HDL-C vs HDL-P: How Changing One Letter Could Make a Difference in Understanding the Role of High-Density Lipoprotein in Disease

Davidson, W. Sean

doi:10.1373/clinchem.2014.232769

Cited by 28 publications

(25 citation statements)

References 14 publications

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“…LDL, IDL, Recent studies have suggested that NMR measurements of LDL-P and HDL-P particle numbers (which we have referred to as the total concentration of LDL NMR and HDL NMR particles) may have advantages for the prediction of cardiovascular events over the traditional measurement of LDL-C or HDL-C (31). However, it is important to note that although NMR measurements seem to be strongly correlated with HDL number and size, the absolute accuracy of this approach for the true abundance of HDL particles in plasma has not been clearly established, and that lipoprotein particle size measured by NMR spectroscopy and other laboratory methods has produced different results (32).…”

Section: Discussionmentioning

confidence: 99%

Serum Apolipoprotein A-I and Large High-Density Lipoprotein Particles Are Positively Correlated with FEV₁ in Atopic Asthma

Barochia¹,

Kaler²,

Cuento³

et al. 2015

Am J Respir Crit Care Med

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Rationale: Although lipids, apolipoproteins, and lipoprotein particles are important modulators of inflammation, varying relationships exist between these parameters and asthma.Objectives: To determine whether serum lipids and apolipoproteins correlate with the severity of airflow obstruction in subjects with atopy and asthma.Methods: Serum samples were obtained from 154 atopic and nonatopic subjects without asthma, and 159 subjects with atopy and asthma. Serum lipid and lipoprotein levels were quantified using standard diagnostic assays and nuclear magnetic resonance (NMR) spectroscopy. Airflow obstruction was assessed by FEV 1 % predicted.Measurements and Main Results: Serum lipid levels correlated with FEV 1 only in the subjects with atopy and asthma. Serum levels of high-density lipoprotein (HDL) cholesterol and apolipoprotein A-I (apoA-I) were positively correlated with FEV 1 in subjects with atopy and asthma, whereas a negative correlation existed between FEV 1 and serum levels of triglycerides, low-density lipoprotein (LDL) cholesterol, apolipoprotein B (apoB), and the apoB/apoA-I ratio. NMR spectroscopy identified a positive correlation between FEV 1 and HDL NMR particle size, as well as the concentrations of large HDL NMR particles and total IDL NMR (intermediate-density lipoprotein) particles in subjects with atopy and asthma. In contrast, LDL NMR particle size and concentrations of LDL NMR and VLDL NMR (very-low-density lipoprotein) particles were negatively correlated with FEV 1 in subjects with atopy and asthma.Conclusions: In subjects with atopy and asthma, serum levels of apoA-I and large HDL NMR particles are positively correlated with FEV 1 , whereas serum triglycerides, LDL cholesterol, and apoB are associated with more severe airflow obstruction. These results may facilitate future studies to assess whether apoA-I and large HDL NMR particles can reduce airflow obstruction and disease severity in asthma.

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Section: Discussionmentioning

confidence: 99%

Serum Apolipoprotein A-I and Large High-Density Lipoprotein Particles Are Positively Correlated with FEV₁ in Atopic Asthma

Barochia¹,

Kaler²,

Cuento³

et al. 2015

Am J Respir Crit Care Med

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“…The primary lipids are phospholipid and unesterified cholesterol (UC), and the primary protein is APOA1 (1), encoded by the APOA1 gene in humans. Studies have established the association of low plasma HDL cholesterol concentration with an elevated risk of cardiovascular diseases (2), and more recently, the importance of HDL particle size in the plasma (3,4). The atheroprotective property of HDL is attributed to its role in a multistep process called reverse cholesterol transport (RCT) (5).…”

mentioning

confidence: 99%

Tertiary structure of apolipoprotein A-I in nascent high-density lipoproteins

Pourmousa

Song

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

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Understanding the function of high-density lipoprotein (HDL) requires detailed knowledge of the structure of its primary protein, apolipoprotein A-I (APOA1). However, APOA1 flexibility and HDL heterogeneity have confounded decades of efforts to determine high-resolution structures and consistent models. Here, molecular dynamics simulations totaling 30 μs on two nascent HDLs, each with 2 APOA1 and either 160 phospholipids and 24 cholesterols or 200 phospholipids and 20 cholesterols, show that residues 1-21 of the N-terminal domains of APOA1 interact via strong salt bridges. Residues 26-43 of one APOA1 in the smaller particle form a hinge on the disc edge, which displaces the C-terminal domain of the other APOA1 to the phospholipid surface. The proposed structures are supported by chemical cross-linking, Rosetta modeling of the N-terminal domain, and analysis of the lipid-free ∆185APOA1 crystal structure. These structures provide a framework for understanding HDL maturation and revise all previous models of nascent HDL.

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“…NMR, on the other hand, estimates b2.0 copies of apoA-I per particle, which is closer to the original IM method, but slightly lower than what the structural models suggest may be the true average. As the editorial that accompanied the journal article describing CIM illustrated, there are strengths and limitations to both NMR and CIM with respect to estimating circulating concentrations of HDL particles (Table 4) [37]. One of the limitations for either NMR or CIM measurement of HDL-P is that to date there has been no reference method with standard calibration of HDL particles in a complex solution such as serum to which an HDL-P assay could be compared for accuracy.…”

Section: Discussionmentioning

confidence: 99%

HDL particle number measured on the Vantera®, the first clinical NMR analyzer

Matyus¹,

Braun²,

Wolak-Dinsmore³

et al. 2015

Clinical Biochemistry

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HDL-C vs HDL-P: How Changing One Letter Could Make a Difference in Understanding the Role of High-Density Lipoprotein in Disease

Cited by 28 publications

References 14 publications

Serum Apolipoprotein A-I and Large High-Density Lipoprotein Particles Are Positively Correlated with FEV₁ in Atopic Asthma

Serum Apolipoprotein A-I and Large High-Density Lipoprotein Particles Are Positively Correlated with FEV₁ in Atopic Asthma

Tertiary structure of apolipoprotein A-I in nascent high-density lipoproteins

HDL particle number measured on the Vantera®, the first clinical NMR analyzer

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HDL-C vs HDL-P: How Changing One Letter Could Make a Difference in Understanding the Role of High-Density Lipoprotein in Disease

Cited by 28 publications

References 14 publications

Serum Apolipoprotein A-I and Large High-Density Lipoprotein Particles Are Positively Correlated with FEV1 in Atopic Asthma

Serum Apolipoprotein A-I and Large High-Density Lipoprotein Particles Are Positively Correlated with FEV1 in Atopic Asthma

Tertiary structure of apolipoprotein A-I in nascent high-density lipoproteins

HDL particle number measured on the Vantera®, the first clinical NMR analyzer

Contact Info

Product

Resources

About

Serum Apolipoprotein A-I and Large High-Density Lipoprotein Particles Are Positively Correlated with FEV₁ in Atopic Asthma

Serum Apolipoprotein A-I and Large High-Density Lipoprotein Particles Are Positively Correlated with FEV₁ in Atopic Asthma