Hardiness dependent accumulation of phospholipids in leaves of wheat cultivars

Horváth, István; Vı́gh, László; Belea, A.; Farkas, Tibor

doi:10.1111/j.1399-3054.1980.tb02638.x

Cited by 36 publications

(9 citation statements)

References 24 publications

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“…In Miranovskaja the total thylakoid galactolipid level was maintained during hardening and the DGDG fraction was proportionally increased, as was also observed in pine (12 (3). It may be argued that under freezing conditions PC from a cytoplasmic pool is transported to the plasmalemma to replace plasmalemma PC which has been degraded by freezing-induced phospholipase D-activity (5).…”

Section: Resultsmentioning

confidence: 55%

Effect of Frost Hardening on Lipid and Fatty Acid Composition of Chloroplast Thylakoid Membranes in Two Wheat Varieties of Contrasting Hardiness

Vigh¹,

Horvàth²,

Hasselt³

et al. 1985

Plant Physiol.

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Lipid and fatty acid composition of chloroplast thylakoid membranes was determined in two varieties of wheat (Triticum aestivum L.), the hardy Miranovskaja and the sensitive Penjamo. Plants were grown at room temperature or under frost hardening conditions (1.5°C). Changes in lipid and fatty acid composition of the isolated thylakoids could be related to the temperature dependence of light-stimulated proton uptake. Changes in the thylakoid phospholipids upon hardening of the two varieties did not show any direct relation with low temperature tolerance of light-dependent H' uptake; neither did changes in phospholipid fatty acid chain lengthening to 20 and 22 C-atoms in combination with increased desaturation up to 6 double bonds. Increased low temperature tolerance of light-induced H' uptake by hardening was correlated with the following glycolipid changes: maintained glycolipid level, a proportionally increased digalactosyl diglyceride fraction, a decrease in thylakoid monogalactosyl diglyceride, increased sulfolipid fatty acid chain lengthening (20 and 22 C-atoms), and increased sulfolipid desaturation (4-6 double bonds). We suggest that the above mentioned changes in glycolipids have adaptive value for low temperature tolerance of lightdependent proton uptake.proportionally or remained unaffected in some species/varieties (frost-sensitive wheat variety [17]; pea and spinach [1]), whereas in the thylakoids of other species an increased DGDG/MGDG ratio was observed upon hardening (frost-resistant wheat variety, [17]; pine, [12] Growth Conditions and Hardening Procedure. Seeds of two wheat varieties (Triticum aestivum L.), the frost-sensitive Penjamo and the hardy variety Miranovskaja, sown in soil, were germinated at a day/night temperature regime of 20/12C, 16 h d-length, for 6 d. The hardening program was subsequently started by lowering the temperature gradually during 3 weeks, followed by a continuous period of 4 weeks at 1.50C, with the same light regime. After finishing the hardening, the leaves were harvested for thylakoid isolation.For the isolation of thylakoids the procedure of Lineberger and Steponkus (7) was used. Lipids were extracted as described earlier (4)

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Section: Resultsmentioning

confidence: 55%

Effect of Frost Hardening on Lipid and Fatty Acid Composition of Chloroplast Thylakoid Membranes in Two Wheat Varieties of Contrasting Hardiness

Vigh¹,

Horvàth²,

Hasselt³

et al. 1985

Plant Physiol.

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“…Other minor percentage differences between stressed and control shoot mitochondria were detected in various phospholipid species. Horvath (10) showed that low temperature stress altered the content of PC in hardened wheat cultivars and concluded that hardiness resulted from altered PC levels in cellular membranes. Other studies have shown environmental stresses to alter the polar head group composition of cellular membranes, especially the content of PC (3, 9-1 1).…”

Section: Methodsmentioning

confidence: 99%

“…To determine the effect of osmotic stress on lipid metabolism and to determine whether changes in mitochondrial ion transport are associated with changes in membrane lipid composition, the phospholipid and fatty acid composition of root and shoot mitochondria were determined. Environmental stresses have been shown to affect the phospholipid level and composition of plant tissues, and these changes have been related to stress adaptation (3,10,11,25). Often only lipid analyses of whole tissue or organs have been related to adaptation of a given plant species.…”

Section: Methodsmentioning

confidence: 99%

Effect of Osmotic Stress on Ion Transport Processes and Phospholipid Composition of Wheat (Triticum aestivum L.) Mitochondria

Klein

Burke²,

Wilson³

1986

Plant Physiol.

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The effect of osmotic stress on wheat (Triticum aestivum L.) mitochondrial activity and phospholipid composition was investipted. Preliminary growth measurements showed that osmotic stress (-0.25 or -0.5 megapascal external water potential) inhibited the rate of shoot dry matter accumulation while root dry matter accumulation was less sensitive. We have determined that differences in sensitivity to osmotic stress existed between tissues at the mitochondrial level. Mitochondria isolated from roots or shoots of stressed seedlings showed respiratory control and ADP/O ratios similar to control seedlings which indicates that stressed mitochondria were well coupled. However, under passive swelling conditions in a KCI reaction mixture, the rate and extent of valinomycininduced swelling of shoot mitochondria were increased by osmotic stress while root mitochondria were largely unaffected. Active ion transport studies showed efflux transport by stressed-shoot mitochondria to be partially inhibited since mitochondrial contraction required the addition of N-ethylmaleimide or nigericin. Efflux ion transport by root mitochondria was not inhibited by osmotic stress which indicates that stressinduced changes in ion transport were largely limited to shoot mitochondria. Characterization of mitochondrial fatty acid and phospholipid composition showed an increase in the percentage of phosphatidylcholine in stressed shoot mitochondria compared to the control. Mitochondrial fatty acid composition was not markedly altered by stress. No significant changes in either the phospholipid or fatty acid composition of stressed root mitochondria were observed. Hence, these results suggest that a tissue-specific response to osmotic stress exists at the mitochondrial level.Physiological responses of plants to water deficits generally vary with the severity and duration of the stress. The most sensitive processes are altered by a very mild stress and these changes intensify while additional processes become affected in accordance to their sensitivity to the stress (2).Sensitivity to water stress depends on the tissue in question. Under mild water stress, shoot growth is restricted while root growth continues (28,31 (1,15,22, 27), most have been restricted to water stress effects on shoot mitochondria while effects on root mitochondria have been neglected. Studies (1, 27) indicate that mitochondria isolated from air-dried shoots oxidize substrates (proline and to a lesser extent NADH, malate, and succinate) at reduced rates and exhibit generally poor coupling. These results and the lack of osmotic contraction by stmssed mitochondria (22) may indicate the loss ofmembrane integrity due to nonuniform shrinkage of mitochondrial membranes during desiccation (18). It should be emphasized that internal water deficits developed rather rapidly during air drying and became severe in a matter of hours.Because of the paucity of literature in this area, we examined the effect of mild osmotic stress on root and shoot mitochondrial ion and electron tra...

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“…A characteristic feature of winter crops is their ability to adapt to low temperatures, which alter their metabolism considerably. Cold hardening induces accumulation of cryoprotective substances (mainly sugars) and stress proteins, increases the content of unsaturated fatty acids, and decreases the amount of free intracellular water [1][2][3].…”

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confidence: 99%

Response of Winter Wheat to Cold: Production of Phenolic Compounds and L-Phenylalanine Ammonia Lyase Activity

Olenichenko

Загоскина

2005

Appl Biochem Microbiol

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The formation of soluble and polymeric (lignin) phenolic compounds, activity of L-phenylalanine ammonia lyase (PAL, EC 4.3.1.5), and content of free L-phenylalanine during cold hardening of winter wheat plants ( Triticum aestivum L.) were studied. Cold treatment increased accumulation of soluble phenolic compounds in leaves while not affecting the content of lignin. The opposite was observed in tillering nodes. The activity of PAL was lower than in control plants in both tissues, and the content of free L-phenylalanine in tissues increased.

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Hardiness dependent accumulation of phospholipids in leaves of wheat cultivars

Cited by 36 publications

References 24 publications

Effect of Frost Hardening on Lipid and Fatty Acid Composition of Chloroplast Thylakoid Membranes in Two Wheat Varieties of Contrasting Hardiness

Effect of Frost Hardening on Lipid and Fatty Acid Composition of Chloroplast Thylakoid Membranes in Two Wheat Varieties of Contrasting Hardiness

Effect of Osmotic Stress on Ion Transport Processes and Phospholipid Composition of Wheat (Triticum aestivum L.) Mitochondria

Response of Winter Wheat to Cold: Production of Phenolic Compounds and L-Phenylalanine Ammonia Lyase Activity

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