2019
DOI: 10.1080/21505594.2019.1706913
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Harnessing efficient multiplex PCR methods to detect the expanding Tet(X) family of tigecycline resistance genes

Abstract: A growing number of tet(X)-type tigecycline resistance determinants [tet(X1) to tet(X5)] constitutes an expanding family of tetracycline-inactivating enzymes, posing a potential risk to global public health. Here, we report the development of an efficient multiplex PCR method to detect the family of tet(X) variants. This method is successfully applied in the screen and validation of tet(X) genes in the field and clinic bacterial samples. In addition, we found that the formerly proposed tet(X1) is a premature t… Show more

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Cited by 28 publications
(21 citation statements)
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“…These strains were designated GX3, GX5, and GX7. We screened for the tigecycline-resistant genes tet (X3), tet (X4), and tet (X5) with PCR, as described in a previous report ( Ji et al., 2020 ).…”
Section: Methodsmentioning
confidence: 99%
“…These strains were designated GX3, GX5, and GX7. We screened for the tigecycline-resistant genes tet (X3), tet (X4), and tet (X5) with PCR, as described in a previous report ( Ji et al., 2020 ).…”
Section: Methodsmentioning
confidence: 99%
“…The plasmid-encoded tet(X1 to X5) genes conferring TIG resistance were amplified using multiplex PCR (Ji et al, 2020). The presence of tet(A), tet(B), and tet(M) genes were also examined (Van et al, 2008) using primer sequences depicted in Supplementary Table 1.…”
Section: Detection Of Tetracycline Resistance Determinantsmentioning
confidence: 99%
“…Tetracyclines are a collection of wide-spectrum antibiotics, exhibiting appreciable antibacterial activity against varieties of Gram-negative and Gram-positive bacteria (Ji et al, 2020). Given a number of advantages (including low cost, few side effects, excellent antibacterial activity, and oral administration), they are widely used in the treatment of bacterial infectious diseases in clinic and agriculture.…”
Section: Overview Of Tetracyclines and The Resistance Mechanismsmentioning
confidence: 99%
“…Tet(X) and its homologue belong to the class A FMOs family and appear to be the flagship of the tetracycline destroying enzymes (Ji et al, 2020). Although Tet(X) has been shown to inactivate tigecycline in certain aerobic Gram-negative bacteria (Fang et al, 2020;Leski et al, 2013), Tet(X)-mediated tigecycline resistance was poorly addressed in clinic sector.…”
Section: The Emergence Of New Tet(x) Variantmentioning
confidence: 99%