Hard X-ray full-field nanoimaging using a direct photon-counting detector

Flenner, Silja; Hagemann, J.; Wittwer, Felix; Longo, Elena; Kubec, Adam; Rothkirch, André; Dávid, Christian; Müller, Martin; Greving, Imke

doi:10.1107/s1600577522012103

Cited by 5 publications

(2 citation statements)

References 66 publications

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“…For comparison, a standard gold FZP (300 mm diameter) of better quality yields 2000 photons pixel À 1 s À 1 (Flenner et al, 2023). However, the dFZP can, in principle, also be manufactured with the same structure height as conventional FZPs, yielding a comparable efficiency.…”

Section: Resultsmentioning

confidence: 99%

“…The order-sorting apertures are mounted in the focal plane to block the direct beam and higher diffraction orders of the dFZP. An Si-based photon counting Xspectrum LAMBDA 750k with a pixel size of 55 mm is used as the detector (Flenner et al, 2023). This detector consists of 12 individual chips of 256 pixels � 256 pixels arranged on a 2 � 6 grid.…”

Section: Experimental Settingmentioning

confidence: 99%

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Dual-beam X-ray nano-holotomography

Flenner,

Kubec,

David

et al. 2024

J Synchrotron Radiat

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Nanotomography with hard X-rays is a widely used technique for high-resolution imaging, providing insights into the structure and composition of various materials. In recent years, tomographic approaches based on simultaneous illuminations of the same sample region from different angles by multiple beams have been developed at micrometre image resolution. Transferring these techniques to the nanoscale is challenging due to the loss in photon flux by focusing the X-ray beam. We present an approach for multi-beam nanotomography using a dual-beam Fresnel zone plate (dFZP) in a near-field holography setup. The dFZP generates two nano-focused beams that overlap in the sample plane, enabling the simultaneous acquisition of two projections from slightly different angles. This first proof-of-principle implementation of the dual-beam setup allows for the efficient removal of ring artifacts and noise using machine-learning approaches. The results open new possibilities for full-field multi-beam nanotomography and pave the way for future advancements in fast holotomography and artifact-reduction techniques.

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Section: Resultsmentioning

confidence: 99%

Section: Experimental Settingmentioning

confidence: 99%

Dual-beam X-ray nano-holotomography

Flenner,

Kubec,

David

et al. 2024

J Synchrotron Radiat

Self Cite

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Laboratory x-ray nano-computed tomography for biomedical research

Dreier,

Krüger,

Bernström

et al. 2024

J. Inst.

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High-resolution x-ray tomography is a common technique for biomedical research using synchrotron sources. With advancements in laboratory x-ray sources, an increasing number of experiments can be performed in the lab. In this paper, the design, implementation, and verification of a laboratory setup for x-ray nano-computed tomography is presented using a nano-focus x-ray source and high geometric magnification not requiring any optical elements. Comparing a scintillator-based detector to a photon counting detector shows a clear benefit of using photon counting detectors for these applications, where the flux of the x-ray source is limited and samples have low contrast. Sample contrast is enhanced using propagation-based phase contrast. The resolution of the system is verified using 2D resolution charts and using Fourier Ring Correlation on reconstructed CT slices. Evaluating noise and contrast highlights the benefits of photon counting detectors and the contrast improvement through phase contrast. The implemented setup is capable of reaching sub-micron resolution and satisfying contrast in biological samples, like paraffin embedded tissue.

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Dose-efficient in vivo X-ray phase contrast imaging at micrometer resolution by Bragg magnifiers

Spiecker,

Pfeiffer,

Biswal

et al. 2023

Optica

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X-ray imaging enables the study of morphodynamic and physiological processes in living organisms. However, the required photon flux increases with the desired spatial resolution and with it the requirements for dose efficiency. We realize full-field imaging at micrometer resolution close to the highest possible dose efficiency. This is achieved by combining propagation-based phase contrast with Bragg crystal optics and a high-Z single-photon-counting detector, all designed for X-ray energies that allow minimal dose for a given image quality. We prove the superior imaging performance compared to conventional systems and, in particular, show a substantial increase in dose efficiency for high spatial frequencies that comprise the relevant high-resolution components of the image. We demonstrate the potential of the technique by a behavioral in vivo study of submillimeter-sized parasitoid chalcid wasps within their host eggs before and during emergence. The findings show that the technique opens up new possibilities for dose-sensitive studies at micrometer resolution, not only in life sciences but also in materials research.

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Hard X-ray full-field nanoimaging using a direct photon-counting detector

Cited by 5 publications

References 66 publications

Dual-beam X-ray nano-holotomography

Dual-beam X-ray nano-holotomography

Laboratory x-ray nano-computed tomography for biomedical research

Dose-efficient in vivo X-ray phase contrast imaging at micrometer resolution by Bragg magnifiers

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