HDL Composition Regulates Displacement of Cell Surface‐Bound Hepatic Lipase

Rouhani, Naghmeh; Young, Elizabeth; Chatterjee, Cynthia; Sparks, Daniel L.

doi:10.1007/s11745-008-3214-1

Cited by 19 publications

(31 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…36 Subjects with high postheparin HL activity would be expected to exhibit decreased vascular lipolysis and increased plasma TG levels. 2 Both the lipid and apoprotein components of HDL have been shown to affect its HL displacement ability 14 and HDL has been shown to displace HL similarly in both HepG2 and CHO-hHL cells. 13,14 To determine the effect of lipid enrichment on HL displacement, Rouhani et al enriched native and rHDL particles with various lipids and characterized HL displacement.…”

Section: Discussionmentioning

confidence: 99%

“…2 Both the lipid and apoprotein components of HDL have been shown to affect its HL displacement ability 14 and HDL has been shown to displace HL similarly in both HepG2 and CHO-hHL cells. 13,14 To determine the effect of lipid enrichment on HL displacement, Rouhani et al enriched native and rHDL particles with various lipids and characterized HL displacement. Enrichment with free fatty acids and cholesteryl esters had minimal effects on HL displacement; however, phospholipid and TG enrichment of reconstituted HDL blocked HL displacement.…”

Section: Discussionmentioning

confidence: 99%

“…This appears to agree with our previous reports, which have shown that HDL 2 is more effective at displacing HL from the surface of liver cells. 13,14 HL was shown to associate with HDL 2 after it is displaced from CHO-hHL cells by human HDL.…”

Section: Hypercholesterolemia Decreases Hl Displacement In Womenmentioning

confidence: 99%

“…12 A higher postheparin activity therefore appears to be a marker for a larger liver depot of inactive HSPG-bound HL. High-density lipoprotein (HDL) has been shown to displace HL from the cell surface much like heparin [12][13][14] ; however, the composition of the HDL can affect its ability to displace HL. While apolipoprotein A-I (apoA-I), apolipoprotein A-II (apoA-II) and apolipoprotein C-I (apoC-I) appear to stimulate HL displacement, other apoproteins had the opposite effect and blocked HDL from displacing cell surface HL.…”

mentioning

confidence: 99%

“…While apolipoprotein A-I (apoA-I), apolipoprotein A-II (apoA-II) and apolipoprotein C-I (apoC-I) appear to stimulate HL displacement, other apoproteins had the opposite effect and blocked HDL from displacing cell surface HL. [12][13][14] Apolipoprotein E (apoE) is an exchangeable apolipoprotein present on both HDL-and TG-rich lipoproteins. Plasma apoE levels appear to correlate to postheparin HL activity.…”

mentioning

confidence: 99%

See 4 more Smart Citations

HDL-ApoE Content Regulates the Displacement of Hepatic Lipase from Cell Surface Proteoglycans

Young

Chatterjee

Sparks

2009

The American Journal of Pathology

Self Cite

View full text Add to dashboard Cite

Human hepatic lipase (HL) is an interfacial enzyme that must be liberated from cell surface proteoglycans to hydrolyze lipoprotein triglyceride. Both high-density lipoprotein (HDL) and apolipoprotein (apo)A-I can displace HL from cell surface proteoglycans, much like heparin. HL displacement is inhibited by HDL-apoE content. Postprandial HDL is approximately twofold better at displacing HL than is fasting HDL, but only has approximately one-half the apoE content. Enriching native HDL with triglyceride decreases HDL-apoE content and increases HL displacement. Incubation of HDL with the anti-apoE antibody, 6C5, also increases HL displacement. In contrast, enrichment of synthetic HDL with apoE significantly inhibits HL displacement. HDL from fasted female normolipidemic subjects displaces HL approximately twofold better than HDL from male subjects. HDL from female subjects also has significantly less apoE than HDL from males. Normolipidemic females have increased circulating HDL-bound HL. Hyperlipidemia has little effect on the HL displacement ability of HDL from men, whereas HDL from hypercholesterolemic females exhibits impaired HL displacement. HL displacement from liver heparan sulfate proteoglycans therefore appears to be linked to interlipoprotein apoE exchange. Decreased HL displacement is associated with higher HDL-apoE levels and may therefore affect vascular triglyceride hydrolysis. (Am J Pathol

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Hypercholesterolemia Decreases Hl Displacement In Womenmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

HDL-ApoE Content Regulates the Displacement of Hepatic Lipase from Cell Surface Proteoglycans

Young

Chatterjee

Sparks

2009

The American Journal of Pathology

Self Cite

View full text Add to dashboard Cite

show abstract

Metabolism

2011

High‐Density Lipoproteins

View full text Add to dashboard Cite

Hepatic Lipase, High Density Lipoproteins, and Hypertriglyceridemia

Chatterjee

Sparks

2011

The American Journal of Pathology

Self Cite

111

View full text Add to dashboard Cite

Hepatic lipase (HL) is a lipolytic enzyme that contributes to the regulation of plasma triglyceride (TG) levels. Elevated TG levels may increase the risk of developing coronary heart disease, and studies suggest that mutations in the HL gene may be associated with elevated TG levels and increased risk of coronary heart disease. Hepatic lipase facilitates the clearance of TG from the very low density lipoprotein (VLDL) pool, and this function is governed by the composition and quality of high density lipoprotein (HDL) particles. In humans, HL is a liver resident enzyme regulated by factors that release it from the liver and activate it in the bloodstream. HDL regulates the release of HL from the liver and HDL structure controls HL transport and activation in the circulation. Alterations in HDL-apolipoprotein composition can perturb HL function by inhibiting the release and activation of the enzyme. HDL structure may therefore affect plasma TG levels and coronary heart disease risk. Triglycerides and Heart DiseaseElevated plasma triglyceride (TG) levels have been viewed as a risk factor for coronary heart disease (CHD) for more than a decade.1,2 Plasma TG levels are regulated by both synthesis and degradation of both very low density lipoprotein (VLDL) and chylomicron particles. The clearance of TG-rich lipoproteins from the circulation is controlled by the actions of lipoprotein lipase (LPL) and hepatic lipase (HL) and by the interlipoprotein exchange of TG by cholesteryl ester transfer protein. Lipoprotein lipase is the predominant TG lipase and is responsible for hydrolyzing TG in chylomicrons and VLDL, whereas HL is both a phospholipase and a TG lipase and plays an important role in HDL metabolism and in the conversion of VLDL to LDL.3 Single nucleotide polymorphisms (SNPs) in the HL gene (LIPC) have been shown to associate with plasma lipid concentrations and increased CHD risk. 4,5 Hepatic lipase deficiency is a result of relatively rare LIPC mutations that give rise to a loss in circulating HL activity (due to impaired secretion or inactive enzyme) and cause an increase in TG-rich HDL and VLDL remnants and increased CHD risk.

show abstract

HDL Composition Regulates Displacement of Cell Surface‐Bound Hepatic Lipase

Cited by 19 publications

References 50 publications

HDL-ApoE Content Regulates the Displacement of Hepatic Lipase from Cell Surface Proteoglycans

HDL-ApoE Content Regulates the Displacement of Hepatic Lipase from Cell Surface Proteoglycans

Metabolism

Hepatic Lipase, High Density Lipoproteins, and Hypertriglyceridemia

Contact Info

Product

Resources

About