Harmonic generation in “non-linear” biological systems

Hutchings, M.J.; Blake-Coleman, B.C.; Silley, P.

doi:10.1016/0956-5663(94)80099-5

Cited by 17 publications

(13 citation statements)

References 13 publications

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“…Our findings are partially consistent with the hypothesis of Blake-Coleman and co-workers, [13,14] where the interface region is responsible for the generation of harmonics, and it can be altered by the presence of biological cells. It has been proved that cell presence can modify the linear and non-linear properties of the interface region [32][33][34].…”

Section: Discussionsupporting

confidence: 92%

Collision-Induced Infrared Absorption by Molecular Hydrogen Pairs at Thousands of Kelvin

Hunt

Wang

et al. 2016

Spectroscopy

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Background: Non-linear dielectric spectroscopy (NLDS) of microorganism was characterized by the generation of harmonics in the polarization current when a microorganism suspension was exposed to a sinusoidal electric field. The biological nonlinear response initially described was not well verified by other authors and the results were susceptible to ambiguous interpretation. In this paper NLDS was performed to yeast suspension in tripolar and tetrapolar configuration with a recently developed analyzer.

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Section: Discussionsupporting

confidence: 92%

Collision-Induced Infrared Absorption by Molecular Hydrogen Pairs at Thousands of Kelvin

Hunt

Wang

et al. 2016

Spectroscopy

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“…The modulated transmembrane potential drives membrane proteins to change their conformational states, and can even induce pumps to transport ions [6]. Protein conformational changes and ion translocation create a nonlinear response manifested by the generation of harmonics [7], [8]. An externally modulated membrane potential may therefore affect enzyme activity, transport, and conformational behavior.…”

Section: Introductionmentioning

confidence: 99%

SQUID-Based Biosensor for Probing Ion Transporters in Cell Suspensions and Tissue

Nawarathna

Claycomb

Cárdenas

et al. 2007

IEEE Trans. Appl. Supercond.

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We have developed a SQUID-based technique to probe membrane-bound proteins, such as cation transporters. The method involves excitation of a biological sample with a sinusoidal electric field (0-5 V/cm, 10-300 Hz), detection, with a SQUID magnetometer, of the magnetic field produced by ac currents in the tissue or cell suspension, and calculation of the Fast Fourier Transform (FFT) of the measured response to obtain a spectrum. We find that the harmonic response of budding yeast cells (S. cerevisiae, 10 8 cells/ml) is sensitive to sodium metavanadate, an inhibitor of a proton pump known as H + -ATPase, glucose, a substrate of H + -ATPase, and gramicidin, a membrane depolarizer. SQUID-based field and frequency dependent harmonic response measurements are also made of a live earthworm (Lumbricus terrestris). Use of the SQUID significantly reduces spurious harmonics and distortion of the excitation field by the measurement electrodes.

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“…Over the same period, the group of Blake-Coleman tried to repeat and improve these findings, but their results did not fully agree with the previous study [8]. This publication supported the theory that yeast does not show evidence of nonlinearities within the applied voltage and frequency ranges; it rather affects the intrinsic behavior of the interface (EEI).…”

Section: Introductionmentioning

confidence: 86%

Non linear dielectric properties of microbiological suspensions at electrode-electrolyte interfaces

Treo

Felice

Madrd

2005

2005 IEEE Engineering in Medicine and Biology 27th Annual Conference

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Nonlinear properties of biological suspensions have been previously presented as a bulk phenomenon, however, some authors consider that they are generated at the electrode-electrolyte interface and are reflected as a bulk property phenomenon. They were mainly ascribed to the H⁺-ATPase present in the plasma membrane of these cells. In this paper, we describe the construction of a dual-cell nonlinear dielectric spectrometer. The system is applied to the study of interfaces with resting cell suspensions of Saccharomyces cerevisiae. Substantial harmonics are generated at the interface when a sinusoidal current is applied and altered by the presence of yeast. When a second interface with yeast and enzyme inhibitor is used as reference, a decrease in the magnitude response of the third harmonic can be observed. As it was already described for the solution bulk, we obtained a similar behavior at the interface. Besides, we also found optimal intervals, respectively, for frequency and voltage to reach maximal response. This result would support the contention that biological nonlinearity is an interface-based phenomenon.

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Harmonic generation in “non-linear” biological systems

Cited by 17 publications

References 13 publications

Collision-Induced Infrared Absorption by Molecular Hydrogen Pairs at Thousands of Kelvin

Collision-Induced Infrared Absorption by Molecular Hydrogen Pairs at Thousands of Kelvin

SQUID-Based Biosensor for Probing Ion Transporters in Cell Suspensions and Tissue

Non linear dielectric properties of microbiological suspensions at electrode-electrolyte interfaces

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