated starting from the SEM images obtained via secondary electrons (SEs) at 350× of magnification. This approach evaluates the surface texture using a gray-level image of the surface [26,27]. Briefly, for each SEM image of the scaffold surface, at least two regions of interest (ROIs) were selected to measure the gray-level co-occurrence matrix (GLCM) and then, for each GLCM, Haralick's energy and variance values were quantified.…”

Section: Haralick's Surface Analysismentioning

confidence: 99%

Combined Effects of HA Concentration and Unit Cell Geometry on the Biomechanical Behavior of PCL/HA Scaffold for Tissue Engineering Applications Produced by LPBF

Gatto,

Furlani,

Giuliani

et al. 2023

Materials

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This experimental study aims at filling the gap in the literature concerning the combined effects of hydroxyapatite (HA) concentration and elementary unit cell geometry on the biomechanical performances of additively manufactured polycaprolactone/hydroxyapatite (PCL/HA) scaffolds for tissue engineering applications. Scaffolds produced by laser powder bed fusion (LPBF) with diamond (DO) and rhombic dodecahedron (RD) elementary unit cells and HA concentrations of 5, 30 and 50 wt.% were subjected to structural, mechanical and biological characterization to investigate the biomechanical and degradative behavior from the perspective of bone tissue regeneration. Haralick’s features describing surface pattern, correlation between micro- and macro-structural properties and human mesenchymal stem cell (hMSC) viability and proliferation have been considered. Experimental results showed that HA has negative influence on scaffold compaction under compression, while on the contrary it has a positive effect on hMSC adhesion. The unit cell geometry influences the mechanical response in the plastic regime and also has an effect on the cell proliferation. Finally, both HA concentration and elementary unit cell geometry affect the scaffold elastic deformation behavior as well as the amount of micro-porosity which, in turn, influences the scaffold degradation rate.

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“…The wide range of methods used to investigate surface characteristics, such atomic force microscopy and scanning electron microscopy, can provide important information to enhance our understanding of cell behaviour on biomaterials. In this context, we recently reported that nanotopography-induced changes in cell phenotype and proliferation can be predicted via a computer vision approach [58], the so-called analysis of Haralick's features, where an imaging technique (e.g., SEM) is followed by a texture analysis of the obtained images [59]. Haralick's features are derived from the grey-level co-occurrence matrix (GLCM) and are utilised in many fields such as land-use and forest-type classification [60], fabric defect recognition [61] and in medicine: e.g., skin texture [62], MRI images of the liver [63], X-ray mammography [64], breast cancer [65], brain cancer [66], tumour phenotype [67], and tumour classification [68].…”

Section: Texture Analysis Of Sem Imagesmentioning

confidence: 99%

Early Osteogenic Marker Expression in hMSCs Cultured onto Acid Etching-Derived Micro- and Nanotopography 3D-Printed Titanium Surfaces

Bloise

Waldorff

Montagna

et al. 2022

IJMS

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Polyetheretherketone (PEEK) titanium composite (PTC) is a novel interbody fusion device that combines a PEEK core with titanium alloy (Ti6Al4V) endplates. The present study aimed to investigate the in vitro biological reactivity of human bone-marrow-derived mesenchymal stem cells (hBM-MSCs) to micro- and nanotopographies produced by an acid-etching process on the surface of 3D-printed PTC endplates. Optical profilometer and scanning electron microscopy were used to assess the surface roughness and identify the nano-features of etched or unetched PTC endplates, respectively. The viability, morphology and the expression of specific osteogenic markers were examined after 7 days of culture in the seeded cells. Haralick texture analysis was carried out on the unseeded endplates to correlate surface texture features to the biological data. The acid-etching process modified the surface roughness of the 3D-printed PTC endplates, creating micro- and nano-scale structures that significantly contributed to sustaining the viability of hBM-MSCs and triggering the expression of early osteogenic markers, such as alkaline phosphatase activity and bone-ECM protein production. Finally, the topography of 3D-printed PTC endplates influenced Haralick’s features, which in turn correlated with the expression of two osteogenic markers, osteopontin and osteocalcin. Overall, these data demonstrate that the acid-etching process of PTC endplates created a favourable environment for osteogenic differentiation of hBM-MSCs and may potentially have clinical benefit.

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Haralick's texture analysis to predict cellular proliferation on randomly oriented electrospun nanomaterials

Abstract: Haralick's texture analysis of the biomaterials was used to assess and predict the cell behaviour on a nanomaterial surface.

Cited by 5 publications

References 38 publications

Surface Properties of a Biocompatible Thermoplastic Polyurethane and Its Anti-Adhesive Effect against E. coli and S. aureus

Surface Properties of a Biocompatible Thermoplastic Polyurethane and Its Anti-Adhesive Effect against E. coli and S. aureus

Combined Effects of HA Concentration and Unit Cell Geometry on the Biomechanical Behavior of PCL/HA Scaffold for Tissue Engineering Applications Produced by LPBF

Early Osteogenic Marker Expression in hMSCs Cultured onto Acid Etching-Derived Micro- and Nanotopography 3D-Printed Titanium Surfaces

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