Harnessing the hydrolytic potential of phytopathogenic fungus Phoma exigua ITCC 2049 for saccharification of lignocellulosic biomass

Tiwari, Rameshwar; Singh, Surender; Nain, Pawan Kumar Singh; Rana, Sarika; Sharma, Anamika; Pranaw, Kumar; Nain, Lata

doi:10.1016/j.biortech.2013.10.007

Cited by 22 publications

(11 citation statements)

References 29 publications

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“…These were approximately consistent with results of enzymes activities detection. Analyses indicated that the secretome of P. ananatis Sd-1 release higher amounts of reducing sugars than that reported for Phoma exigua secretome which used pretreated paddy straw or wheat straw as substrates [ 42 , 43 ]. These indicated that P. ananatis Sd-1 is deserved an in-depth exploration for its enzyme system, such as GHs and CEs.…”

Section: Discussionmentioning

confidence: 99%

Genomic and secretomic insight into lignocellulolytic system of an endophytic bacterium Pantoea ananatis Sd-1

Zhang

Liao

et al. 2016

Biotechnol Biofuels

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BackgroundExploring microorganisms especially bacteria associated with the degradation of lignocellulosic biomass shows great potentials in biofuels production. The rice endophytic bacterium Pantoea ananatis Sd-1 with strong lignocellulose degradation capacity has been reported in our previous study. However, a comprehensive analysis of its corresponding degradative system has not yet been conducted. The aim of this work is to identify and characterize the lignocellulolytic enzymes of the bacterium to understand its mechanism of lignocellulose degradation and facilitate its application in sustainable energy production.ResultsThe genomic analysis revealed that there are 154 genes encoding putative carbohydrate-active enzymes (CAZy) in P. ananatis Sd-1. This number is higher than that of compared cellulolytic and ligninolytic bacteria as well as other eight P. ananatis strains. The CAZy in P. ananatis Sd-1 contains a complete repertoire of enzymes required for cellulose and hemicellulose degradation. In addition, P. ananatis Sd-1 also possesses plenty of genes encoding potential ligninolytic relevant enzymes, such as multicopper oxidase, catalase/hydroperoxidase, glutathione S-transferase, and quinone oxidoreductase. Quantitative real-time PCR analysis of parts of genes encoding lignocellulolytic enzymes revealed that they were significantly up-regulated (at least P < 0.05) in presence of rice straw. Further identification of secretome of P. ananatis Sd-1 by nano liquid chromatography–tandem mass spectrometry confirmed that considerable amounts of proteins involved in lignocellulose degradation were only detected in rice straw cultures. Rice straw saccharification levels by the secretome of P. ananatis Sd-1 reached 129.11 ± 2.7 mg/gds. Correspondingly, the assay of several lignocellulolytic enzymes including endoglucanase, exoglucanase, β-glucosidase, xylanase-like, lignin peroxidase-like, and laccase-like activities showed that these enzymes were more active in rice straw relative to glucose substrates. The high enzymes activities were not attributed to bacterial cell densities but to the difference of secreted protein contents.ConclusionOur results indicate that P. ananatis Sd-1 can produce considerable lignocellulolytic enzymes including cellulases, hemicellulases, and ligninolytic relevant enzymes. The high activities of those enzymes could be efficiently induced by lignocellulosic biomass. This identified degradative system is valuable for the lignocellulosic bioenergy industry.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-016-0439-8) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Genomic and secretomic insight into lignocellulolytic system of an endophytic bacterium Pantoea ananatis Sd-1

Zhang

Liao

et al. 2016

Biotechnol Biofuels

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“…Samples were taken from the reaction mixture at different time intervals and centrifuged at 10000 rpm for 5 min. The supernatant was used for analysis of reducing sugar by HPLC as described previously [14] using Waters HPLC and Aminex HPX-87H column. Saccharification efficiency was calculated by the following formula as described by NREL [13]:

\begin{matrix} Saccharification (%) \\ = \frac{Reducing sugars released (mg) \times 0.9}{Carbohydrate content in pretreated biomass} \times 100 . \end{matrix}

…”

Section: Methodsmentioning

confidence: 99%

Optimization of Enzymatic Saccharification of Alkali Pretreated Parthenium sp. Using Response Surface Methodology

et al. 2014

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Parthenium sp. is a noxious weed which threatens the environment and biodiversity due to its rapid invasion. This lignocellulosic weed was investigated for its potential in biofuel production by subjecting it to mild alkali pretreatment followed by enzymatic saccharification which resulted in significant amount of fermentable sugar yield (76.6%). Optimization of enzymatic hydrolysis variables such as temperature, pH, enzyme, and substrate loading was carried out using central composite design (CCD) in response to surface methodology (RSM) to achieve the maximum saccharification yield. Data obtained from RSM was validated using ANOVA. After the optimization process, a model was proposed with predicted value of 80.08% saccharification yield under optimum conditions which was confirmed by the experimental value of 85.80%. This illustrated a good agreement between predicted and experimental response (saccharification yield). The saccharification yield was enhanced by enzyme loading and reduced by temperature and substrate loading. This study reveals that under optimized condition, sugar yield was significantly increased which was higher than earlier reports and promises the use of Parthenium sp. biomass as a feedstock for bioethanol production.

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“…Various substrates like Sigmacell 101, CMC, α-cellulose and Avicell PH101 have been used for induction of holocellulolytic enzymes [23]. Ribosomal DNA based identification revealed that all isolates belong to the phylum Ascomycota (Fig.…”

Section: Discussionmentioning

confidence: 99%

Cold active holocellulase cocktail from Aspergillus niger SH3: process optimization for production and biomass hydrolysis

Tiwari

Nain

Singh

et al. 2015

Journal of the Taiwan Institute of Chemical Engineers

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Harnessing the hydrolytic potential of phytopathogenic fungus Phoma exigua ITCC 2049 for saccharification of lignocellulosic biomass

Cited by 22 publications

References 29 publications

Genomic and secretomic insight into lignocellulolytic system of an endophytic bacterium Pantoea ananatis Sd-1

Genomic and secretomic insight into lignocellulolytic system of an endophytic bacterium Pantoea ananatis Sd-1

Optimization of Enzymatic Saccharification of Alkali Pretreated Parthenium sp. Using Response Surface Methodology

Cold active holocellulase cocktail from Aspergillus niger SH3: process optimization for production and biomass hydrolysis

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