Amino acids are major sources of nitrogen in plants and soil. Amino acids areone of the most important parameter for growth of plants and health of soil. In the present study, fungicide Zole (tebuconazole 18.3% + Azoxytrobin 11%) has been added in soil and its effect on soil has been studied in presence of selected amino acids. All the selected amino acids are non-polar and different techniques used in current study include soil thin layer chromatography, X-ray diffraction (XRD) and scanning electron microscopy (SEM). It has been found that the mobility of amino acid decrease with the increase in concentration of fungicidezole in the soil. Theresults also confirmed the improvement of soil quality in terms of amino acid adsorption in presence of fungicide Zole.