He–Ne laser (632.8 nm) pre‐irradiation gives protection against DNA damage induced by a near‐infrared trapping beam

Sahu, Khageswar; Mohanty, Samarendra; Gupta, Pradeep Kumar

doi:10.1002/jbio.200810041

Cited by 12 publications

(12 citation statements)

References 10 publications

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“…At least in one case pre-irradiation at 632.8 nm was employed to “prime” cells for later exposure to 1064 nm light. Cells exposed to 632.8 nm endured better the subsequent irradiation to 1064 nm [141]. Alternatively, optical tweezers working with actinic light can be put to use to assess non-adherent cell redox responses.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, Nd lasers, mainly Nd-YAG, are widely employed as sources for optical tweezers. However, several publications have reported chemical and biological damage at relatively low light intensities in optical traps at this wavelength [30], [31], [135], [136], [137], [138], [139], [140], [141], [142]. In many of these publications the source of damage has been ascribed to direct thermal action (due to water absorption), organic chromophore excitation in the limit of the absorption band ( e.g .…”

Section: Direct Optical Excitation Of 1o2mentioning

confidence: 99%

“…Several experimental aspects are detailed in each entry (see footnotes for more information). Each set of conditions has been obtained from a publication, with the particular reference provided in each case. Transition Biological model Light source Wavelength Power Spectral control 1 O 2 scavenging 1 O 2 enhancement Thermal control Reference 633 nm Human erythrocytesDye laser (pulsed)633 nm–YesNoNoNo[108]Human erythrocytesHe-Ne laser (cw)632.8 nm38–50 mWNopArpO 2 Yes[108]NC-37 lymphoblast lineHe-Ne laser (cw)633 nm1.2 mWNoNoNoNo[177]A2058 melanoma lineHe-Ne laser (cw)632.8 nm7 mWNoNoNoYes[178]Human skin fibroblastsHe-Ne laser (cw)632.8 nm18.8 mWYesNoNoYes[143]MCF-7 breast cancer lineHe-Ne laser (cw)632.8 nm14 mWNoNoNoNo[141]-A549Diode laser (cw)635 nm–No-SODNoNo[181]-EMT6-NAC-ASTC-a-1-EMT6 tumors in miceDiode laser (cw)635 nm–NoNoNoNo[181]-ASTC-a-1 tumors in mice-A549 adenocarcinomaLED (cw)637±17 nm–YesNoNoYes[146]-PtK2 renal line-U2OS osteosarcomaClinical trials in womenDiode laser (cw)…”

Section: Direct Optical Excitation Of 1o2mentioning

confidence: 99%

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Direct 1O2 optical excitation: A tool for redox biology

Blázquez‐Castro

2017

Redox Biology

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Molecular oxygen (O2) displays very interesting properties. Its first excited state, commonly known as singlet oxygen (1O2), is one of the so-called Reactive Oxygen Species (ROS). It has been implicated in many redox processes in biological systems. For many decades its role has been that of a deleterious chemical species, although very positive clinical applications in the Photodynamic Therapy of cancer (PDT) have been reported. More recently, many ROS, and also 1O2, are in the spotlight because of their role in physiological signaling, like cell proliferation or tissue regeneration. However, there are methodological shortcomings to properly assess the role of 1O2 in redox biology with classical generation procedures. In this review the direct optical excitation of O2 to produce 1O2 will be introduced, in order to present its main advantages and drawbacks for biological studies. This photonic approach can provide with many interesting possibilities to understand and put to use ROS in redox signaling and in the biomedical field.

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Section: Discussionmentioning

confidence: 99%

Section: Direct Optical Excitation Of 1o2mentioning

confidence: 99%

Section: Direct Optical Excitation Of 1o2mentioning

confidence: 99%

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Direct 1O2 optical excitation: A tool for redox biology

Blázquez‐Castro

2017

Redox Biology

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“…All these deleterious effects were significantly suppressed by the LLLI pretreatment. These results were obtained with different experimental conditions such as exposure time, incubation period between the LLL exposure, subsequent UV irradiation doses and wavelength and types of cells [27,28,30].…”

Section: Discussionmentioning

confidence: 99%

Low-level laser irradiation protects the chick embryo chorioallantoic membrane from UV cytotoxicity

Hammami¹,

Amri²,

Mokni³

2018

ARCH BIOL SCI BELGRA

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Low-level laser therapy or photobiomodulation is the medical use of a very low intensity light in the red to near infrared (wavelengths in the range of 630-940 nm). The present work was conducted to explore the effects of both UV and low-level laser irradiation (LLLI) on microcirculation using the in vivo model of the chick embryo chorioallantoic membrane (CAM). The effects were assessed by measuring lipid peroxidation and antioxidant enzyme activity. Cell cytotoxicity, survival and intracellular reactive oxygen species (ROS) of the CAM were also evaluated. We found that UV irradiation induced alterations of the vessels, leading to bleeding and extravasation. This effect was intensified after 60 min of exposure to UV irradiation, leading to marked edema. UVA irradiation increased cell cytotoxicity as assessed by lactate dehydrogenase (LDH) release (56.23% of control) and reduced cell viability as assessed by decreased fluorescein diacetate (FDA) fluorescence (56.23% of control). Pretreatment with LLLI prior to UV exposure protected the CAM tissue from UV-mediated cell death. This protective effect was supported by the observation of significantly inhibited lipid peroxidation (from 0.3±0.004 for UV, to 0.177±0.012 after LLLI pretreatment), ROS and O 2 production, as indicated by respective dihydrorhodamine (DHR) and dihydroethidium (DHE) intensities (from 132.78% of control for UVA, to 95.90% of control for L-UV (DHR), and from 127.34% of control for UVA, to 82.03% of control for L-UV (DHE)), and by preventing the increase in oxidative activities. LLLI efficiently protected CAM cells from UV-induced oxidative stress and appeared as a safe protective pretreatment against UV irradiation.

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“…When cells were preirradiated with a 632.8 nm source, a significantly greater number of cells survived subsequent optical tweezing at 1064 nm. 114 It has been proposed that the mechanism for preirradiation protection may involve activation of the cytochrome c. 115 Again in mammalian cells, light at optical tweezing wavelengths, has also been shown to induce an expression of heat shock proteins, 116 which are chaperone molecules that might aid the cell to repair light-denatured proteins.…”

Section: Viability Of Optically Manipulated Cellsmentioning

confidence: 99%

Light forces the pace: optical manipulation for biophotonics

2010

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Abstract. The biomedical sciences have benefited immensely from photonics technologies in the last 50 years. This includes the application of minute forces that enable the trapping and manipulation of cells and single molecules. In terms of the area of biophotonics, optical manipulation has made a seminal contribution to our understanding of the dynamics of single molecules and the microrheology of cells. Here we present a review of optical manipulation, emphasizing its impact on the areas of single-molecule studies and single-cell biology, and indicating some of the key experiments in the fields.

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He–Ne laser (632.8 nm) pre‐irradiation gives protection against DNA damage induced by a near‐infrared trapping beam

Cited by 12 publications

References 10 publications

Direct 1O2 optical excitation: A tool for redox biology

Direct 1O2 optical excitation: A tool for redox biology

Low-level laser irradiation protects the chick embryo chorioallantoic membrane from UV cytotoxicity

Light forces the pace: optical manipulation for biophotonics

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