Hatching and harvesting techniques for Artemia cysts with different effects of salinity in the district of Situbondo, East Java

Bahr, Amelia; Isroni, Wahyu; Maulida, N.

doi:10.1088/1755-1315/718/1/012037

Cited by 12 publications

(12 citation statements)

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“…In accordance with previous studies, extreme salinities (low or high) and low temperatures are observed to inhibit Artemia hatching verifying the existence of optimal hatching conditions (Ahmed et al, 1997; Bahr et al, 2021; Hasan and Rabbane, 2018; Kumar and Babu, 2015; Sharahi and Zarei, 2016; Wasonga and Olendi, 2018). However, while these previous studies primarily focus on endpoint measurements of the hatching rate, here we have established relationships between the hatching rate and the progression of hatching and respiration behavior throughout the process.…”

Section: Discussionsupporting

confidence: 90%

“…Ahmed et al reported that the optimal salinity for hatchability is 20 ppt (Ahmed et al, 1997). In another study done by Bahr et al on the influence of salinity on hatching, the optimal hatchability was found at salinities of 60 ppt and temperatures around 30°C (Bahr et al, 2021). The variation in optimal parameters could be due to differences in test settings (e.g., light exposure (Arun et al, 2017; Asil et al, 2023; Sharahi and Zarei, 2016)) or variance in environmental conditions (container size, temperature/salinity gradients in bulk media).…”

Section: Introductionmentioning

confidence: 99%

“…The metabolism of the cysts can be resumed -and hatching initiated - when the environmental conditions are favorable (Liu et al, 2009), where the primary abiotic environmental parameters affecting hatchability are water temperature and salinity (Arun et al, 2017; Browne and Wanigasekera, 2000; Lenz and Browne, 2018). A number of previous studies have investigated the impact of these abiotic environmental parameters on the Artemia hatching performance (Ahmed et al, 1997; Bahr et al, 2021; Hasan and Rabbane, 2018; Kumar and Babu, 2015; Sharahi and Zarei, 2016; Wasonga and Olendi, 2018) which is evaluated through the measurement of the hatching rate at the endpoint of the experiment. For instance, Kumar et al observed that optimal hatching performance of Artemia occurred at 29°C and 29 parts per thousand (ppt) salinity (Kumar and Babu, 2015) while Sharahi et al found the optimal conditions to be 27-28°C and 35 ppt, respectively (Sharahi and Zarei, 2016).…”

Section: Introductionmentioning

confidence: 99%

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The impact of selected abiotic factors on zooplankton hatching process through real-time, in-situ observation

Dey¹,

Bradley

Boymelgreen³

2023

Preprint

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Current studies on abiotic impacts on marine microorganisms often focus on endpoint analysis (e.g., hatching rates, survival). Here, we demonstrate that a mechanistic understanding can be obtained through real-time measurement of respiration and morphology in controlled microenvironments over extended time periods. As a demonstration, temperature and salinity are chosen to represent critical abiotic parameters that are also threatened by climate change and a target species ofArtemia, a prominent zooplankton whose reproduction can affect the marine food pyramid. Different temperatures (20, 35, and 30°C) and salinities (0, 25, 50, and 75 ppt) are shown to significantly alter the duration of hatching stages, metabolic rates, and hatchability. Higher temperatures and moderate salinity boosted metabolic reactivation of latent cysts, while higher temperatures alone sped up the process. Hatchability is inversely related to the duration of the differentiation stage of hatching, which persisted longer at lower temperatures and salinities. Initial oxygen availability affects respiration but not hatchability owing to temperature and salinity interactions.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The impact of selected abiotic factors on zooplankton hatching process through real-time, in-situ observation

Dey¹,

Bradley

Boymelgreen³

2023

Preprint

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“…Ahmed et al reported that the optimal salinity for hatchability is 20 ppt 31 . In another study done by Bahr et al on the influence of salinity on hatching, the optimal hatchability was found at salinities of 60 ppt and temperatures around 30 °C 28 . The variation in optimal parameters could be due to differences in test settings (e.g., light exposure 25 , 30 , 32 ) or variance in environmental conditions (container size, temperature/salinity gradients in bulk media).…”

Section: Introductionmentioning

confidence: 89%

The impact of selected abiotic factors on Artemia hatching process through real-time observation of oxygen changes in a microfluidic platform

Dey

Bradley

Boymelgreen

2023

Sci Rep

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Current studies on abiotic impacts on Artemia, a crustacean which is widely used in aquaculture, and ecotoxicology, often focus on endpoint analysis (e.g., hatching rates, survival). Here, we demonstrate that a mechanistic understanding can be obtained through measurement of oxygen consumption in real-time over an extended time period in a microfluidic platform. The platform enables high level control of the microenvironment and direct observation of morphological changes. As a demonstration, temperature and salinity are chosen to represent critical abiotic parameters that are also threatened by climate change. The hatching process of Artemia consists of four different stages: hydration, differentiation, emergence, and hatching. Different temperatures (20, 35, and 30 °C) and salinities (0, 25, 50, and 75 ppt) are shown to significantly alter the duration of hatching stages, metabolic rates, and hatchability. Specifically, the metabolic resumption of dormant Artemia cysts was significantly enhanced at higher temperatures and moderate salinity, however, the time needed for this resumption was only dependent on higher temperatures. Hatchability was inversely related to the duration of the differentiation stage of hatching, which persisted longer at lower temperatures and salinities. The current approach of investigation of metabolism and corresponding physical changes can be employed to study hatching processes of other aquatic species, even those with low metabolic rate.

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“…al., 2017; Orire & Nwafor, 2008;Bahr et al, 2021). When testing with a wide range in hatching success.…”

mentioning

confidence: 99%

Silver nanoparticle toxicity on Artemia parthenogenetica nauplii hatched on axenic tryptic soy agar solid medium

Anh

Pham

Tran

et al. 2022

Preprint

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The use of gnobiotic brine shrimp (Artemia spp.) for ecotoxicology and bacteria-host interaction studies is common. However, requirements for axenic culture and matrix effects of seawater media can be an obstacle. Thus, we investigated the hatching ability of Artemia cysts on a novel sterile Tryptic Soy Agar (TSA) medium. We further optimized the culture conditions for temperature and salinity and assessed this culture system for toxicity screening of silver nanoparticles (AgNPs) across multiple biological endpoints. Maximal hatching of embryos occurred at 28°C and without addition of sodium chloride. When capsulated cysts were cultured on TSA solid medium Artemia were negatively impacted by AgNPs at 30–50 mgL-1 in terms of embryo hatching rate, umbrella- to nauplii-stage transformation rate, and nauplii-stage growth. At 50–100 mgL-1 AgNPs and higher, evidence of damage to lysosomal storage was recorded. At 500 mgL-1 AgNPs, development of the eye was inhibited and locomotory behavior impeded. Our study reveals that Artemia cysts can hatch on TSA solid medium which offers more controllability of axenic requirements.

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Hatching and harvesting techniques for Artemia cysts with different effects of salinity in the district of Situbondo, East Java

Cited by 12 publications

References 1 publication

The impact of selected abiotic factors on zooplankton hatching process through real-time, in-situ observation

The impact of selected abiotic factors on zooplankton hatching process through real-time, in-situ observation

The impact of selected abiotic factors on Artemia hatching process through real-time observation of oxygen changes in a microfluidic platform

Silver nanoparticle toxicity on Artemia parthenogenetica nauplii hatched on axenic tryptic soy agar solid medium

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