Hatching, survival and deformities of piracanjuba (Brycon orbignyanus) embryos subjected to different cooling protocols

Paes, Maria do Carmo Faria; Silva, Regiane Cristina da; Nascimento, Nivaldo Ferreira do; Valentin, Fernanda Nogueira; Senhorini, José Augusto; Nakaghi, Laura Satiko Okada

doi:10.1016/j.cryobiol.2014.10.003

Cited by 9 publications

(11 citation statements)

References 15 publications

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“…This fact is common to several teleost species while asynchrony occurs even within the same spawning (Nakatani et al, 2001). Currently, the cooling technique has had the best results (Ahammad et al, 2003), but damage/malformation is still common in post-cooling embryos (Neves et al, 2014;Digmayer, 2010;Paes et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Currently, the cooling technique has had the best results (Ahammad et al , 2003), but damage/malformation is still common in post-cooling embryos (Neves et al , 2014; Digmayer, 2010; Paes et al , 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Hatching and survival rates normally are the only indicators used to evaluate the success of the cooling technique of fish embryos (Desai et al , 2011). Furthermore, it is known that low temperatures affect larvae somehow, but there are few studies showing data on possible anomalies or proving that cryopreserved larvae can develop up to the sexual maturation phase (Paes et al , 2014).…”

Section: Introductionmentioning

confidence: 99%

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Post-cooling survival, growth and deformity rates in zebrafish embryos (Danio rerio)

Paes

Nakaghi

2017

Zygote

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This study investigated and analysed survival, growth and macro- and microscopic damage during the development of zebrafish embryos up to the adult stage after undergoing cooling. The embryos at 50% epiboly stage were selected, submerged in cryoprotectant solution of methanol and sucrose, cooled gradually to 0 ± 2°C temperature, and divided into two groups with different storage times (6 and 18 h). Subsequently, the embryos were reheated, rehydrated and incubated normally. The experiment lasted 5 months and, from hatching onward, the larvae were examined, collected and processed at pre-established time intervals. The hatching rate was significantly higher for the larvae stored for 18 h compared with the 6-h group. However, embryos from this group gave rise to a larger number of malformations, and these were much more severe compared with those in the 6 h group, which led to a higher mortality in the long term. Regarding larval length, the animals of the 6 h group had higher mean total length compared with the 18 h group, but both treatments were inferior to the control. Numerous macro- and microscopic malformations were observed and, in both treatments, only the morphologically normal individuals were able to develop to the adult stage, with organ development similar to the control, except for the gonads that were still undifferentiated in treated animals.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Post-cooling survival, growth and deformity rates in zebrafish embryos (Danio rerio)

Paes

Nakaghi

2017

Zygote

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“…Hubálek (2003) considered a concentration of 10% methanol as an ideal composition for the conservation solution of microorganisms. Similarly, several studies used concentrations of 9-10% methanol for fish embryos of species such as Rhinelepis aspera (Fornari et al, 2008), Colossoma macropomum (Pessoa et al, 2013), Brycon obignyanus (Fornari et al, 2014;Paes et al, 2014), Piaractus mesopotamicus (Lopes et al, 2014), and Piaractus brachypomus (Pessoa et al, 2015). In this study, we obtained an 80.85% hatching rate with 10% methanol for 6 h of cooling, and Pessoa et al (2013) observed an 88% hatching rate of Colossoma macropomum embryos treated with a 10% methanol solution.…”

Section: Methanol Concentration Dmso Concentration B Amentioning

confidence: 99%

“…Cooling and embryo freezing techniques, as well as cryoprotectant toxicity tests, have been studied in several species of native fish, in both species of economic interest and those endangered; some of the studied species are Piaractus mesopotamicus (Lopes et al, 2014), Piaractus brachypomus (Pessoa et al, 2015), Brycon orbignyanus (Fornari et al, 2014;Paes et al, 2014), and Prochilodus lineatus (Paula et al, 2014;Costa et al, 2017). Studies with ornamental fish species, such as Danio rerio, have also been conducted (Henn and Braunbeck, 2011;Kais et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol

et al. 2019

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This study aimed to evaluate the effect of using different concentrations (5, 7.5, 10, and 12.5%) of two permeating cryoprotectants, dimethyl sulphoxide (DMSO) and methanol, on Prochilodus lineatus embryos while being subjected to cooling for 2, 4, 6, and 8 h at 4 °C. We analyzed the hatching rate, viable larvae, and counts of hatched and spoiled eggs and those that did not complete their development after cooling. We observed a significant interaction among variables, permeable cryoprotectant concentration, and cooling time, having the increase of these factors caused a reduction in hatching rate. The curimba embryos showed a higher sensitivity to cold at a temperature of 4 °C for 6 and 8 h, directly influencing the production of viable larvae. We did not observe a significant difference in the variables analyzed when comparing the cryoprotectant solutions containing DMSO, methanol, and solutions containing only water and sucrose (0.5M). An increase in the concentration of cryoprotectant and cooling time promotes a reduction in the hatching rate and in the percentage of viable curimba larvae.

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Successful cryopreservation of common carp (Cyprinus carpio) embryos: efficiency of methanol-based vitrification solutions with honey on post-thaw survival rate

Keivanloo,

Sudagar,

Abualreesh

2023

Aquacult Int

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Hatching, survival and deformities of piracanjuba (Brycon orbignyanus) embryos subjected to different cooling protocols

Cited by 9 publications

References 15 publications

Post-cooling survival, growth and deformity rates in zebrafish embryos (Danio rerio)

Post-cooling survival, growth and deformity rates in zebrafish embryos (Danio rerio)

Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol

Successful cryopreservation of common carp (Cyprinus carpio) embryos: efficiency of methanol-based vitrification solutions with honey on post-thaw survival rate

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