This review presents the main results of a 10-year research study conducted in a Mediterranean intermittent basin (Evrotas River). By assembling the main outcomes of past and ongoing research projects, this study provides an overview of multiple stressor effects, with emphasis on water scarcity, focusing on hydro-biogeochemical processes, as well as on spatial and temporal variations in benthic macroinvertebrates and fish fauna. The major impact in the basin has been the over-exploitation of surface and groundwater resources, which, in combination with droughts, has resulted in the recurrent artificial desiccation of large parts of the hydrological network. The response to intermittency of the macroinvertebrate fauna is characterized by high resilience through various drought-resistant evolutionary mechanisms, with assemblages recovering successfully after recurrent droughts. However, when pollution is evident in combination with drought, effects on benthic species richness, abundance, and assemblage structure can be severe. Similarly, pollution and water stress may result in massive fish mortalities due to hypoxic conditions, with fish populations requiring long periods to recover. However, the fish fauna appears to be relatively resilient to drought-driven reach-scale desiccation, and ultimately recovers, provided that aquatic refugia are available to supply colonists and that there are no physical barriers impeding recolonisation.Appropriate conservation measures are urgently required to address the effects of recurrent bouts of water stress, as well as of other stressors on the freshwater communities of the Evrotas River, both at the level of water management and of water policy and at the local and the national level. It has long been acknowledged that such extreme climatic conditions of water scarcity and prolonged droughts influence both the physicochemical and the ecological status of water bodies (Barceló & Sabater, 2010). Extended low-flow periods result in increased water temperatures, which may rapidly equilibrate with the ambient terrestrial environment and cause modified evaporation patterns (Hamilton, Bunn, Thoms, & Marshall, 2005) and irregular pool-riffle succession.
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