HD2A and HD2C co-regulate drought stress response by modulating stomatal closure and root growth in Arabidopsis

Tahir, Muhammad; Karagiannis, Jim; Tian, Lining

doi:10.3389/fpls.2022.1062722

Cited by 9 publications

(6 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The survey revealed that, in the stable QTL 1, B. napus gene BnaA07p10740 ( At1g29290 , C‐TERMINALLY ENCODED PEPTIDE 14 , CEP14 ) contributes to root biomass production (Huang et al, 2023b); BnaA07p10110 ( At1g29950 , SUPPRESSOR OF ACAULIS LIKE 3 , SACL3 ) to root meristem size and root growth (Katayama et al, 2015); BnaA07p10090 ( At1g30040 , GIBBERELLIN 2‐OXIDASE 2 , GA2OX2 ) to gibberellic acid homeostasis for maintaining root growth (Tahir et al, 2022); and BnaA07p10020 ( At1g30270, CBL‐INTERACTING PROTEIN KINASE 23, CIPK23 ) to environmental stress response, including nitrate imbalance (Ródenas & Vert, 2021). In the stable QTL 2, C07p00330 ( At2g20610 , SUPERROOT 1 , SUR1 ) regulates auxin biosynthesis and lateral root patterning (Boerjan et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

Genetic control of root morphology in response to nitrogen across rapeseed diversity

Haelterman,

Louvieaux,

Chiodi

et al. 2024

Physiologia Plantarum

View full text Add to dashboard Cite

Rapeseed (Brassica napus L.) is an oil‐containing crop of great economic value but with considerable nitrogen requirement. Breeding root systems that efficiently absorb nitrogen from the soil could be a driver to ensure genetic gains for more sustainable rapeseed production. The aim of this study is to identify genomic regions that regulate root morphology in response to nitrate availability. The natural variability offered by 300 inbred lines was screened at two experimental locations. Seedlings grew hydroponically with low or elevated nitrate levels. Fifteen traits related to biomass production and root morphology were measured. On average across the panel, a low nitrate level increased the root‐to‐shoot biomass ratio and the lateral root length. A large phenotypic variation was observed, along with important heritability values and genotypic effects, but low genotype‐by‐nitrogen interactions. Genome‐wide association study and bulk segregant analysis were used to identify loci regulating phenotypic traits. The first approach nominated 319 SNPs that were combined into 80 QTLs. Three QTLs identified on the A07 and C07 chromosomes were stable across nitrate levels and/or experimental locations. The second approach involved genotyping two groups of individuals from an experimental F2 population created by crossing two accessions with contrasting lateral root lengths. These individuals were found in the tails of the phenotypic distribution. Co‐localized QTLs found in both mapping approaches covered a chromosomal region on the A06 chromosome. The QTL regions contained some genes putatively involved in root organogenesis and represent selection targets for redesigning the root morphology of rapeseed.

show abstract

Section: Discussionmentioning

confidence: 99%

Genetic control of root morphology in response to nitrogen across rapeseed diversity

Haelterman,

Louvieaux,

Chiodi

et al. 2024

Physiologia Plantarum

View full text Add to dashboard Cite

show abstract

“…In Arabidopsis, loss of HDA19 could enhance plant drought resistance [ 79 ], whereas the drought tolerance was reduced in hda9 mutants [ 36 ]. The Arabidopsis HD2A, HD2C, and HD2D were described as positive regulators of drought resistance since overexpression of these three genes played enhanced tolerance to drought stress when compared with WT plants [ 47 , 48 , 50 ]. While in another study, the silencing of HD2D represses enap1enap2myb44 drought-sensitive phenotype, indicating HD2D negatively regulates plant drought response [ 80 ].…”

Section: Discussionmentioning

confidence: 99%

“…Based on our results, we propose a model for the regulatory function of HD2A and HD2B in coordinating drought stress response (Figure 10). Under drought conditions, the expression of HD2A and HD2B was enhanced [48] and functioned together to enhance the deacetylation of NCED9 and ABA-dependent/independent drought-responsive genes, thus repressing the endogenous ABA synthesis and drought responses. Additionally, drought stress increases the endogenous ABA content, which represses the expression of HD2A and HD2B, suggesting a negative feedback loop between ABA and HD2A/HD2B accumulation (Figure 9B).…”

Section: Hd2a and Hd2b Function In Drought Tolerance By Affecting Aba...mentioning

confidence: 99%

“…Expression of all four members of the plant-specific histone deacetylase was repressed by exogenous application of ABA and salt [ 46 ] suggesting a negatively regulatory role of HD2s in stress response. Surprisingly, overexpression of HD2A, HD2C, and HD2D enhanced tolerance to drought and salt stress in Arabidopsis [ 47 , 48 ]. Moreover, silencing of HD2C conferred an ABA-sensitive phenotype and decreased tolerance to salt stress and heat stress of the mutant line [ 46 , 49 , 50 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Histone Deacetylases HD2A and HD2B Undergo Feedback Regulation by ABA and Modulate Drought Tolerance via Mediating ABA-Induced Transcriptional Repression

Han,

Haouel,

Georgii

et al. 2023

Genes

View full text Add to dashboard Cite

Histone deacetylation catalyzed by histone deacetylase plays a critical role in gene silencing and subsequently controls many important biological processes. It was reported that the expression of the plant-specific histone deacetylase subfamily HD2s is repressed by ABA in Arabidopsis. However, little is known about the molecular relationship between HD2A/HD2B and ABA during the vegetative phase. Here, we describe that the hd2ahd2b mutant shows hypersensitivity to exogenous ABA during the germination and post-germination period. Additionally, transcriptome analyses revealed that the transcription of ABA-responsive genes was reprogrammed and the global H4K5ac level is specifically up-regulated in hd2ahd2b plants. ChIP-Seq and ChIP-qPCR results further verified that both HD2A and HD2B could directly and specifically bind to certain ABA-responsive genes. As a consequence, Arabidopsis hd2ahd2b plants displayed enhanced drought resistance in comparison to WT, which is consistent with increased ROS content, reduced stomatal aperture, and up-regulated drought-resistance-related genes. Moreover, HD2A and HD2B repressed ABA biosynthesis via the deacetylation of H4K5ac at NCED9. Taken together, our results indicate that HD2A and HD2B partly function through ABA signaling and act as negative regulators during the drought resistance response via the regulation of ABA biosynthesis and response genes.

show abstract

“…In Arabidopsis thaliana, a model plant, the HD2-type histone deacetylase HD2C has been found to negatively regulate drought tolerance. HD2C represses the expression of drought-responsive genes by deacetylating histones in their promoter regions, leading to reduced gene expression and impaired drought response in the plant [29]. Conversely, in Arabidopsis, certain genes positively regulate salt stress tolerance by enhancing the expression of salt-responsive genes.…”

Section: Acetylation and Deacetylationmentioning

confidence: 99%

Post-Translational Modifications in Histones and Their Role in Abiotic Stress Tolerance in Plants

Sharma,

Sidhu,

Samota

et al. 2023

Proteomes

View full text Add to dashboard Cite

Abiotic stresses profoundly alter plant growth and development, resulting in yield losses. Plants have evolved adaptive mechanisms to combat these challenges, triggering intricate molecular responses to maintain tissue hydration and temperature stability during stress. A pivotal player in this defense is histone modification, governing gene expression in response to diverse environmental cues. Post-translational modifications (PTMs) of histone tails, including acetylation, phosphorylation, methylation, ubiquitination, and sumoylation, regulate transcription, DNA processes, and stress-related traits. This review comprehensively explores the world of PTMs of histones in plants and their vital role in imparting various abiotic stress tolerance in plants. Techniques, like chromatin immune precipitation (ChIP), ChIP-qPCR, mass spectrometry, and Cleavage Under Targets and Tag mentation, have unveiled the dynamic histone modification landscape within plant cells. The significance of PTMs in enhancing the plants’ ability to cope with abiotic stresses has also been discussed. Recent advances in PTM research shed light on the molecular basis of stress tolerance in plants. Understanding the intricate proteome complexity due to various proteoforms/protein variants is a challenging task, but emerging single-cell resolution techniques may help to address such challenges. The review provides the future prospects aimed at harnessing the full potential of PTMs for improved plant responses under changing climate change.

show abstract

HD2A and HD2C co-regulate drought stress response by modulating stomatal closure and root growth in Arabidopsis

Cited by 9 publications

References 78 publications

Genetic control of root morphology in response to nitrogen across rapeseed diversity

Genetic control of root morphology in response to nitrogen across rapeseed diversity

Histone Deacetylases HD2A and HD2B Undergo Feedback Regulation by ABA and Modulate Drought Tolerance via Mediating ABA-Induced Transcriptional Repression

Post-Translational Modifications in Histones and Their Role in Abiotic Stress Tolerance in Plants

Contact Info

Product

Resources

About