Harnessing Growth Factors to Influence Wound Healing

Kiwanuka, Elizabeth; Junker, Johan P.E.; Eriksson, Elof

doi:10.1016/j.cps.2012.04.003

Cited by 62 publications

(46 citation statements)

References 93 publications

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“…However, differences observed in total cytokine release between different stages of healing and between non-diabetic and diabetic mice at least qualitatively reflect levels measured in total wound homogenates reported previously by us and others [1, 3, 4, 7, 11]. Another limitation is that cells were grouped into mixed cell subsets and thus the contributions of individual cell types were not determined within each subset.…”

Section: Resultsmentioning

confidence: 76%

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Contributions of cell subsets to cytokine production during normal and impaired wound healing

Mirza

Koh

2015

Cytokine

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The objective of this study was to determine the relative contributions of different cell subsets to the production of cytokines and growth factors during normal and impaired wound healing. Cells were isolated from wounds of non-diabetic and diabetic mice and separated by magnetic sorting into neutrophils/T cells/B cells (NTB cell subset), monocytes/macrophages (Mo/Mp subset) and non-leukocytic cells including keratinocyte/fibroblast/endothelial cells (KFE subset). On both per cell and total contribution bases, the Mo/Mp subset was the dominant producer of pro-inflammatory cytokines interleukin (IL)-1β, tumor necrosis factor (TNF)-α and IL-6 in both non-diabetic and diabetic mice and was a significant producer of vascular endothelial cell growth factor (VEGF)-A, insulin-like growth factor (IGF)-1 and transforming growth factor (TGF)-β1. The NTB subset was also a significant producer of TNF-α and IL-10 whereas the KFE subset contributed significant amounts of VEGF, IGF-1 and TGF-β1. Sustained production of pro-inflammatory cytokines and impaired production of healing-associated factors were evident in each subset in diabetic mice. These data will be useful for further experimental and modeling studies on the role of cell subsets in wound healing as well as for designing therapeutic strategies for improving healing.

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Section: Resultsmentioning

confidence: 76%

“…The majority of these studies involve either immunohistochemical assessment of tissue sections or cell culture studies using primary cells or cell lines [1, 3, 4, 11]. However, these methods are not optimal for determining the relative contribution of cell subsets to wound cytokine levels.…”

Section: Introductionmentioning

confidence: 99%

Contributions of cell subsets to cytokine production during normal and impaired wound healing

Mirza

Koh

2015

Cytokine

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“…The VEGF is produced by a variety of cell types during wound healing, and is a potent stimulator of proliferation and migration in endothelial cells [15–17]. The expression of VEGF was significantly increased compared to the control group at 7 days after wound (16.6 ± 0.7 versus 12.9 ± 2.9 pg/mg, P < 0.05) (Figure 5(h)).…”

Section: Resultsmentioning

confidence: 99%

Therapeutic Effects of Acupuncture through Enhancement of Functional Angiogenesis and Granulogenesis in Rat Wound Healing

Park

Sunwoo

Jung

et al. 2012

Evidence-Based Complementary and Alternative Medicine

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Acupuncture regulates inflammation process and growth factors by increasing blood circulation in affected areas. In this study, we examined whether acupuncture has an effect on wound healing in injured rat. Rats were assigned randomly into two groups: control group and acupuncture group. Acupuncture treatment was carried out at 8 sites around the wounded area. We analyzed the wound area, inflammatory cytokines, proliferation of resident cells, and angiogenesis and induction of extracelluar matrix remodeling. At 7 days after-wounding the wound size in acupuncture-treat group was decreased more significantly compared to control group. In addition, the protein levels of proinflammatory cytokines such as tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were significantly decreased compared to the control at 2 and 7 days post-wounding. Also, we analyzed newly generated cells by performing immunostaining for PCNA and using several phenotype markers such as CD-31, α-SMA, and collagen type I. In acupuncture-treated group, PCNA-positive cell was increased and PCNA labeled CD-31-positive vessels, α-SMA- and collagen type I-positive fibroblastic cells, were increased compared to the control group at 7 days post-wounding. These results suggest that acupuncture may improve wound healing through decreasing pro-inflammatory response, increasing cell proliferation and angiogenesis, and inducing extracellular matrix remodeling.

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“…The role of growth factors and cytokines in the fibroblast during wound healing has been discussed in other excellent reviews. [22][23][24][25] Since its manipulation and augmentation is emerging in clinical treatment (including use of acellular dermal matrices and topical wound dressings), the influence of the ECM on the fibroblast, both mechanically and through modulation of molecular pathways, warrants further discussion.…”

Section: -21mentioning

confidence: 99%