Harmaline exerts potentially anti-cancer effects on U-87 human malignant glioblastoma cells in vitro

Vahedi, Mohammad Mahdi; Shahini, Ali; Mottahedi, Mehran; Garousi, Setareh; Razavi, Seyed Ali Shariat; Pouyamanesh, Ghazaleh; Afshari, Amir R.; Ferns, Gordon A.; Bahrami, Afsane

doi:10.1007/s11033-023-08354-z

Cited by 8 publications

(4 citation statements)

References 34 publications

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“…In a nutshell, the free drugs (N, L), as well as the dual drug-loaded electrospun fiber (N + L@PCL), blocked the MCF-7 cell cycle progression by accumulating the cells in the sub-G1 phase, and the effects were shown to be maximized with the N + L@PCL nanofiber. The findings were in agreement with recent studies involving L and N on glioblastoma and non-small cell lung cancer, respectively [57,58]. All of which confirm our reported results of the apoptosis induction assay.…”

Section: Cell Cycle Analysissupporting

confidence: 93%

“…The observed halting of cell cycle progression at the sub-G1 phase suggests that both drugs can bind to and damage the DNA, resulting in apoptosis [56]. Similar findings were reported for harmaline, which excelled at halting the cell cycle in sub-G1 and promoting apoptosis in U-87 glioblastoma cells [57]. In addition, N treatment resulted in a remarkable increase in the non-small cell lung cancer population in the G 0 /G 1 phase, whereby they interfered with the first stage of the cell cycle [58].…”

Section: Cell Cycle Analysissupporting

confidence: 71%

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Nedaplatin/Peganum harmala Alkaloids Co-Loaded Electrospun, Implantable Nanofibers: A Chemopreventive Nano-Delivery System for Treating and Preventing Breast Cancer Recurrence after Tumorectomy

Sedky,

Arafa,

Abdelhady

et al. 2023

Pharmaceutics

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Currently, the main pillars in treating breast cancer involve tumorectomy pursued by hormonal, radio, or chemotherapies. Nonetheless, these approaches exhibit severe adverse effects and might suffer from tumor recurrence. Therefore, there is a considerable demand to fabricate an innovative controlled-release nano-delivery system to be implanted after tumor surgical removal to guard against cancer recurrence. In addition, combining platinum-based drugs with phytochemicals is a promising approach to improving the anticancer activity of the chemotherapeutics against tumor cells while minimizing their systemic effects. This study designed polycaprolactone (PCL)-based electrospun nanofiber mats encapsulating nedaplatin (N) and Peganum harmala alkaloid-rich fraction (L). In addition to physicochemical characterization, including average diameters, morphological features, degradation study, thermal stability, and release kinetics study, the formulated nanofibers were assessed in terms of cytotoxicity, where they demonstrated potentiated effects and higher selectivity towards breast cancer cells. The dual-loaded nanofiber mats (N + L@PCL) demonstrated the highest antiproliferative effects against MCF-7 cells with a recorded IC50 of 3.21 µg/mL, as well as the topmost achieved selectivity index (20.45) towards cancer cells amongst all the tested agents (N, L, N@PCL, and L@PCL). This indicates that the dual-loaded nanofiber excelled at conserving the normal breast epithelial cells (MCF-10A). The combined therapy, N + L@PCL treatment, resulted in a significantly higher percent cell population in the late apoptosis and necrosis quartiles as compared to all other treatment groups (p-value of ≤0.001). Moreover, this study of cell cycle kinetics revealed potentiated effects of the dual-loaded nanofiber (N + L@PCL) at trapping more than 90% of cells in the sub-G1 phase and reducing the number of cells undergoing DNA synthesis in the S-phase by 15-fold as compared to nontreated cells; hence, causing cessation of the cell cycle and confirming the apoptosis assay results. As such, our findings suggest the potential use of the designed nanofiber mats as perfect implants to prevent tumor recurrence after tumorectomy.

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Section: Cell Cycle Analysissupporting

confidence: 93%

Section: Cell Cycle Analysissupporting

confidence: 71%

Nedaplatin/Peganum harmala Alkaloids Co-Loaded Electrospun, Implantable Nanofibers: A Chemopreventive Nano-Delivery System for Treating and Preventing Breast Cancer Recurrence after Tumorectomy

Sedky,

Arafa,

Abdelhady

et al. 2023

Pharmaceutics

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“…verified that PH demonstrated antiproliferative effects in human lung cancer cells (A549). Other studies have shown similar results in gastric cancer cells [25,26], breast cancer cell lines (MDA-MB-231 and MCF-7) [23], oesophageal squamous cell carcinoma [39], NSCLC [29,40], glioblastoma [30], carcinoma (Med-mek and UCP-Med) and sarcoma (UCP-Med and Sp2/O-Ag14) [41,42] and human colorectal carcinoma (SW620) [28].…”

Section: Ayahuasca Extracts Affected Oxidative Damage and Activity Of...supporting

confidence: 61%

“…Similarly, it was found that the same compound induced apoptosis in B16F-10 melanoma cells [ 27 ], in human colorectal carcinoma SW620 cells [ 28 ] and in non-small cell lung cancer (NSCLC) cells [ 29 ]. Otherwise, harmaline, present in PH, was responsible for arresting the cell cycle and inducing apoptosis in the glioblastoma cell line [ 30 ]. Other studies were carried out, where only the anticancer effects of synthetic or isolated β-carbolines were evaluated.…”

Section: Resultsmentioning

confidence: 99%

The Role of Ayahuasca in Colorectal Adenocarcinoma Cell Survival, Proliferation and Oxidative Stress

Gonçalves,

Feijó,

Socorro

et al. 2024

Pharmaceuticals

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The psychedelic beverage ayahuasca is originally obtained by Banisteriopsis caapi (B. caapi) (BC) and Psychotria viridis (P. viridis) (PV). However, sometimes these plant species are replaced by others that mimic the original effects, such as Mimosa hostilis (M. hostilis) (MH) and Peganum harmala (P. harmala) (PH). Its worldwide consumption and the number of studies on its potential therapeutic effects has increased. This study aimed to evaluate the anticancer properties of ayahuasca in human colorectal adenocarcinoma cells. Thus, the maximum inhibitory concentration (IC50) of decoctions of MH, PH, and a mixture of these (MHPH) was determined. The activities of caspases 3 and 9 were evaluated, and the cell proliferation index was determined through immunocytochemical analysis (Ki-67). Two fluorescent probes were used to evaluate the production of oxidative stress and the activity of the antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx) was also evaluated. It was demonstrated that exposure to the extracts significantly induced apoptosis in Caco-2 cells, while decreasing cell proliferation. MH and MHPH samples significantly reduced oxidative stress and significantly increased glutathione peroxidase activity. No significant differences were found in SOD activity. Overall, it was demonstrated that the decoctions have a potential anticancer activity in Caco-2 cells.

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Cytotoxicity of alkaloids isolated from Peganum harmala seeds on HCT116 human colon cancer cells

Salimizadeh,

Enferadi,

Majidizadeh

et al. 2024

Mol Biol Rep

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Harmaline exerts potentially anti-cancer effects on U-87 human malignant glioblastoma cells in vitro

Cited by 8 publications

References 34 publications

Nedaplatin/Peganum harmala Alkaloids Co-Loaded Electrospun, Implantable Nanofibers: A Chemopreventive Nano-Delivery System for Treating and Preventing Breast Cancer Recurrence after Tumorectomy

Nedaplatin/Peganum harmala Alkaloids Co-Loaded Electrospun, Implantable Nanofibers: A Chemopreventive Nano-Delivery System for Treating and Preventing Breast Cancer Recurrence after Tumorectomy

The Role of Ayahuasca in Colorectal Adenocarcinoma Cell Survival, Proliferation and Oxidative Stress

Cytotoxicity of alkaloids isolated from Peganum harmala seeds on HCT116 human colon cancer cells

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