HcToll3 was involved in anti- Vibrio defense in freshwater pearl mussel, Hyriopsis cumingii

Zhang, Hongwei; Huang, Ying; Man, Xin; Wang, Yue; Hui, Kai-Min; Yin, Shaowu; Zhang, Xiaowen

doi:10.1016/j.fsi.2017.02.015

Cited by 19 publications

(7 citation statements)

References 35 publications

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“…Many Toll genes were obtained from a de novo transcriptomic library of H. cumingii . In our previous study, we cloned and characterized five HcToll receptors, including HcToll1, HcToll-I, HcToll2-1, HcToll2-2 , and HcToll3 (Ren et al, 2013 , 2014 ; Cao et al, 2016 ; Zhang et al, 2017 ). These HcTolls participate in H. cumingii innate immunity.…”

Section: Discussionmentioning

confidence: 99%

“…LRR_CT domain free Toll was rarely reported. In H. cumingii , HcToll3 also has no LRR_CT domain (Zhang et al, 2017 ). HcToll1 and HcToll2 both have two LRR_CT domains (Ren et al, 2013 , 2014 ).…”

Section: Discussionmentioning

confidence: 99%

“…HcToll1 and HcToll2 both have two LRR_CT domains (Ren et al, 2013 , 2014 ). Similar to HcToll3, HcToll4, and HcToll5 both have a LRR_NT domain located behind signal peptide (Zhang et al, 2017 ). From the phylogenetic tree, HcToll4 and HcToll5 showed closer relationship with HcToll3 than HcToll1, HcToll2s, and HcToll-I.…”

Section: Discussionmentioning

confidence: 99%

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Cloning and Analysis of Gene Expression of Two Toll Receptors in Freshwater Pearl Mussel Hyriopsis cumingii

2018

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Toll receptors are involved in innate immunity of invertebrates. In this study, we identify and characterize two Toll genes (named HcToll4 and HcToll5) from triangle sail mussel Hyriopsis cumingii. HcToll4 has complete cDNA sequence of 3,162 bp and encodes a protein of 909 amino acids. HcToll5 cDNA is 4,501 bp in length and encodes a protein of 924 amino acids. Both deduced HcToll4 and HcToll5 protein contain signal peptide, extracellular leucine rich repeats (LRRs), and intracellular Toll/interleukin-1 (IL-1) receptor domains. Quantitative real-time PCR analysis revealed that HcToll4 and HcToll5 were largely distributed in the hepatopancreas and could be detected in the gills and mantle. HcToll4 and HcToll5 expression could respond to Staphylococcus aureus, Vibrio parahaemolyticus, White spot syndrome virus (WSSV) or Poly I:C challenge. RNA interference by siRNA results showed that HcToll4 and HcToll5 were involved in the regulation of theromacin (HcThe) and whey acidic protein (HcWAP) expression. Based on these results, HcToll4 and HcToll5 might play pivotal function in H. cumingii innate immune response.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Cloning and Analysis of Gene Expression of Two Toll Receptors in Freshwater Pearl Mussel Hyriopsis cumingii

2018

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“…Astonishingly, Cg TLR1–4 from C. gigas lack the activities to recognize specific PAMPs, but they can activate NF-κB responsive reporter in HEK293 cells with a dose-dependent manner [ 49 ], which supports the functional differentiation of these primordial TLRs in molluscs. Moreover, three TLRs ( Hc Toll1, Hc Toll-2, and Hc Toll3) from the pearl mussel H. cumingii could respond to bacterial and viral challenge and regulate the expression of antimicrobial peptides (AMPs) [ 50 , 51 , 52 ]. A rather canonical MyD88-dependent TLR pathway was also demonstrated in scallop and this pathway was involved in immune signaling to activate downstream anti-oxidant, anti-bacteria reaction, and apoptosis [ 53 ].…”

Section: Canonical Carbohydrates Recognition Protein In Molluscsmentioning

confidence: 99%

Pathogen-Derived Carbohydrate Recognition in Molluscs Immune Defense

Wang

Song

Wang

et al. 2018

IJMS

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Self-nonself discrimination is a common theme for all of the organisms in different evolutionary branches, which is also the most fundamental step for host immune protection. Plenty of pattern recognition receptors (PRRs) with great diversity have been identified from different organisms to recognize various pathogen-associated molecular patterns (PAMPs) in the last two decades, depicting a complicated scene of host-pathogen interaction. However, the detailed mechanism of the complicate PAMPs–PRRs interactions at the contacting interface between pathogens and hosts is still not well understood. All of the cells are coated by glycosylation complex and thick carbohydrates layer. The different polysaccharides in extracellular matrix of pathogen-host are important for nonself recognition of most organisms. Coincidentally, massive expansion of PRRs, majority of which contain recognition domains of Ig, leucine-rich repeat (LRR), C-type lectin (CTL), C1q and scavenger receptor (SR), have been annotated and identified in invertebrates by screening the available genomic sequence. The phylum Mollusca is one of the largest groups in the animal kingdom with abundant biodiversity providing plenty of solutions about pathogen recognition and immune protection, which might offer a suitable model to figure out the common rules of immune recognition mechanism. The present review summarizes the diverse PRRs and common elements of various PAMPs, especially focusing on the structural and functional characteristics of canonical carbohydrate recognition proteins and some novel proteins functioning in molluscan immune defense system, with the objective to provide new ideas about the immune recognition mechanisms.

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“…Understanding the native response against infectious diseases is crucial for the sustainable production of this species. In previous studies, seven Tolls, namely, HcToll1 (Ren et al, 2013), HcToll2-1 (Ren et al, 2014), HcTollI , HcToll2-2 (Cao et al, 2016), HcToll3 (Zhang et al, 2017), HcToll4 , and HcToll5 (Huang Y. et al, 2018), have been cloned and identified in H. cumingii , and they play crucial roles in bacterial and viral infections. However, the significance of mussel Tolls to immune responses at a protein level has been rarely discussed, and we have yet to fully elucidate the molecular mechanisms involved in how this pathway regulates immune responses.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Two Toll Receptors in Hyriopsis cumingii and Their Potential Roles in Antibacterial Response

2019

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Tolls/Toll-like receptors (TLRs) play a key role in innate immunity by detecting the invading microbes and subsequently activating downstream signaling cascades. In this study, two new molluscan Toll members (designed as HcToll6 and HcToll7 ) were identified from triangle-shell pearl mussel ( Hyriopsis cumingii ). The obtained HcToll6 full-length cDNA was 3207 bp consisting of a 2223 bp open reading frame (ORF) that encoded a peptide of 740 amino acids. HcToll7 cDNA is a 3216 bp molecule that contains an ORF of 2139 bp encoding a protein of 712 amino acids. The deduced HcToll6 and HcToll7 proteins share two common structures: extracellular leucine-rich repeat (LRR) domains and intracellular Toll/interleukin-1 receptor (TIR) domain. Quantitative real-time PCR results showed that HcToll6 and HcToll7 were mainly expressed in the hepatopancreas and the gills, and they responded rapidly to bacterial stimulation. RNA interference by dsRNA results revealed that HcToll6 and HcToll7 RNAi strongly decreased the expression of lysozyme ( HcLyso ) and defensin ( HcDef ) in the gills of RNAi-treated mussels with Vibrio parahaemolyticus challenge. As a pattern recognition receptor, the prokaryotic expressed the recombinant LRR domains of HcToll6 and HcToll7 (rHcToll6-LRR and rHcToll7-LRR) could bind to Gram-positive and Gram-negative bacteria and had a strong tendency to recognize lipopolysaccharide (LPS) and peptidoglycan (PNG). rHcToll6-LRR and rHcToll7-LRR exhibited a significant in vitro bactericidal activity against V. parahaemolyticus and Staphylococcus aureus . These findings provide useful information to characterize Tolls in mussels.

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HcToll3 was involved in anti- Vibrio defense in freshwater pearl mussel, Hyriopsis cumingii

Cited by 19 publications

References 35 publications

Cloning and Analysis of Gene Expression of Two Toll Receptors in Freshwater Pearl Mussel Hyriopsis cumingii

Cloning and Analysis of Gene Expression of Two Toll Receptors in Freshwater Pearl Mussel Hyriopsis cumingii

Pathogen-Derived Carbohydrate Recognition in Molluscs Immune Defense

Molecular Characterization of Two Toll Receptors in Hyriopsis cumingii and Their Potential Roles in Antibacterial Response

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