HDL3, but not HDL2, stimulates plasminogen activator inhibitor-1 release from adipocytes: the role of sphingosine-1-phosphate

Lee, Mi-Hye; Hammad, Samar M.; Semler, Andrea J.; Luttrell, Louis M.; Lopes‐Virella, Maria F.; Klein, Richard L.

doi:10.1194/jlr.m003988

Cited by 48 publications

(38 citation statements)

References 53 publications

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“…The small, dense HDL particles, HDL3, carry 2- to 3-fold higher S1P levels compared with HDL2 on a molar basis (13)(14)(15)(16). It has been shown previously that several biological effects that are ascribed to HDL may be mediated by the S1P content of HDL through activation of S1PRs.…”

Section: Synthesis Of Recombinant Hdl and Recombinant Hdl Containing S1pmentioning

confidence: 99%

“…4). We have shown previously that HDL-mediated plasminogen activator inhibitor-1 secretion requires SR-BI and S1PR2 in 3T3L1 cells (16). It has been suggested that the binding of HDL to the SR-BI receptor could provide the necessary spatial proximity for HDL-bound S1P to effectively stimulate S1P receptors such that SR-BI is the docking receptor that captures HDL particles to facilitate the release of S1P to activate cellular S1P receptors (14,20).…”

Section: Interaction Between Sr-bi and S1pr1mentioning

confidence: 99%

“…The final concentrations in each well for each agonist are rHDL and rHDL + S1P (30 µg protein/ml), HDL2 and HDL3 (10, 50, and 200 µg/ml) in RVSM cells and (50,200, and 500 µg/ml) in HEK293, and S1P (0.1, 0.5, and 1 µM). The final concentration of S1P in the culture medium for cells incubated with rHDL + S1P was set at 120 µM as was used in our previous studies (16) to approximate S1P levels in HDL (15). For clarity, only data collected at even-numbered time points are plotted.…”

Section: Analysis Of Physical Interaction Between Hdl Receptor Sr-bi mentioning

confidence: 99%

“…We have previously shown that HDL mediates plasminogen activator inhibitor-1 secretion in 3T3L1 adipocytes through activation of S1PR2 (16) and determined that SR-BI is also required to affect this metabolism (unpublished observation). It has been hypothesized that the binding of HDL to the SR-B1 receptor may provide the necessary spatial geometry to bring HDL-bound S1P sufficiently close to its cognate receptors to initiate the various S1PR-mediated signaling cascades on the cell surface (20,22).…”

Section: Synthesis Of Recombinant Hdl and Recombinant Hdl Containing S1pmentioning

confidence: 99%

“…HDL2 and HDL3 were isolated from plasma as we described previously (16). Briefly, blood (200 ml) was collected in the presence of an anticoagulant/lipoprotein preservative cocktail [EDTA (0.1% w/v), chloramphenicol (20 µg/ml), gentamycin sulfate (50 µg/ml), epsilon--caproic acid (0.13% w/v), and dithiobisnitrobenzoic acid (0.04% w/v) to inhibit LCAT activity (final concentrations)] after an overnight fast from each of three or four donors who were free from clinically apparent disease.…”

Section: Isolation Of Hdl2 and Hdl3mentioning

confidence: 99%

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S1P in HDL promotes interaction between SR-BI and S1PR1 and activates S1PR1-mediated biological functions: calcium flux and S1PR1 internalization

Lee

Appleton

El‐Shewy

et al. 2017

Journal of Lipid Research

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Journal of Lipid Research Volume 58, 2017325 interaction between SR-BI and S1PR1 and activates S1PR1-mediated biological functions: calcium flux and S1PR1 internalization. J. Lipid Res. 2017. 58: 325-338.Supplementary key words high density lipoprotein • sphingosine 1-phosphate • protein-fragment complementation assay • scavenger receptor BI • S1P receptorsThe reverse transport of cholesterol from the periphery to the liver is often considered as the hallmark antiatherogenic function of HDL. SR-BI, the first molecularly characterized HDL receptor that plays a critical role in this metabolism, is abundantly expressed in several types of cells and tissue types, including the liver parenchyma, adrenal cortical cells, platelets, endothelial cells, smooth muscle cells, and macrophages. SR-BI facilitates the selective uptake of HDL cholesterol by cells, primarily in the form of cholesteryl esters. SR-BI also mediates the bidirectional flux of unesterified cholesterol and phospholipids between HDL and cells, which leads to an increase in cellular cholesterol mass and alters cholesterol distribution in plasma membrane domains (1-4).Numerous studies have detailed the various signaling pathways generated by the interaction of HDL with cell surface receptors that have been shown to induce myriad Abstract HDL normally transports about 50-70% of plasma sphingosine 1-phosphate (S1P), and the S1P in HDL reportedly mediates several HDL-associated biological effects and signaling pathways. The HDL receptor, SR-BI, as well as the cell surface receptors for S1P (S1PRs) may be involved partially and/or completely in these HDL-induced processes. Here we investigate the nature of the HDLstimulated interaction between the HDL receptor, SR-BI, and S1PR1 using a protein-fragment complementation assay and confocal microscopy. In both primary rat aortic vascular smooth muscle cells and HEK293 cells, the S1P content in HDL particles increased intracellular calcium concentration, which was mediated by S1PR1. Mechanistic studies performed in HEK293 cells showed that incubation of cells with HDL led to an increase in the physical interaction between the SR-BI and S1PR1 receptors that mainly occurred on the plasma membrane. Model recombinant HDL (rHDL) particles formed in vitro with S1P incorporated into the particle initiated the internalization of S1PR1, whereas rHDL without supplemented S1P did not, suggesting that S1P transported in HDL can selectively activate S1PR1. In conclusion, these data suggest that S1P in HDL stimulates the transient interaction between SR-BI and S1PRs that can activate S1PRs and induce an elevation in intracellular calcium concentration.-Lee,

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Section: Synthesis Of Recombinant Hdl and Recombinant Hdl Containing S1pmentioning

confidence: 99%

Section: Interaction Between Sr-bi and S1pr1mentioning

confidence: 99%

Section: Analysis Of Physical Interaction Between Hdl Receptor Sr-bi mentioning

confidence: 99%

Section: Synthesis Of Recombinant Hdl and Recombinant Hdl Containing S1pmentioning

confidence: 99%

Section: Isolation Of Hdl2 and Hdl3mentioning

confidence: 99%

See 3 more Smart Citations

S1P in HDL promotes interaction between SR-BI and S1PR1 and activates S1PR1-mediated biological functions: calcium flux and S1PR1 internalization

Lee

Appleton

El‐Shewy

et al. 2017

Journal of Lipid Research

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Biologic Activities

2011

High‐Density Lipoproteins

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Blood Sphingolipids in Homeostasis and Pathobiology

Hammad

2011

Advances in Experimental Medicine and Biology

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Sphingolipids have emerged as key signaling molecules involved in the regulation of a variety of cellular functions including cell growth and differentiation, proliferation and apoptotic cell death. Sphingolipids in blood constitute part of the circulating lipoprotein particles (HDL, LDL and VLDL), carried by serum albumin and also present in blood cells and platelets. Recent lipidomic and proteomic studies of plasma lipoproteins have provided intriguing data concerning the protein and lipid composition of lipoproteins in the context of disease. Sphingolipids have been implicated in several diseases such as cancer, obesity, atherosclerosis and sphingolipidoses; however, efforts addressing blood sphingolipidomics are still limited. The development of methods to determine levels of circulating bioactive sphingolipids in humans and validation of these methods to be a routine clinical laboratory test could be a pioneering approach to diagnose disease in the population. This approach would probably evolve to be analogous in implication to determining "good" and "bad" cholesterol and triglyceride levels in lipoprotein classes.

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HDL3, but not HDL2, stimulates plasminogen activator inhibitor-1 release from adipocytes: the role of sphingosine-1-phosphate

Cited by 48 publications

References 53 publications

S1P in HDL promotes interaction between SR-BI and S1PR1 and activates S1PR1-mediated biological functions: calcium flux and S1PR1 internalization

S1P in HDL promotes interaction between SR-BI and S1PR1 and activates S1PR1-mediated biological functions: calcium flux and S1PR1 internalization

Biologic Activities

Blood Sphingolipids in Homeostasis and Pathobiology

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