Has_circ_0000069 expression in breast cancer and its influences on prognosis and cellular activities

Abstract

Circular RNA (circRNA), as a newly discovered non-coding RNA with important regulatory potential, is closely related to the occurrence and progression of various tumors. This study aimed to investigate has_circ_0000069 expression in breast cancer and its influence on cellular activities. Using real-time quantitative polymerase chain reaction, has_circ_0000069 levels were measured in 137 pairs of tissue specimens, as well as cancer cell lines. The cellular activities of cell lines were determined by cell counting kit-8 (CCK-8) and Transwell assays. The potential targeting miRNAs were predicted and verified using an online database and dual-luciferase reporter assay. Has_circ_0000069 was highly expressed in breast cancer tissues and cells. The expression of has_circ_0000069 was associated with the five-year overall survival of patients. After silencing has_circ_0000069 in breast cancer cells, its expression reduced, and the ability of cell proliferation, migration, and invasion decreased. MiR-432 was verified as a targeting miRNA of has_circ_0000069. Has_circ_0000069 expression increased in breast cancer and was negatively related to patient's prognosis. Has_circ_0000069 may facilitate breast cancer tumor progression by sponging miR-432. These findings revealed that has_circ_0000069 may be a biomarker for predicting prognosis and a therapeutic target for treating patients with breast cancer.

GRIK1 promotes glioblastoma malignancy and is a novel prognostic factor of poor prognosis

“…The GRIK1 gene is known to be particularly implicated in schizophrenia within the glutamate system [ 21 ]. In addition, several recent reports have suggested that GRIK1 may be an important factor for predicting the progression or prognosis of colorectal carcinoma [ 22 ], and breast tumor [ 23 ], it is the most prevalent kinds of cancer in women and one of the main causes of cancer deaths [ 24 ], and the progression of breast cancer is also related to many elements, such as metastasis-related gene signature [ 25 ], and has_circ_0000069 [ 26 ]. A polymorphism (rs2832407) in GRIK1, which encodes the GluK1 kainate subunit, modifies topiramate-related reduction in heavy drinking [ 27 , 28 ], while a GRIKI polymorphism forecasts negative effects after topiramate therapy [ 28 ].…”