Hawthorne, Margaret Fuller, and “Nemesis”

Warren, Austin

doi:10.2307/458585

Cited by 1 publication

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“…The T cell hybrid NIMP-TH1 was grown and stimulated with phorbol myristate acetate as described previously [5]. RNA was extracted [12] and the poly(A)-rich fraction purified by oligo(dT)-cellulose chromatography.…”

Section: Messenger R N a Preparation And Translationmentioning

confidence: 95%

“…EDA was assayed using femoral bone marrow cells from mice infected with Mesocestoides corti and differential counts were performed as described previously [5,271. The bone marrow was harvested 3 weeks after infection.…”

Section: Eosinophil Dflerentiation Activity ( E D a )mentioning

confidence: 99%

“…The T cell hybrid NIMP-TH1 [5] produces EDF after stimulation with phorbol myristate acetate but does not produce GM-CSF or IL-3, two more broadly acting regulators which also have activity in the EDF assay. mRNA was prepared from stimulated NIMP-TH1 cells and injected into Xenopus laevis oocytes.…”

Section: Isolation Of Cdna Clones Encoding Edfmentioning

confidence: 99%

mentioning

confidence: 95%

“…A number of observations have indicated the central role of T cells in parasiteinduced eosinophilia and have suggested the involvement of soluble mediators [l -41. More recently, Sanderson and colleagues have characterized a factor called eosinophil differentiation factor (EDF) produced by alloreactive T cell clones and by the T cell hybrid NIMP-TH1 [5]. Partially purified mouse EDF was shown specifically to stimulate the production of eosinophils in bone-marrow cultures and to have no detectable effect on the production of other myeloid cellsThe apparent specificity of EDF for the eosinophil lineage in myeloid haemopoiesis contrasts with the properties of other known regulators [7] affecting granulocyte formation such as granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3) or multi-colony-stimulating factor (multi-CSF), and granulocyte-colony-stimulating factor (G-CSF) and suggests that EDF may play a central role in the PI.…”

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Isolation, structure and expression of cDNA and genomic clones for murine eosinophil differentiation factor

Campbell

Sanderson

Wang

et al. 1988

European Journal of Biochemistry

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Eosinophil differentiation factor (EDF) is a recently described regulator affecting eosinophil growth and activation. cDNA clones for murine EDF were isolated by direct expression from libraries prepared from the T cell hybrid NIMP-TH1. The longest cDNA clone obtained was 1534 bp in length encoding a polypeptide of 133 amino acids. Two variant cDNAs suggesting alternative RNA processing events were detected. The EDF gene was cloned from a genomic /z library and a region of 6727 bp encompassing the gene was sequenced. The gene contains three introns 829, 1875 and 79 bases in length and has numerous repetitive sequences. A common, possible regulatory element, including a conserved decamer, lies adjacent to the TATA boxes of the EDF and granulocyte-macrophage colony-stimulating factor (GM-CSF) genes and similar sequences are present in some other lymphokine genes. Recombinant EDF produced in monkey COS cells strongly stimulated the eosinophil lineage and also showed B-cell-growth factor I1 (BCGFII) activity whereas recombinant murine interleukin-3 and GM-CSF showed much broader activity towards the different myeloid lineages, were less active on eosinophils and had no BCGFII activity. The BCGFII activity of recombinant EDF together with a comparison of the BCGFII (interleukin-5) cDNA sequence with that of the EDF cDNA establishes that these two activities are the properties of a single polypeptide.Eosinophilia is a characteristic part of the mammalian host response to metazoan parasites. A number of observations have indicated the central role of T cells in parasiteinduced eosinophilia and have suggested the involvement of soluble mediators [l -41. More recently, Sanderson and colleagues have characterized a factor called eosinophil differentiation factor (EDF) produced by alloreactive T cell clones and by the T cell hybrid NIMP-TH1 [5]. Partially purified mouse EDF was shown specifically to stimulate the production of eosinophils in bone-marrow cultures and to have no detectable effect on the production of other myeloid cellsThe apparent specificity of EDF for the eosinophil lineage in myeloid haemopoiesis contrasts with the properties of other known regulators [7] affecting granulocyte formation such as granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3) or multi-colony-stimulating factor (multi-CSF), and granulocyte-colony-stimulating factor (G-CSF) and suggests that EDF may play a central role in the PI.Correspondence to H. D. Campbell, Medical Molecular Biology Group, John Curtin School of Medical Research, The Australian National University, GPO Box 334, Canberra, Australian Capital Territory, Australia 2601Abbreviations. IL-5, interleukin-5; IL-4, interleukin-4; IL-3, interleukin-3; EDF, eosinophil differentiation factor; TRF, T-cellreplacing factor; BCGFII, B-cell-growth factor 11; GM-CSF, granulocyte-macrophage colony-stimulating factor; G-CSF, granulocyte colony-stimulating factor; CSF, colony-stimulating factor; r, recombinant; EDA, eosinophil differentiation activity. regulat...

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Section: Messenger R N a Preparation And Translationmentioning

confidence: 95%

Section: Eosinophil Dflerentiation Activity ( E D a )mentioning

confidence: 99%

Section: Isolation Of Cdna Clones Encoding Edfmentioning

confidence: 99%

mentioning

confidence: 95%

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confidence: 99%

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