Harnessing metal ion affinity for the purification of plasmid DNA

Monolith-based immobilized metal affinity chromatography increases production efficiency for plasmid DNA purification

“…In our previous study, CuCl 2 -induced precipitation followed by Cu 2+ -iminodiacetic acid (IDA) based immobilized metal affinity chromatography (IMAC) were conducted to purify pDNA from its contaminants in the batch binding mode [17]. In brief, pDNA, RNA and endotoxin were precipitated by free Cu 2+ ions from alkaline cell lysate while the majority of other impurities remain in the supernatant.…”

Removal of endotoxins from plasmid DNA: Analysis of aggregative interaction of mobile divalent metal cations with endotoxins and plasmid DNA

“…One of the major concerns on cation‐induced pDNA purification is binding of cations on pDNA molecules, which could affect its yield as well as biological functionality. A study, however, has found that treatment with EDTA could potentially resolubilise pDNA back into its native form leading to high yield and enhancing further downstream processes .…”

A new strategy for the on-column exopeptidase cleavage of poly-histidine tagged proteins

“…In fact, ever since the introduction of poly-histidine fusion tags and IMAC, the technology has gained popularity as a platform technology capable of being incorporated into most existing downstream flowsheets [1,2]. In addition, IMAC adsorbents can be easily regenerated and retain adsorption capacity and robustness following multiple cycles of cleaning and reuse, giving consistent success across all scales from laboratory preparation to manufacturing [3][4][5][6][7].…”