HcSTK, a Caenorhabditis elegans PAR-1 homologue from the parasitic nematode, Haemonchus contortus

Nikolaou, Sia; Hartman, Dadna; Presidente, P. J. A.; Newton, Susan E; Gasser, Robin B.

doi:10.1016/s0020-7519(02)00008-5

Cited by 35 publications

(39 citation statements)

References 41 publications

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“…Recently, we isolated and characterized multiple, alternatively spliced cDNAs encoding a PAR-1 homologue (designated HcSTK) from the socio-economically important parasitic nematode Haemonchus contortus (Strongylida: Trichostrongylidae), and qualitatively examined mRNA expression in different developmental stages and organs of the parasite (Nikolaou et al, 2002). The amino acid sequence similarity among members of the PAR-1/ MARK STK subfamily, representing a range of species of vertebrates, invertebrates (including C. elegans) and yeast, suggested that HcSTK may be involved in a conserved eukaryotic signal transduction pathway.…”

Section: Introductionmentioning

confidence: 99%

“…The amino acid sequence similarity among members of the PAR-1/ MARK STK subfamily, representing a range of species of vertebrates, invertebrates (including C. elegans) and yeast, suggested that HcSTK may be involved in a conserved eukaryotic signal transduction pathway. Specifically, there was 93% sequence similarity in the catalytic domain between HcSTK and PAR-1 of C. elegans (see Nikolaou et al, 2002). While the proposal for a conserved role of the latter two molecules is also supported by a relatively close genetic relationship of H. contortus and C. elegans (see Blaxter et al, 1998;Blaxter, 1998), there are significant biological differences between the parasitic nematode (in that it is dioecious and undergoes pre-parasitic and parasitic phases of development) and the free-living one (which is hermaphroditic/male and exclusively free-living).…”

Section: Introductionmentioning

confidence: 99%

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Genomic organization and expression analysis for hcstk, a serine/threonine protein kinase gene of Haemonchus contortus, and comparison with Caenorhabditis elegans par-1

Nikolaou

Hartman

Nisbet

et al. 2004

Gene

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genomic organization and expression analysis for hcstk, a serine/threonine protein kinase gene of Haemonchus contortus, and comparison with Caenorhabditis elegans par-1

Nikolaou

Hartman

Nisbet

et al. 2004

Gene

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“…In brief, H. contortus was produced in helminth-free lambs, and larval stages were produced as described by Nikolaou et al (2002). Faecal cultures were collected, and first-stage larvae (L1) were recovered after 1 day and infective thirdstage larvae (L3) after 6-7 days incubation at 27°C.…”

Section: Nematodes and Culture Conditionsmentioning

confidence: 99%

A rapid colorimetric assay for the quantitation of the viability of free-living larvae of nematodes in vitro

James

Davey

2007

Parasitol Res

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With increasing drug resistance in gastrointestinal parasites, identification of new anthelmintics is essential. The non-parasitic nematode Caenorhabditis elegans is used extensively as a model to identify drug targets and potential novel anthelmintics because it can be readily cultured in vitro. Traditionally, the assessment of worm viability has relied on labour-intensive developmental and behavioral assays. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide-formazan (MTT-formazan) colorimetric assay uses metabolic activity as a marker of viability in mammalian cell culture systems and has been applied for use with filarial nematodes. In the present study, this assay has been optimized and validated to rapidly assess the viability of C. elegans after drug treatment. Living, but not dead, C. elegans take up MTT and reduce it to the blue formazan, providing visual, qualitative, and quantitative assessment of viability. MTT at a concentration of 5 mg/ml with 3 h incubation was optimal for detecting changes in viability with drug treatment. We have applied this assay to quantitate the effects of ivermectin and short-chain alcohols on the viability of C. elegans. This assay is also applicable to first-stage larvae of the parasitic nematode Haemonchus contortus. The advantage of this assay is the rapid quantitation in screening drugs to identify potential anthelmintics.

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“…Nikolaou et al 2002) using cDNA probes derived from a C. elegans gene (accession no. U49263) and employing a standard approach (Sambrook et al 1989).…”

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confidence: 99%

“…Guo and Kemphues, 1996), whether other myosin isoforms play a role and whether the interaction with non-muscle myosins is specific. Addressing such questions may elucidate whether interactions exist between the myosins isolated herein and a serine/threonine kinase (HcSTK) (Nikolaou et al 2002(Nikolaou et al , 2004, a PAR-1 homologue from H. contortus.…”

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confidence: 99%

Isolation and characterization of class II myosin genes from Haemonchus contortus

Nikolaou

Chilton

et al. 2006

Parasitol Res

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In this study, cDNAs encoding myosin from the parasitic nematode Haemonchus contortus were isolated and characterized. Several exhibited a considerable degree of sequence variation at the nucleotide and limited divergence at the amino acid levels within the various functional domains. The results suggest that the cDNAs isolated represented a single myosin heavy chain, which, by comparison with a number of other myosins, is inferred to represent a homologue of a muscle myosin (CeMHCA) of the free-living nematode Caenorhabditis elegans. The findings could have implications for investigating cytoskeletal dynamics and/or signalling pathways.

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HcSTK, a Caenorhabditis elegans PAR-1 homologue from the parasitic nematode, Haemonchus contortus

Cited by 35 publications

References 41 publications

Genomic organization and expression analysis for hcstk, a serine/threonine protein kinase gene of Haemonchus contortus, and comparison with Caenorhabditis elegans par-1

Genomic organization and expression analysis for hcstk, a serine/threonine protein kinase gene of Haemonchus contortus, and comparison with Caenorhabditis elegans par-1

A rapid colorimetric assay for the quantitation of the viability of free-living larvae of nematodes in vitro

Isolation and characterization of class II myosin genes from Haemonchus contortus

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