Harmful effects of UVA on the structure and barrier function of engineered human cutaneous tissues

Robert, M.; Bissonauth, Vickram; Ross, Geneviève; Rouabhia, Mahmoud

doi:10.1080/095530099140492

Cited by 17 publications

(17 citation statements)

References 32 publications

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“…Epidermal lipids were extracted as previously described with some modifications (Robert et al, 1999). Lipid extracts (1 g per conditions) were analyzed by high-performance thin-layer chromatography (HPTLC) using the total lipid and the ceramide development systems (Ponec and Weerheim, 1990).…”

Section: Epidermal Lipid Extraction and Analysismentioning

confidence: 99%

Effects of all-trans retinoic acid on UVB-irradiated human skin substitute

Chouinard

Rouabhia

1999

J. Cell. Physiol.

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Retinoids are frequently used for treatment of photodamaged skin. We wished to find out whether photodamage could be attenuated by applying all-trans retinoic acid (RA) during repetitive irradiation. For this purpose, we used human cutaneous cells and tissue: pure monolayer cultures containing either keratinocytes or fibroblasts, and human skin substitute (SS) containing both cell types. All cultures were exposed to 8 mJ/cm(2) of UVB and were immediately treated with RA (0, 1.5, or 3 microM). The irradiation and RA treatment protocol was repeated until the cells of the nonirradiated culture had reached confluence. In the irradiated SS, RA preserved the structure (epidermal stratification and differentiation) and ultrastructure (well-organized intermediate filaments and desmosomes) in a state comparable to that observed in nonirradiated SS. As well RA maintained secretion of basement membrane components (laminin and type-IV collagen). Following irradiation, cutaneous cells also displayed more proliferative capacity when SS was treated. In the irradiated monolayer cultures, RA maintained the proliferative capacity of fibroblasts and decreased their differentiation whereas the opposite effect was seen on keratinocytes. In conclusion, RA clearly helps protect human skin against photodamage induced by repeated exposure to UVB.

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Section: Epidermal Lipid Extraction and Analysismentioning

confidence: 99%

Effects of all-trans retinoic acid on UVB-irradiated human skin substitute

Chouinard

Rouabhia

1999

J. Cell. Physiol.

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“…However, a promising alternative to study in animal models is the use of skin equivalent models. In this study, we used skin equivalent models, which are a non-transformed, human-derived, metabolically active, 3 dimensional organotypic in vitro skin model featuring the epidermis Companies have used this model to analyze their products, including performing skin irritation tests [6]. According to a European study of in vitro alternatives for measuring skin irritation potential, EpiDerm received a coefficient correlation of 0.94 [7].…”

Section: Introductionmentioning

confidence: 99%

Investigating the protective properties of milk phospholipids against ultraviolet light exposure in a skin equivalent model

et al. 2010

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Current research on bioactive molecules in milk has documented health advantages of bovine milk and its components. Milk Phospholipids, selected for this study, represent molecules with great potential benefit in human health and nutrition. In this study we used confocal reflectance and multiphoton microscopy to monitor changes in skin morphology upon skin exposure to ultraviolet light and evaluate the potential of milk phospholipids in preventing photodamage to skin equivalent models. The results suggest that milk phospholipids act upon skin cells in a protective manner against the effect of ultraviolet (UV) radiation. Similar results were obtained from MTT tissue viability assay and histology.

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“…Also, the results are more applicable than animal samples due to biological differences that exist being the species. 44 Our study compared the images and data of four different categories of skin specimens at three separate time intervals for a total of 12 samples. Each sample was either; a) unexposed to UV light, b) exposed to UV light, c) fed milk phospholipids or d) …”

Section: Section 32 Alternativesmentioning

confidence: 99%

“…Even though the relationship of exposure to solar UVR and the risk of malignant melanoma is not well understood, there is still strong evidence that the UVA and UVB components of sunlight plays a significant part in the wrinkling and aging of skin and, in more serious cases, the shorter wave radiation of UVB can be absorbed directly by DNA temporarily inhibiting synthesis of new DNA and creating targets for mutagenesis. 44 Sunburn is an acute inflammatory reaction that follows excessive exposure of the skin to ultraviolet radiation (UVR) and is generally classified as a superficial or firstdegree burn. The minimal erythemal dose (MED) for a given skin type is the approximate dosage of solar UVR required to obtain noticeable swelling and reddening of the skin in reaction to UV sun damage.…”

Section: Section 422 Ultraviolet Irradiationmentioning

confidence: 99%

“…UV light is harmful to human skin in its ability to penetrate the epidermis and the dermis reaching the dermal papillae of the hair and the vascular network in the deeper layers of the dermis. 44 The UVR component itself has been subdivided into three separate categories based on wavelength, UVA (315-400nm), UVB (280-315nm), and UVC (100-280nm) with the high-energy UVC range being capable of causing the most damage to mammalian skin. 45 Fortunately, all UVC and a substantial proportion (70-90%) of UVB are prevented by the ozone layer from reaching the earth's surface.…”

Section: Section 422 Ultraviolet Irradiationmentioning

confidence: 99%

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Laser Scanning Confocal Microscopy (Lscm): An Application for the Detection of Morphological Alterations in Skin Structure

Smith¹

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Laser scanning confocal microscopy (LSCM) is an optical imaging technique that provides improved resolution and sensitivity over conventional methods of optical microscopy. However, the cost of most commercial LSCM systems exceeds the financial limitations of many smaller laboratories. The design of a custom LSCM created at a fraction of the cost of a commercial model is discussed in this paper.The increase in the incidence rate of skin cancer in the world today is alarming, as such, it is essential to provide an early, rapid and effective method for in vivo diagnostics of human skin tissue. LSCM is capable of detecting alterations in skin morphology and configuration, as well as providing chemical composition information which may be indicative of the development of skin cancer. If developed successfully, LSCM could replace the current invasive biopsy procedures performed today with a quick, noninvasive optical scanning method that would prove beneficial for both patients and physicians alike.

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Harmful effects of UVA on the structure and barrier function of engineered human cutaneous tissues

Cited by 17 publications

References 32 publications

Effects of all-trans retinoic acid on UVB-irradiated human skin substitute

Effects of all-trans retinoic acid on UVB-irradiated human skin substitute

Investigating the protective properties of milk phospholipids against ultraviolet light exposure in a skin equivalent model

Laser Scanning Confocal Microscopy (Lscm): An Application for the Detection of Morphological Alterations in Skin Structure

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