Harnessing the Power of Enteric Glial Cells’ Plasticity and Multipotency for Advancing Regenerative Medicine

Lefèvre, Marie A.; Soret, Rodolphe; Pilon, Nicolas

doi:10.3390/ijms241512475

Cited by 3 publications

(2 citation statements)

References 151 publications

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“…13 This study found that the levels of GFAP and GDNF were lower in UC mice compared to the normal group, indicating impaired status and function of EGCs in UC, which is consistent with previous studies. 29,30 So, in the future, whether it is possible to use levels of EGCs and other related signals, such as GDNF or p-RET, as markers of intestinal health is a question worth exploring in depth. Additionally, we validated the role of GDNF in this study.…”

Section: Discussionmentioning

confidence: 99%

Glycyrol Relieves Ulcerative Colitis by Promoting the Fusion of ZO-1 with the Cell Membrane through the Enteric Glial Cells GDNF/RET Pathway

Lu,

Xu,

Zhang

et al. 2024

J. Agric. Food Chem.

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The damage to the mechanical barrier of the intestinal mucosa is the initiating factor and the core link of the progression of ulcerative colitis (UC). Protecting the mechanical barrier of the intestinal mucosa is of great significance for improving the health status of UC patients. ZO-1 is a key scaffold protein of the mechanical barrier of the intestinal mucosa, and its fusion with the membrane of the intestinal epithelium is a necessary condition to maintain the integrity of the mechanical barrier of the intestinal mucosa. Enteric glial cells (EGCs) play an important role in the maintenance of intestinal homeostasis and have become a new target for regulating intestinal health in recent years. In this study, we found that glycyrol (GC), a representative coumarin compound isolated from Licorice (Glycyrrhiza uralensis Fisch, used for medicine and food), can alleviate UC by promoting the production of neurotrophic factor GDNF in mice EGCs. Specifically, we demonstrated that GC promotes the production of GDNF, then activates its receptor RET, promotes ZO-1 fusion with cell membranes, and protects the intestinal mucosal mechanical barrier. The results of this study can provide new ideas for the prevention and treatment of UC.

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Section: Discussionmentioning

confidence: 99%

Glycyrol Relieves Ulcerative Colitis by Promoting the Fusion of ZO-1 with the Cell Membrane through the Enteric Glial Cells GDNF/RET Pathway

Lu,

Xu,

Zhang

et al. 2024

J. Agric. Food Chem.

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“…Once NCCs enter the gut mesenchyme, at which point they are referred to as enteric neural-crest-derived cells (ENCDCs), they migrate along the length of the entire GI tract, to form the enteric nervous system (ENS) [2,3,5,7]. After gut colonization by vagal and sacral ENCDCs, Schwann cell precursors enter the gut alongside the extrinsic nerves [9][10][11][12][13][14][15]. Abnormal ENS formation Int.…”

Section: Introductionmentioning

confidence: 99%

Essential Role of BMP4 Signaling in the Avian Ceca in Colorectal Enteric Nervous System Development

Kovács,

Halasy,

Pethő

et al. 2023

IJMS

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The enteric nervous system (ENS) is principally derived from vagal neural crest cells that migrate caudally along the entire length of the gastrointestinal tract, giving rise to neurons and glial cells in two ganglionated plexuses. Incomplete migration of enteric neural crest-derived cells (ENCDC) leads to Hirschsprung disease, a congenital disorder characterized by the absence of enteric ganglia along variable lengths of the colorectum. Our previous work strongly supported the essential role of the avian ceca, present at the junction of the midgut and hindgut, in hindgut ENS development, since ablation of the cecal buds led to incomplete ENCDC colonization of the hindgut. In situ hybridization shows bone morphogenetic protein-4 (BMP4) is highly expressed in the cecal mesenchyme, leading us to hypothesize that cecal BMP4 is required for hindgut ENS development. To test this, we modulated BMP4 activity using embryonic intestinal organ culture techniques and retroviral infection. We show that overexpression or inhibition of BMP4 in the ceca disrupts hindgut ENS development, with GDNF playing an important regulatory role. Our results suggest that these two important signaling pathways are required for normal ENCDC migration and enteric ganglion formation in the developing hindgut ENS.

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Enteric glial cell diversification is influenced by spatiotemporal factors and source of neural progenitors in mice

Lefèvre,

Godefroid,

Soret

et al. 2024

Front. Neurosci.

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Previously focused primarily on enteric neurons, studies of the enteric nervous system (ENS) in both health and disease are now broadening to recognize the equally significant role played by enteric glial cells (EGCs). Commensurate to the vast array of gastrointestinal functions they influence, EGCs exhibit considerable diversity in terms of location, morphology, molecular profiles, and functional attributes. However, the mechanisms underlying this diversification of EGCs remain largely unexplored. To begin unraveling the mechanistic complexities of EGC diversity, the current study aimed to examine its spatiotemporal aspects in greater detail, and to assess whether the various sources of enteric neural progenitors contribute differentially to this diversity. Based on established topo-morphological criteria for categorizing EGCs into four main subtypes, our detailed immunofluorescence analyses first revealed that these subtypes emerge sequentially during early postnatal development, in a coordinated manner with the structural changes that occur in the ENS. When combined with genetic cell lineage tracing experiments, our analyses then uncovered a strongly biased contribution by Schwann cell-derived enteric neural progenitors to particular topo-morphological subtypes of EGCs. Taken together, these findings provide a robust foundation for further investigations into the molecular and cellular mechanisms governing EGC diversity.

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Harnessing the Power of Enteric Glial Cells’ Plasticity and Multipotency for Advancing Regenerative Medicine

Cited by 3 publications

References 151 publications

Glycyrol Relieves Ulcerative Colitis by Promoting the Fusion of ZO-1 with the Cell Membrane through the Enteric Glial Cells GDNF/RET Pathway

Glycyrol Relieves Ulcerative Colitis by Promoting the Fusion of ZO-1 with the Cell Membrane through the Enteric Glial Cells GDNF/RET Pathway

Essential Role of BMP4 Signaling in the Avian Ceca in Colorectal Enteric Nervous System Development

Enteric glial cell diversification is influenced by spatiotemporal factors and source of neural progenitors in mice

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