Hazelnut protein-derived peptide LDAPGHR shows anti-inflammatory activity on LPS-induced RAW264.7 macrophage

Ren, Dayong; Wang, Peng; Liu, Chunlei; Wang, Ji; Liu, Xiuqi; Liu, Jingsheng; Min, Weihong

doi:10.1016/j.jff.2018.04.024

Cited by 42 publications

(23 citation statements)

References 33 publications

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“…In the present study, the 500 and 1000 μg/mL of LPF treatment groups significantly reduced the mRNA expressions of IκB-α in LPS-induced RAW264.7 cells (Figure 4E). Similar results were reported in other food-derived peptides (Ren et al, 2018;Yi et al, 2020). Cyclooxygenase enzymes-2 (COX-2) is an essential rate-limiting enzyme involved in catalyzing the biosynthesis of pro-inflammatory factor prostaglandin E2 (PGE2) from arachidonic acid (Xue et al, 2020).…”

Section: Discussionsupporting

confidence: 74%

Potential anti-inflammatory activity of walnut protein derived peptide leucine-proline-phenylalanine in lipopolysaccharides-irritated RAW264.7 cells

Wang

Zhi

Han

et al. 2021

Food and Agricultural Immunology

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The purpose of this study was to explore the anti-inflammatory mechanism of walnut peptide LPF in the LPS-induced RAW264.7 cells model. The walnut peptide LPF down-regulated the phagocytic ability and the production of nitric oxide (NO) induced by LPS-stimulated RAW264.7. Molecular docking analysis showed that the walnut peptide LPF can bind into the active pocket of inducible nitric oxide synthase (iNOS) with high affinity. Further, the mRNA expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and tumour necrosis factor-α (TNF-α) were suppressed by the walnut peptide LPF intervention. Later, we validated the under-expression of INOS protein by western blot. The decreased mRNA expression of IκB-α demonstrated the anti-inflammatory effects of the walnut peptide LPF were related to the regulation of the NF-κB signalling pathway. Our results suggest that the walnut peptide LPF has the potential to be developed as a functional food ingredient against inflammation.

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Section: Discussionsupporting

confidence: 74%

Potential anti-inflammatory activity of walnut protein derived peptide leucine-proline-phenylalanine in lipopolysaccharides-irritated RAW264.7 cells

Wang

Zhi

Han

et al. 2021

Food and Agricultural Immunology

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“…Macrophages are monocyte-derived phagocytic cells, which play an important role in innate immune defense and acquired immune responses. Therefore, the proliferative activity of macrophages can be used as an indicator of the involvement of immune activators in cytotoxicity [41,42]. The results of the MTT assay demonstrated that NJP treatment had a significant effect on RAW264.7 macrophage viability at the indicated concentrations of 12.5~1000 μg/mL (Figure 3B).…”

Section: Resultsmentioning

confidence: 99%

Immunomodulatory Activity of Low Molecular-Weight Peptides from Nibea japonica in RAW264.7 Cells via NF-κB Pathway

Zhang

Lin

et al. 2019

Marine Drugs

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In this study, a low molecular-weight (Mw) peptide named NJP (<1 kDa), was purified from a protein hydrolysate of Nibea japonica by ultrafiltration, and its immunomodulatory effect on RAW264.7 cells was evaluated. The lactate dehydrogenase (LDH) and MTT assays were performed to explore the cytotoxicity of NJP. The results showed that NJP promoted cell proliferation and had no significant toxic effects on RAW264.7 cells. Moreover, the cells formed multiple pseudopodia indicating that they were in activated state. Further tests showed that NJP significantly promoted phagocytic capacity, and the secretion of proinflammatory cytokines tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β). It also increased the synthesis of nitric oxide (NO) by upregulating inducible nitric oxide synthase (iNOS) protein level. Flow cytometry revealed that NJP promoted cell cycle progression and increased the percentage of cells in G0/G1 phase. NJP promoted IκBα degradation, p65 and nuclear factor (NF)-κB activation and translocation by up-regulating IKKα/β protein expression. In conclusion, these results indicated that NJP exerts immunomodulatory effects on RAW264.7 cells through the NF-κB signaling pathway. Therefore, NJP can be incorporated in the production of functional foods or nutraceuticals.

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“…22 The mentioned results are consistent with recent reports that the treatment with peptides can inhibit NO synthesis in LPS-induced RAW264.7 cells. 23 Fig. 1 The cell cytotoxicity of millet bran peptides on the LPS-induced RAW 264.7 cells.…”

Section: Millet Bran Peptide Treatment Inhibited the Production Of Pr...mentioning

confidence: 99%

Anti-inflammatory activity of peptides derived from millet bran in vitro and in vivo

Liu

Zou

et al. 2022

Food Funct.

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Hazelnut protein-derived peptide LDAPGHR shows anti-inflammatory activity on LPS-induced RAW264.7 macrophage

Cited by 42 publications

References 33 publications

Potential anti-inflammatory activity of walnut protein derived peptide leucine-proline-phenylalanine in lipopolysaccharides-irritated RAW264.7 cells

Potential anti-inflammatory activity of walnut protein derived peptide leucine-proline-phenylalanine in lipopolysaccharides-irritated RAW264.7 cells

Immunomodulatory Activity of Low Molecular-Weight Peptides from Nibea japonica in RAW264.7 Cells via NF-κB Pathway

Anti-inflammatory activity of peptides derived from millet bran in vitro and in vivo

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